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New defined and semi-defined media for cultivation of the flagellate Trichomonas vaginalis

Published online by Cambridge University Press:  06 April 2009

David Linstead
David Linstead
Affiliation:
Department of Parasitology, Wellcome Research Laboratories, Langley Court, Beckenham, Kent BR3 3BS
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Summary

Studies on the nutrition and physiology of Trichomonas vaginalis have been hampered by the lack of a defined synthetic medium in which to grow the organism. New synthetic media free of ill-defined supplements and serum which support good growth of T. vaginalis have been developed in this study. The first successful approach to a defined medium resulted from the modification of the tissue-culture medium CMRL 1066. Essential modifications included (1) lowering the redox potential of the medium by adding high levels of ascorbate and (2) modifying the buffer system. Subsequently, CMRL 1066 was replaced by two new media DL7 and DL8 which have greater flexibility in their formulation and permit improved serum-free growth. Serum was replaced by bovine serum albumin and cholesterol, together with either a glyceryl fatty acid ester or a defined mixture of fatty acids. Preliminary nutritional studies using the new media have indicated a requirement for saturated and unsaturated fatty acids and a sterol.

Type
Research Article
Information
Parasitology , Volume 83 , Issue 1 , August 1981 , pp. 125 - 137
Copyright
Copyright © Cambridge University Press 1981

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