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Occurrence of fibronectin antigenic determinants on Schistosoma mansoni lung schistosomula and adult worms

Published online by Cambridge University Press:  06 April 2009

M. A. Ouaissi ,
J. Cornette  and
A. Capron
M. A. Ouaissi
Affiliation:
Centre d'Immunologie et de Biologie Parasitaire, INSERM U 167, ERA CNRS 422, Institut Pasteur, 15 rue Camille Guérin, 59019 Lille Cédex, France
J. Cornette
Affiliation:
Centre d'Immunologie et de Biologie Parasitaire, INSERM U 167, ERA CNRS 422, Institut Pasteur, 15 rue Camille Guérin, 59019 Lille Cédex, France
A. Capron
Affiliation:
Centre d'Immunologie et de Biologie Parasitaire, INSERM U 167, ERA CNRS 422, Institut Pasteur, 15 rue Camille Guérin, 59019 Lille Cédex, France
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Summary

Fibronectin determinants were revealed in a soluble extract of Schistosoma mansoni adult worms by standard immunodiffusion and immunoelectrophoresis techniques. The same target molecules were demonstrated on the parasite's surface using a binding assay with [125I] anti-fibronectin. The use of fluorescein- and peroxidase-conjugated antibodies confirmed the above observations and provided a fairly precise means for locating the cross-reacting antigen on the worm surface. An in vitro cytotoxic assay using inactivated (56°C, 2 h) anti-fibronectin rabbit immune serum and guinea-pig serum as a source of complement was developed. In these conditions, anti-fibronectin exerted cytotoxic activity against lung schistosomula and adult worms but not skin schistosomula. The ultrastructural damage induced by anti-fibronectin in the presence of complement was studied using transmission electron microscopy. The results suggest a close association between fibronectin determinants and S. mansoni membrane.

Type
Research Article
Information
Parasitology , Volume 88 , Issue 1 , February 1984 , pp. 85 - 96
Copyright
Copyright © Cambridge University Press 1984

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