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Novel insertions in the mitochondrial maxicircle of Trypanosoma musculi, a mouse trypanosome

Published online by Cambridge University Press:  04 August 2022

Ju-Feng Wang Open the ORCID record for Ju-Feng Wang [Opens in a new window] ,
Ruo-Hong Lin ,
Xuan Zhang ,
Geoff Hide Open the ORCID record for Geoff Hide [Opens in a new window] ,
Zhao-Rong Lun  and
De-Hua Lai Open the ORCID record for De-Hua Lai [Opens in a new window]
Ju-Feng Wang
Affiliation:
Guangdong Provincial Key Laboratory of Aquatic Economic Animals, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, The People's Republic of China
Ruo-Hong Lin
Affiliation:
Guangdong Provincial Key Laboratory of Aquatic Economic Animals, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, The People's Republic of China
Xuan Zhang
Affiliation:
Guangdong Provincial Key Laboratory of Aquatic Economic Animals, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, The People's Republic of China
Geoff Hide
Affiliation:
Ecosystems and Environment Research Centre and Biomedical Research Centre, School of Science, Engineering and Environment, University of Salford, Salford M5 4WT, UK
Zhao-Rong Lun*
Affiliation:
Guangdong Provincial Key Laboratory of Aquatic Economic Animals, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, The People's Republic of China Ecosystems and Environment Research Centre and Biomedical Research Centre, School of Science, Engineering and Environment, University of Salford, Salford M5 4WT, UK
De-Hua Lai*
Affiliation:
Guangdong Provincial Key Laboratory of Aquatic Economic Animals, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, The People's Republic of China
*
Author for correspondence: Zhao-Rong Lun, E-mail: lsslzr@mail.sysu.edu.cn; De-Hua Lai, E-mail: laidehua@mail.sysu.edu.cn
Author for correspondence: Zhao-Rong Lun, E-mail: lsslzr@mail.sysu.edu.cn; De-Hua Lai, E-mail: laidehua@mail.sysu.edu.cn
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Abstract

Trypanosoma musculi is a, globally distributed, mouse-specific haemoflagellate, of the family Trypanosomatidae, which shares similar characteristics in morphology with Trypanosoma lewisi. The kinetoplast (mitochondrial) DNA of Trypanosomatidae flagellates is comprised of catenated maxicircles and minicircles. However, genetic information on the T. musculi kinetoplast remains largely unknown. In this study, the T. musculi maxicircle genome was completely assembled, with PacBio and Illumina sequencing, and the size was confirmed at 34 606 bp. It consisted of 2 distinct parts: the coding region and the divergent regions (DRs, DRI and II). In comparison with other trypanosome maxicircles (Trypanosoma brucei, Trypanosoma cruzi and T. lewisi), the T. musculi maxicircle has a syntenic distribution of genes and shares 73.9, 78.0 and 92.7% sequence identity, respectively, over the whole coding region. Moreover, novel insertions in MURF2 (630 bp) and in ND5 (1278 bp) were found, respectively, which are homologous to minicircles. These findings support an evolutionary scenario similar to the one proposed for insertions in Trypanosoma cruzi, the pathogen of American trypanosomiasis. These novel insertions, together with a deletion (281 bp) in ND4, question the role of Complex I in T. musculi. A detailed analysis of DRII indicated that it contains numerous repeat motifs and palindromes, the latter of which are highly conservative and contain A5C elements. The comprehensively annotated kinetoplast maxicircle of T. musculi reveals a high degree of similarity between this parasite and the maxicircle of T. lewisi and suggests that the DRII could be a valuable marker for distinguishing these evolutionarily related species.

Keywords

Divergent regionsindelsmaxicircleminicirclepalindromeTrypanosoma lewisiTrypanosoma musculi
Type
Research Article
Information
Parasitology , Volume 149 , Issue 12 , October 2022 , pp. 1546 - 1555
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Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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Footnotes

*

Contributed equally.

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