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On the permeability of cestodes to colloids: an evaluation of the transmembranosis hypothesis*

Published online by Cambridge University Press:  06 April 2009

Richard D. Lumsden ,
Lawrence T. Threadgold ,
John A. Oaks  and
C. Arme
Richard D. Lumsden
Affiliation:
Laboratory of Parasitology, Department of Biology, Tulane University, New Orleans, Louisiana, U.S.A.
Lawrence T. Threadgold
Affiliation:
the Department of Biology, Rice University, Houston, Texas, U.S.A.
C. Arme
Affiliation:
the Department of Biology, Rice University, Houston, Texas, U.S.A.
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Summary

Adult Hymenolepis diminuta were tested for their ability to transport colloids under in vitro and in vivo conditions. The tracers employed included colloidal thorium dioxide (Thorotrast), carbon (Pelikan Ink), ferritin and the 14C-labelled protein fraction from Chlorella vulgaris. Though certain of these tracers were adsorbed to the external surface of the tegument plasmalemma, no evidence for the assimilation of any of these colloids was obtained in the present study. Further, significant differences in size and structure between the granular inclusions reported earlier by Rothman (1967) as assimilated colloids and the particles present in the respective colloidal suspensions were noted. Results of this investigation are interpreted as rendering untenable previous conclusions concerning transmembranosis of colloids by tapeworms.

Type
Research Article
Information
Parasitology , Volume 60 , Issue 2 , April 1970 , pp. 185 - 193
Copyright
Copyright © Cambridge University Press 1970

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References

Arme, C. & Read, C. P. (1968). Studies on membrane transport: II. The absorption of acetate and butyrate by Hymenolepis diminuta (Cestoda). Biological Bulletin, Marine Biological Laboratory, Woods Hole, Mass. 135, 80–91.CrossRefGoogle ScholarPubMed
Björkman, N. & Thorsell, W. (1964). On the fine structure and resorptive function of the cuticle of the liver fluke, Fasciola hepatica L. Experimental Cell Research 33, 319–29.CrossRefGoogle Scholar
Bogitsh, B. (1968). Cytochemical and ultrastructural observations on the tegument of the trematode Megalodiscus temperatus. Transactions of the American Microscopical Society 87, 477–86.CrossRefGoogle ScholarPubMed
Dike, S. C. (1969). Acid phosphatase activity and ferritin incorporation in the ceca of digenetic trematodes. Journal of Parasitology 55, 111–23.CrossRefGoogle Scholar
Graham, R. & Karnovsky, M. J. (1966). The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. Journal of Histochemistry & Cytochemistry 14, 291–302.CrossRefGoogle ScholarPubMed
Kilejian, A. (1966). Permeation of L-proline in the cestode, Hymenolepis diminuta. Journal of Parasitology 52, 1108–15.CrossRefGoogle ScholarPubMed
Kubish, W. (1967). Phagocytosis of colloidal thorium dioxide and cobaltic oxide by the reticuloendothelial system of the rat. Annals of the New York Academy of Sciences 145, 585–94.CrossRefGoogle Scholar
Luft, J. (1961). Improvement of epoxy resin embedding methods. Journal of Biophysical and Biochemical Cytology 4, 409–14.CrossRefGoogle Scholar
Lumsden, R. D. (1966 a). Cytological studies on the absorptive surfaces of cestodes. I. Fine structure of the strobilar integument. Zeitschrift für Parasitenkunde 27, 355–82.CrossRefGoogle ScholarPubMed
Lumsden, R. D. (1966 b). Cytological studies on the absorptive surfaces of cestodes. II. The synthesis and intracellular transport of protein in the strobilar integument of Hymenolepis diminuta. Zeitschrift für Parasitenkunde 28, 1–13.Google ScholarPubMed
Lumsden, R. D. (1969). Preparatory technique for electron microscopy. In Experiments and Techniques in Parasitology. Ed. Maclnnis, A. and Voge, M.. San Francisco: Freeman Press.Google Scholar
MacInnis, A., Fisher, F. M. Jr & Read, C. P. (1965). Membrane transport of purine and pyrimidines in a cestode. Journal of Parasitology 51, 260–7.CrossRefGoogle Scholar
Phifer, K. O. (1960 a). Permeation and membrane transport in animal parasites: the absorption of glucose by Hymenolepis diminuta. Journal of Parasitology 46, 51–62.CrossRefGoogle ScholarPubMed
Phifer, K. O. (1960 b). Permeation and membrane transport in animal parasites: further observations on the uptake of glucose by Hymenolepis diminuta. Journal of Parasitology 46, 137–44.CrossRefGoogle ScholarPubMed
Read, C. P. (1961). Competitions between sugars in their absorption by tapeworms. Journal of Parasitology 47, 1015–16.CrossRefGoogle ScholarPubMed
Read, C. P., Rothman, A. & Simmons, J. (1963). Studies on membrane transport with special reference to parasite-host integration. Annals of the New York Academy of Sciences 113, 154–205.CrossRefGoogle ScholarPubMed
Read, C. P., Simmons, J. & Rothman, A. (1960). Permeation and membrane transport in animal parasites: amino acid permeation into tapeworms from elasmobranchs. Journal of Parasitology 46, 33–41.CrossRefGoogle ScholarPubMed
Reynolds, E. (1963). The use of lead citrate at high pH as an electron opaque stain in electron microscopy. Journal of Cell Biology 17, 208–12.CrossRefGoogle ScholarPubMed
Rothman, A. H. (1963). Electron microscopic studies of tapeworms: the surface structure of Hymenolepis diminuta (Rudolphi, 1819) Blanchard, 1891. Transactions of the American Microscopical Society 82, 22–30.CrossRefGoogle Scholar
Rothman, A. H. (1967). Colloid transport in the cestode Hymenolepis diminuta. Experimental Parasitology 21, 133–6.CrossRefGoogle ScholarPubMed
Rothman, A. H. (1968). Enzyme localization and colloid transport in Haematoloechus medio-plexus. Journal of Parasitology 54, 286–94.CrossRefGoogle Scholar
Ryser, H. (1967). Studies on protein uptake by isolated tumor cells. III. Apparent stimulation due to pH, hypertonicity, polycations, or dehydration and their relation to the enhanced penetration of infectious nucleic acids. Journal of Cell Biology 32, 737–50.CrossRefGoogle Scholar
Schiller, E. (1965) A simplified method for the in vitro cultivation of the rat tapeworm, Hymenolepis diminuta. Journal of Parasitology 51, 516–18.CrossRefGoogle ScholarPubMed
Smith, J., Reynolds, E. & von Lichtenberg, F. (1969). The integument of Schistosoma mansoni. American Journal of Tropical Medicine and Hygiene 18, 28–49.CrossRefGoogle ScholarPubMed
Tanaka, H. (1962). Electron microscopic studies on vital stain as compared with phagocytosis. A concept of segresomes. Fifth International Congress of Electron Microscopy. LL-13.CrossRefGoogle Scholar
Tormey, J. (1965). Artifactual localization of ferritin in the ciliary epithelium in vitro. Journal of Cell Biology 25, 1–7.CrossRefGoogle ScholarPubMed
Watson, M. (1958). Staining of tissue sections for electron microscopy with heavy metals. Journal of Biophysical and Biochemical Cytology 4, 475–8.CrossRefGoogle ScholarPubMed