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Morphological features of spermatozoa of Paragonimus ohirai (Trematoda: Platyhelminthes) examined by a silver nitrate staining technique

Published online by Cambridge University Press:  06 April 2009

H. Hirai  and
I. Tada
H. Hirai
Affiliation:
Department of Parasitic Diseases, Kumamoto University Medical School, 2-2-1 Honjo, Kumamoto, 860, Japan
I. Tada
Affiliation:
Department of Parasitic Diseases, Kumamoto University Medical School, 2-2-1 Honjo, Kumamoto, 860, Japan
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Extract

The mature thread-like spermatozoon of Paragonimus ohirai was shown to be composed of 7 distinct segments which have morphologically different characteristics, following staining with silver nitrate. The first segment (1S) is a needle-shaped structure with a perforatorium. The 2S is composed of two filaments loosely paired; 3S consists of a somewhat coarse thread (vestige of the cytoplasmic projection); 4S is composed of two flagella tightly paired; in 5S, two silver-positive filaments are independently seen as in 2S; 6S has a ‘spiral’ structure composed of two flagella; in 7S, two filaments are seen dissociated from each other. The sperm nucleus exists within the ‘spiral’ structure of 6S and this structure may serve to hold and/or protect the nucleus. The technique presented here is useful for gross morphological observations of the long sperm of flatworms.

Keywords

Paragonimus ohiraidigeneasilver nitrate stainingspermatozoonspermiogenesissperm nucleus
Type
Research Article
Information
Parasitology , Volume 103 , Issue 1 , August 1991 , pp. 103 - 110
Copyright
Copyright © Cambridge University Press 1991

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References

Burton, P. R. (1967). Fine structure of the reproductive system of a frog lung fluke. II. Penetration of the ovum by a spermatozoon. Journal of Parasitology 53, 994–9.Google Scholar
Burton, P. R. (1972). Fine structure of the reproductive system of a frog lung fluke. III. The spermatozoon and its differentiation. Journal of Parasitology 58, 68–83.Google Scholar
Burton, P. R. (1973). Some structural and cytochemical observations on the axial filament complex of lung fluke spermatozoa. Journal of Morphology 140, 185–96.CrossRefGoogle ScholarPubMed
Burton, P. R. & Silveira, M. (1972). Electron microscopic and optical diffraction studies of negatively stained axial units of certain platyhelminth sperm. Journal of Ultrastructure Research 36, 757–67.CrossRefGoogle Scholar
Elder, F. F. B. & Hsu, T. C. (1981). Silver-staining patterns of mammalian epididymal spermatozoa. Cytogenetics and Cell Genetics 30, 157–67.CrossRefGoogle ScholarPubMed
Fujino, T., Ishii, Y. & Mori, T. (1977). Ultrastructural studies on the spermatozoa and spermatogenesis in Paragonimus and Eurytrema (Trematoda: Digenea). Japanese Journal of Parasitology 26, 240–55. (In Japanese with English abstract.)Google Scholar
Goodpasture, C. & Bloom, S. E. (1975). Visualization of nucleolar organizer region in mammalian chromosomes using silver-staining. Chromosoma 53, 37–50.Google Scholar
Halton, D. W. & Hardcastle, A. (1976). Spermatogenesis in a monogenean, Diclidophora merlangi. International Journal for Parasitology 6, 43–53.CrossRefGoogle Scholar
Hirai, H. (1988). Paragonimus ohirai: identification of nucleolar organizer regions (NORs) and silver nitrate staining pattern in spermatogenesis. Experimental Parasitology 67, 281–6.Google Scholar
Hirai, H., Sakaguchi, Y., Habe, S. & Imai, H. T. (1985). C-banding analysis of six species of lung flukes, Paragonimus spp. (Trematoda: Platyhelminthes), from Japan and Korea. Zeitschrift für Parasitenkunde 71, 617–29.Google Scholar
Howell, W. M., Hsu, T. C. & Block, B. M. (1977). Visualization of centriole-bodies using silver-stain. Chromosoma 65, 9–20.CrossRefGoogle ScholarPubMed
Justine, J.-L. & Mattei, X. (1982 a). Etude ultrastructurale de la spermiogenèse et du spermatozoïde d'un Plathelminthe: Gonapodasmius (Trematoda: Didymozoidae). Journal of Ultrastructure Research 79, 350–65.CrossRefGoogle Scholar
Justine, J.-L. & Mattei, X. (1982 b). Réinvestigation de l'ultrastructure du spermatozoïde d'Haematoloechus (Trematoda: Haematoloechidae). Journal of Ultrastructure Research 81, 322–32.CrossRefGoogle ScholarPubMed
Justine, J.-L. & Mattei, X. (1983 a). Etude ultrastructurale comparée de la spermiogenèse des Monogènes. 1. Megalocotyle (Monopisthocotylea, Capsalidae). Journal of Ultrastructure Research 82, 296–308.Google Scholar
Justine, J.-L. & Mattei, X. (1993 b). Comparative ultrastructural study of spermiogenesis in monogeneans (flatworms). 2. Heterocotyle (Monopisthocotylea, Monocotylidae). Journal of Ultrastructure Research 84, 213–23.CrossRefGoogle Scholar
Justine, J.-L. & Mattei, X. (1983 c). Comparative ultrastructural study of spermiogenesis in monogeneans (flatworms). 3. Two species of Amphibdelloides (Monopisthocotylea, Amphibdellatidae). Journal of Ultrastructure Research 84, 224–37.CrossRefGoogle Scholar
Justine, J.-L. & Mattei, X. (1984 a). Comparative ultrastructural study of spermiogenesis in monogeneans (Flatworms). 4. Diplectanum (Monopisthocotylea, Diplectanidae). Journal of Ultrastructure Research 88, 77–91.Google Scholar
Justine, J.-L. & Mattei, X. (1984 b). Ultrastructural observations on the spermatozoon, oocyte and fertilization process in Gonapodasmius, a gonochoristic trematode (Trematoda: Digenea: Didymozoidae). Acta Zoologica 65, 171–7.Google Scholar
Orido, Y. (1988). Ultrastructure of spermatozoa of the lung fluke, Paragonimus ohirai (Trematoda: Troglotrematidae), in the seminal receptacle. Journal of Morphology 196, 333–43.Google Scholar
Sato, M., Oh, M. & Sakoda, K. (1967). Electron microscopic study of spermatogenesis in the lung fluke (Paragonimus miyazakii). Zeitschrift für Zellforschung 77, 232–43.Google Scholar
Shi, L., Hsu, T. C., Pathak, S. & Granovsky, T. A. (1982). Behavior of centrioles in spermatogenesis of the Madagascar hissing cockroach. Journal of Heredity 73, 408–12.Google Scholar
Shi, L., Pathak, S. & Hsu, T. C. (1982). Demonstration of kinetochores and centrioles in spermatocytes of two species of cockroaches by silver-staining. Chromosoma 85, 421–6.Google Scholar
Wolfe, A. D., Allison, R. G. & Hahn, F. E. (1972). Labilizing action of intercalating drugs and dyes on bacterial ribosomes. Biochemistry 9, 1569–72.CrossRefGoogle Scholar