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A novel member of the ligand-gated chloride channel gene family from Haemonchus contortus

Published online by Cambridge University Press:  20 December 2007

S. G. FORRESTER  and
S. Z. SIDDIQUI
S. G. FORRESTER*
Affiliation:
Faculty of Science, University of Ontario Institute of Technology, 2000 Simcoe Street North Oshawa, ON L1H 7K4, Canada
S. Z. SIDDIQUI
Affiliation:
Faculty of Science, University of Ontario Institute of Technology, 2000 Simcoe Street North Oshawa, ON L1H 7K4, Canada
*
*Corresponding author: Tel: +1 905 721 8668 ext 2937. Fax: +1 905 721 3304. E-mail: sean.forrester@uoit.ca
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Summary

Ligand-gated chloride channels (LGCCs) are key components of the nervous system of parasitic nematodes and important targets for anthelmintics. Here, we describe the isolation and characterization of a novel member of the LGCC gene family (HcLGCC1) from the parasitic nematode Haemonchus contortus. Sequence analysis revealed that the channel subunit encoded by HcLGCC1 is anion selective and a member of a group of channels characterized as having two Cys-loops in the N-terminal ligand-binding domain†. Although the overall function of HcLGCC1 is presently unknown, the gene may play a key role in the early developmental stages of the parasite. Further investigations into the function of LGCCs, such as HcLGCC1, in parasitic nematodes should have implications for the discovery of new anthelmintic targets.

Keywords

ligand-gated chloride channelneurotransmitter receptormolecular cloningHaemonchus contortus
Type
Original Articles
Information
Parasitology , Volume 135 , Issue 4 , April 2008 , pp. 539 - 545
Copyright
Copyright © Cambridge University Press 2007

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