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Molecular characterization of Cystoisospora belli and unizoite tissue cyst in patients with Acquired Immunodeficiency Syndrome

Published online by Cambridge University Press:  09 September 2010

JORGE NÉSTOR VELÁSQUEZ ,
GERMÁN ASTUDILLO OSVALDO ,
CECILIA DI RISIO ,
CRISTINA ETCHART ,
AGUSTÍN VÍCTOR CHERTCOFF ,
GLADYS ELISABET PERISSÉ  and
SILVANA CARNEVALE
JORGE NÉSTOR VELÁSQUEZ
Affiliation:
Hospital Municipal de Infecciosas ‘Dr. Francisco Javier Muñiz’, Uspallata 2272, (CP 1282) Ciudad de Buenos Aires, Argentina
GERMÁN ASTUDILLO OSVALDO
Affiliation:
Instituto Nacional de Enfermedades Infecciosas - ANLIS ‘Dr. Carlos G. Malbrán’, Avenida Vélez Sarsfield 563, (CP 1281) Ciudad de Buenos Aires, Argentina
CECILIA DI RISIO
Affiliation:
Hospital Municipal General de Agudos ‘Dr. José María Penna’, Pedro Chutro 3380, (CP 1437) Ciudad de Buenos Aires, Argentina
CRISTINA ETCHART
Affiliation:
Hospital Municipal General de Agudos ‘Dr. José María Penna’, Pedro Chutro 3380, (CP 1437) Ciudad de Buenos Aires, Argentina
AGUSTÍN VÍCTOR CHERTCOFF
Affiliation:
Instituto Nacional de Enfermedades Infecciosas - ANLIS ‘Dr. Carlos G. Malbrán’, Avenida Vélez Sarsfield 563, (CP 1281) Ciudad de Buenos Aires, Argentina
GLADYS ELISABET PERISSÉ
Affiliation:
Hospital Municipal General de Agudos ‘Dr. José María Penna’, Pedro Chutro 3380, (CP 1437) Ciudad de Buenos Aires, Argentina
SILVANA CARNEVALE*
Affiliation:
Instituto Nacional de Enfermedades Infecciosas - ANLIS ‘Dr. Carlos G. Malbrán’, Avenida Vélez Sarsfield 563, (CP 1281) Ciudad de Buenos Aires, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Avenida Rivadavia 1917, (CP 1033), Ciudad de Buenos Aires, Argentina
*
*Corresponding author: Av. Vélez Sarsfield 563, (CP 1281) Ciudad de Buenos Aires, Argentina. Tel: +54 11 4301 7437. E-mail: jorgeysilvana@speedy.com.ar
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Summary

Cystoisospora belli is a coccidian protozoan that can cause chronic diarrhoea, acalculous cholecystitis and cholangiopathy in AIDS patients. We applied molecular methods to identify Cystoisospora at species level in AIDS patients presenting with and without the presence of unizoites in lamina propria. Coprological and histological analyses were performed in stool and/or biopsy samples from 8 Cystoisospora-infected patients. DNA from the same samples was used to amplify 2 fragments of the SSU-rRNA gene and the ITS-1 region. Sequencing of the resulting amplicons identified C. belli infections in all cases, independent of the presence or absence of unizoite tissue cysts. Further work should be considered in order to find molecular targets related to strain variations in C. belli.

Keywords

Cystoisospora belliAIDSPCRunizoite tissue cyst
Type
Research Article
Information
Parasitology , Volume 138 , Issue 3 , March 2011 , pp. 279 - 286
Copyright
Copyright © Cambridge University Press 2010

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