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Natural IgM antibodies in sera from various animals but not the cat kill Toxoplasma gondii by activating the classical complement pathway

Published online by Cambridge University Press:  01 March 2004

Y. KANEKO ,
Y. TAKASHIMA ,
X. XUAUN ,
I. IGARASHI ,
H. NAGASAWA ,
T. MIKAMI  and
H. OTSUKA
Y. KANEKO
Affiliation:
Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
Y. TAKASHIMA
Affiliation:
Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
X. XUAUN
Affiliation:
National Research Center for Protozoan Disease, Obihiro University of Agricultural and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido 080-8555, Japan
I. IGARASHI
Affiliation:
National Research Center for Protozoan Disease, Obihiro University of Agricultural and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido 080-8555, Japan
H. NAGASAWA
Affiliation:
National Research Center for Protozoan Disease, Obihiro University of Agricultural and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido 080-8555, Japan
T. MIKAMI
Affiliation:
Nihon University College of Bioresource Sciences, 1866 Kameino, Fujisawa 252-8510, Japan
H. OTSUKA
Affiliation:
Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
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Abstract

Sera from swine, rabbit, and dog, that had never been exposed to Toxoplasma gondii, demonstrated significant killing of T. gondii tachyzoites in vitro, while cat serum did not. Swine and rabbit sera contained natural IgM antibody against the tachyzoites, and the classical complement pathway was activated by the binding of natural IgM antibody to the tachyzoites, leading to lysis. Anti-T. gondii antibodies, induced in swine or cat infected with T. gondii, had no killing effect by themselves but killed the tachyzoites in the presence of swine complement. However, the anti-T. gondii antibodies of swine or cat demonstrated a very low killing effect in the presence of cat complement. This suggests that T. gondii tachyzoites have an evasion mechanism to prevent lysis which is specific for cat complement.

Keywords

Toxoplasma gondiicomplementclassical pathwaynatural antibody
Type
Research Article
Information
Parasitology , Volume 128 , Issue 2 , February 2004 , pp. 123 - 129
Copyright
2004 Cambridge University Press

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References

REFERENCES

BOES, M. (2000). Role of natural and immune IgM antibodies in immune responses. Molecular Immunology 37, 1141–1149.CrossRefGoogle Scholar
FELDMAN, H. A. (1956). The relationship of Toxoplasma antibody activator to the serum-properdin system. Annals of the New York Academy of Sciences 66, 263–267.CrossRefGoogle Scholar
FUHRMAN, S. A. & JOINER, K. A. (1989). Toxoplasma gondii: mechanism of resistance to complement-mediated killing. Journal of Immunology 142, 940–947.Google Scholar
GERSTEN, D. M. & MARCHALONIS, J. J. (1978). A rapid, novel method for the solid-phase derivatization of IgG antibodies for immune-affinity chromatography. Journal of Immunological Methods 24, 305–309.CrossRefGoogle Scholar
GILLIN, F. D. & SHER, A. (1981). Activation of the alternative complement pathway by Trichomonas vaginalis. Infection and Immunity 34, 268–273.Google Scholar
HILL, R. D., BURGE, J. J. & PEARSON, R. D. (1984). Susceptibility of Giardia lamblia trophozoites to the lethal effect of human serum. Journal of Immunology 132, 2046–2052.Google Scholar
HOLBROOK, T. W., BOACKLE, R. J., PARKER, B. W. & VESELY, J. (1980). Activation of the alternative complement pathway by Naegleria fowleri. Infection and Immunity 30, 58–61.Google Scholar
HULDT, G., DAVIES, P., ALLISON, A. C. & SCHORLEMMER, H. U. (1979). Interactions between Entamoeba histolytica and complement. Nature, London 277, 214–216.CrossRefGoogle Scholar
INNES, A. E. (1997). Toxoplasmosis: comparative species susceptibility and host immune response. Comparative Immunology, Microbiology and Infectious Diseases 20, 131–138.CrossRefGoogle Scholar
KOMANIWA, H., FUKUSHO, A. & SHIMIZU, Y. (1987). Micro-method for neutralization test of transmissible gastroenteritis virus using kidney cell line CPK cells. Nippon Juigaku Zasshi 49, 141–144.CrossRefGoogle Scholar
KONISHI, E. (1991). Naturally occurring immunoglobulin M antibodies to Toxoplasma gondii in Japanese populations. Parasitology 102, 157–162.CrossRefGoogle Scholar
KONISHI, E. & NAKAO, M. (1992). Naturally occurring immunoglobulin M antibodies: enhancement of phagocytic and microbicidal activities of human neutrophils against Toxoplasma gondii. Parasitology 104, 427–432.CrossRefGoogle Scholar
MAEDA, K., HAYASHI, S., TANIOKA, Y., MATSUMOTO, Y. & OTSUKA, H. (2002). Pseudorabies virus is protected from complement attack by cellular factors and glycoprotein C. Virus Research 84, 79–87.CrossRefGoogle Scholar
ORTIZ-ORTIZ, L., CAPIN, R., CAPIN, N. R., SEPULVEDA, B. & ZAMACONA, G. (1978). Activation of the alternative pathway of complement by Entamoeba histolytica. Clinical and Experimental Immunology 34, 10–18.Google Scholar
PEARSON, R. D. & STEIGBIGEL, R. T. (1980). Mechanism of lethal effect of human serum upon Leishmania donovani. Journal of Immunology 125, 2195–2201.Google Scholar
ROOS, S. D., DONALD, R. G. K., MORRISSETTE, N. S. & MOULTON, A. L. C. (1994). Molecular tools for genetic dissection of the protozoan parasite Toxoplasma gondii. Methods in Cell Biology 45, 27–63.Google Scholar
SABIN, A. B. & FELDMAN, H. A. (1948). Dyes as microchemical indicators of a new immunity phenomenon affecting a protozoan parasite (Toxoplasma). Science 108, 660–663.CrossRefGoogle Scholar
SCHMUNIS, G. A. & HERMAN, R. (1970). Characteristics of so-called natural antibodies in various normal sera against culture forms of Leishmania. Journal of Parasitology 56, 889–896.CrossRefGoogle Scholar
SCHREIBER, R. D. & FELDMAN, H. A. (1980). Identification of the activator system for antibody to Toxoplasma as the classical complement pathway. Journal of Infectious Diseases 141, 366–369.CrossRefGoogle Scholar
SHAIO, M. & CHEN, J. (1989). Immunoglobulin M-dependent classical complement pathway activation in killing of Pentatrichomonas hominis. Infection and Immunity 57, 902–906.Google Scholar
STRANNEGARD, O. & LYCKE, E. (1966). Properdin and the antibody effect on Toxoplasma gondii. Acta Pathologica, Microbiologica et Immunologica Scandinavica 66, 227–238.CrossRefGoogle Scholar
SUZUKI, Y. & KOBAYASHI, A. (1985). Requirement for calcium ions in antibody-dependent complement mediated cytolysis of Toxoplasma gondii. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene 259, 426–431.CrossRefGoogle Scholar
TAKAHASHI, J. & KONISHI, E. (1986). Quantification of antibodies to Toxoplasma gondii in swine sera by enzyme-linked immunosorbent assay. Journal of Immunoassay 7, 257–272.CrossRefGoogle Scholar