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Occurrence of Giardia and Cryptosporidium in wild birds in Galicia (Northwest Spain)

Published online by Cambridge University Press:  11 February 2015

AURORA REBOREDO-FERNÁNDEZ ,
ELVIRA ARES-MAZÁS ,
SIMONE M. CACCIÒ  and
HIPÓLITO GÓMEZ-COUSO
AURORA REBOREDO-FERNÁNDEZ
Affiliation:
Laboratory of Parasitology, Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Santiago de Compostela, Campus Vida, 15782 Santiago de Compostela, A Coruña, Spain
ELVIRA ARES-MAZÁS
Affiliation:
Laboratory of Parasitology, Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Santiago de Compostela, Campus Vida, 15782 Santiago de Compostela, A Coruña, Spain
SIMONE M. CACCIÒ
Affiliation:
Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy
HIPÓLITO GÓMEZ-COUSO*
Affiliation:
Laboratory of Parasitology, Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Santiago de Compostela, Campus Vida, 15782 Santiago de Compostela, A Coruña, Spain Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy
*
*Corresponding author. Laboratory of Parasitology, Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Santiago de Compostela, Campus Vida, 15782 Santiago de Compostela, A Coruña, Spain. E-mail: hipolito.gomez@usc.es
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Summary

Faecal samples were obtained from 433 wild birds being treated in wildlife recovery centres in Galicia (Northwest Spain), between February 2007 and September 2009. The birds belonged to 64 species representing 17 different orders. Giardia cysts and Cryptosporidium oocysts were detected by an immunofluorescence antibody test and identified at the molecular level by established PCR-sequencing methods. The overall prevalence of Giardia was 2·1% and that of Cryptosporidium, 8·3%. To our knowledge, this is the first description of Giardia sp. in Tyto alba and Caprimulgus europaeus; and of Cryptosporidium sp. in Apus apus, Athene noctua, C. europaeus, Falco tinnunculus, Morus bassanus, Parabuteo unicinctus and Strix aluco. Furthermore, the first PCR-sequence confirmed detection of Giardia duodenalis assemblage B in, Buteo buteo, Coturnix coturnix and Pica pica; G. duodenalis assemblage D in Garrulus glandarius; and G. duodenalis assemblage F in Anas platyrhynchos; Cryptosporidium parvum in Accipiter nisus, B. buteo, Milvus migrans, Pernis apivorus and P. pica; and Cryptosporidium meleagridis in Streptopelia turtur. The study findings demonstrate the wide spread of Giardia and Cryptosporidium between wild birds.

Keywords

wild birdsGiardiaCryptosporidiumimmunofluorescence microscopymolecular characterizationGalicia (Northwest Spain)
Type
Research Article
Information
Parasitology , Volume 142 , Issue 7 , June 2015 , pp. 917 - 925
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Copyright
Copyright © Cambridge University Press 2015 

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