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Neutral glycolipids of Schistosoma mansoni as feasible antigens in the detection of schistosomiasis

Published online by Cambridge University Press:  06 April 2009

R. D. Dennis ,
S. Baumeister ,
G. Lauer ,
R. Richter  and
E. Geyer
R. D. Dennis*
Affiliation:
Biochemisches Institut am Klinikum der Universität Giessen, Friedrichstraβe 24, D-35385 Giessen, Germany
S. Baumeister
Affiliation:
Allgemeine und Medizinische Parasitologie, FB Biologie, Universität Marburg, D-35033 Marburg, Germany
G. Lauer
Affiliation:
Allgemeine und Medizinische Parasitologie, FB Biologie, Universität Marburg, D-35033 Marburg, Germany
R. Richter
Affiliation:
Allgemeine und Medizinische Parasitologie, FB Biologie, Universität Marburg, D-35033 Marburg, Germany
E. Geyer
Affiliation:
Allgemeine und Medizinische Parasitologie, FB Biologie, Universität Marburg, D-35033 Marburg, Germany
*
* Correspondence and reprint requests to Dr R. D. Dennis, Biochemisches Institut am Klinikum der Universität Giessen, Friedrichstraße 24, D-35385 Giessen, Germany.
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Summary

The neutral glycolipid fraction from mouse-propagated, Schistosoma mansoni adult worms has been investigated as to its chromatographic and antigenic properties, and whether it fulfills the serodiagnostic antigen requirements of sensitivity and specificity in the detection of schistosomiasis. Serological analyses were performed by thin-layer chromatography immunostaining and ELISA. In the acute-phase form of mouse schistosomiasis, the kinetics of development of neutral glycolipid-specific antibody levels was correlated with the intensity of the initial infection and the response was dominated by IgG, as represented by the subclass IgG1. With the experimental animal helminthiases screened, glycolipid antigenicity fulfilled the fundamental traits for a serodiagnostic reagent. In the chronic-phase form of human schistosomiasis mansoni, neutral glycolipid-specific antibody levels were not correlated with the intensity of infection, as estimated from the faecal content of parasite eggs, whilst the isotypic response was dominated by IgM and IgG, the latter represented primarily by IgG1 and secondarily by IgG3. With other human helminthiases, glycolipid antigenicity was incomplete, in that, the specificity was only partially fulfilled. The reason for this incomplete specificity has been clarified, in part, by the detection of cryptic schistosomiasis infections in the cohorts of African patient sera examined.

Keywords

trematodeSchistosoma mansoni adult glycolipidsHPTLC-immunostainingELISAdifferential sero-diagnosis.
Type
Research Article
Information
Parasitology , Volume 112 , Issue 3 , March 1996 , pp. 295 - 307
Copyright
Copyright © Cambridge University Press 1996

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