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Molecular cloning and in vitro expression of C. elegans and parasitic nematode ionotropic receptors

Published online by Cambridge University Press:  06 April 2009

J. T. Fleming ,
H. A. Baylis ,
D. B. Sattelle  and
J. A. Lewis
J. T. Fleming
Affiliation:
Massachusetts General Hospital Cancer Center, Building 149, 13th Street, Charlestown, MA 02129, USA
H. A. Baylis
Affiliation:
The Babraham Institute Laboratory of Molecular Signalling, Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, UK
D. B. Sattelle*
Affiliation:
The Babraham Institute Laboratory of Molecular Signalling, Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, UK
J. A. Lewis
Affiliation:
Division of Life Sciences, University of Texas at San Antonio, San Antonio, Texas 78249-0662, USA
*
*Corresponding author.
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Summary

The free living nematode, C. elegans is understood at a level of detail equalled by few other organisms, and much of the cell biology and sequence information is proving of considerable utility in the study of parasitic nematodes. Already, C. elegans provides a convenient vehicle for investigating anthelmintic drug action and resistance mechanisms. Among the ionotropic receptors, with their important roles in the behaviour and development of the organism, are targets for anthelmintics. The subunits of nicotinic acetylcholine receptors of C. elegans form a large and diverse multigene family. Members of this family are among the 11 genes associated with resistance to the anthelmintic drug levamisole.

Keywords

Caenorhabditis elegansionotropic receptorsacetylcholine receptorsanthelmintic drug targetsresistance mechanisms
Type
Research Article
Information
Parasitology , Volume 113 , supplement S1: Molecular biochemistry and physiology of helminth neuromuscular systems , January 1996 , pp. S175 - S190
Copyright
Copyright © Cambridge University Press 1996

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