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Nitric oxide interaction with IL-10, MIP-1α, MCP-1 and RANTES over the in vitro granuloma formation against different Schistosoma mansoni antigenic preparations on human schistosomiasis

Published online by Cambridge University Press:  01 April 2000

D. M. OLIVEIRA ,
D. N. SILVA-TEIXEIRA ,
S. GUSTAVSON ,
S. M. P. OLIVEIRA  and
A. M. GOES
D. M. OLIVEIRA
Affiliation:
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, UFMG, Av. Antonio Carlos, 6627, Pampulha, C.P. 486, Belo Horizonte, Minas Gerais 30161–970 Brazil Departamento de Medicina Veterinária, Faculdade de Veterinária, Universidade Estadual do Ceará, UECE, Fortaleza, Ceará 60.740–000 Brazil
D. N. SILVA-TEIXEIRA
Affiliation:
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, UFMG, Av. Antonio Carlos, 6627, Pampulha, C.P. 486, Belo Horizonte, Minas Gerais 30161–970 Brazil
S. GUSTAVSON
Affiliation:
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, UFMG, Av. Antonio Carlos, 6627, Pampulha, C.P. 486, Belo Horizonte, Minas Gerais 30161–970 Brazil
S. M. P. OLIVEIRA
Affiliation:
Departamento de Zootecnia, Centro de Ciências Agrárias, Universidade Federal do Ceará, UFC, Fortaleza, Ceará 60.021–970 Brazil
A. M. GOES
Affiliation:
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, UFMG, Av. Antonio Carlos, 6627, Pampulha, C.P. 486, Belo Horizonte, Minas Gerais 30161–970 Brazil
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Abstract

Nitric oxide (NO) produced by cytokine-activated macrophages is reported to be cytotoxic against the helminth Schistosoma mansoni, although this is a controversial issue. Previous work in our laboratory identified a fraction of S. mansoni soluble adult worm antigenic preparation (SWAP), named PIII, able to elicit significant in vitro cell proliferation and at the same time lower in vitro and in vivo granuloma formation when compared either to soluble egg antigen (SEA) or to SWAP. Here we report that, in comparison to other S. mansoni antigenic preparations (SEA and SWAP), supernatants of PBMC cultivated with PIII possess higher concentrations of interleukin-10 (IL-10) and macrophage inflammatory protein (MIP-1α), concomitantly with lower concentrations of monocyte chemoattractant protein (MCP-1) and regulated on activation, normal T expressed and secreted (RANTES). In the particular case of NO inhibition, supernatants of PBMC cultivated with PIII present decreased IL-10 levels. Altogether, these results indicate that IL-10, MIP-1α, MCP-1 and RANTES are distinctively important elements in the PIII modulating role, while NO seems to be pivotal in the regulation of granulomatous responses.

Keywords

nitric oxideIL-10MIP-1αMCP-1RANTESin vitro granulomaschistosomiasis
Type
Research Article
Information
Parasitology , Volume 120 , Issue 4 , April 2000 , pp. 391 - 398
Copyright
2000 Cambridge University Press

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