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Naturally occurring immunoglobulin Mantibodies to Toxoplasma gondii in Japanese populations

Published online by Cambridge University Press:  06 April 2009

E. Konishi
E. Konishi
Affiliation:
Department of Medical Zoology, Kobe University School of Medicine, Kobe 650, Japan
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Summary

Varying degrees of naturally occurring immunoglobulin (Ig) M antibodies to Toxoplasma gondii were observed in almost all of adult Japanese populations negative for IgG antibodies as determined by an enzyme-linked immunosorbent assay (ELISA). The antibody activity was reduced by absorption of sera with Toxoplasma soluble antigen or by 2-mercapto-ethanol treatment of the IgM fraction after sucrose density gradient centrifugation. Umbilical cord sera had almost no detectable IgM antibodies, and the ELISA value in infants increased with age until 1 year. Most paired sera collected from adults at a 1-year interval showed constant IgM antibody levels, suggesting retention of natural IgM antibodies throughout their lives without changing ELISA values of ≥ 0·1/year. Polar staining was observed in immunofluorescent studies for 14 serum samples with natural IgM antibody levels of ≥ 0·5, while samples obtained from a pregnant woman with natural IgM antibody levels of ≥ 0·9 reacted to the whole organism as was observed in positive sera of acute patients. Western blotting analysis revealed that natural IgM antibodies were directed to multiple antigen molecules. The band pattern differed slightly from one serum to another, but almost consistent patterns were observed in sera sequentially obtained from an adult over 4 years.

Keywords

Toxoplasma gondiinatural antibodyimmunoglobulin Msero-epidemiology
Type
Research Article
Information
Parasitology , Volume 102 , Issue 2 , April 1991 , pp. 157 - 162
Copyright
Copyright © Cambridge University Press 1991

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