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Monoclonal antibodies to Leishmania tropica major: specificities and antigen location

Published online by Cambridge University Press:  06 April 2009

A. A. L. de Ibarra ,
J. G. Howard  and
D. Snary
A. A. L. de Ibarra
Affiliation:
Division of Experimental Biology, Wellcome Research Laboratories, Beckenham, Kent
J. G. Howard
Affiliation:
Division of Experimental Biology, Wellcome Research Laboratories, Beckenham, Kent
D. Snary
Affiliation:
Department of Immunochemistry, Wellcome Research Laboratories, Beckenham, Kent
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Extract

Six murine monoclonal antibodies to Leishmania tropica major have been prepared and the properties of these antibodies studied. Two (WIC 79.3 and 79.7) were L. tropica major species-specific and bound to promastigote cell surfaces, to parasitized macrophages, but not isolated amastigotes. No evidence was found for the production of antibody to the antigenic determinants recognized by WIC 79.3 or 79.7 during L. tropica major infections in mice and hamsters. One antibody (WIC 79.1) bound to sub-cellular organelles of Leishmania species but to a different sub-cellular organelle of Trypanosoma cruzi. Two others bound to the flagellum, one (WIC 79.2) to all Leishmania species, T. cruzi and Trypanosoma brucei, the other (WIC 79.4) only of L. tropica major and L. donovani species. One antibody (WIC 79.5) was directed against an unknown internal antigen found in all Leishmania species and T. cruzi

Type
Research Article
Information
Parasitology , Volume 85 , Issue 3 , December 1982 , pp. 523 - 531
Copyright
Copyright © Cambridge University Press 1982

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