Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-15T03:39:51.521Z Has data issue: false hasContentIssue false

The organization of the ookinete and observations on nuclear division in oocysts of Plasmodium berghei

Published online by Cambridge University Press:  06 April 2009

Elizabeth U. Canning
Affiliation:
Department of Zoology and Applied Entomology, Imperial College, London
R. E. Sinden
Affiliation:
Department of Zoology and Applied Entomology, Imperial College, London

Extract

Ultramicroscopic features of the ookinete/oocyst transformation in Plasmodium berghei are described. The apical complex of organelles and some pellicular components, believed to be responsible respectively for cell penetration and body form were resorbed into the cytoplasm shortly after the ookinete came to rest under the basal lamina of the midgut wall. Within a single digitate nucleus, spindles at different phases of division were observed with kinetochores in early and late anaphase position. From the number of kinetochores the chromosome complement was estimated at 5–10.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1973

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aikawa, M. (1971). Plasmodium: The fine structure of malaria parasites. Experimental Parasitology 30, 284320.CrossRefGoogle Scholar
Aikawa, M. & Beaudoin, R. L. (1968). Studies on nuclear division of a malaria parasite under pyrimethamine treatment. Journal of Cell Biology 39, 749–54.CrossRefGoogle Scholar
Bano, L. (1959). A cytological study of the early oocysts of seven species of Plasmodium and the occurrence of post-zygotic meiosis. Parasitology 49, 559–85.CrossRefGoogle ScholarPubMed
Canning, E. U. & Anwar, M. (1968). Studies on meiotic division in coccidial and malarial parasites. Journal of Protozoology 15, 290–8.CrossRefGoogle ScholarPubMed
Canning, E. U. & Sinden, R. E. (1971). Nuclear division during sporogony in Plasmodium berghei. Comptes-Rendus des Multicolloque européen de Parasitologie 240–1.Google Scholar
Garnham, P. C. C. (1965). The structure of the early sporogonic stages of Plasmodium berghei. Annales de la Société belge de médecine tropicale 45, 259–66.Google ScholarPubMed
Garnham, P. C. C, Bird, R. G., Baker, J. R., Desser, S. S. & El-Nahal, H. M. S. (1969). Electron microscopic studies on motile stages of malaria parasites. VI. The ookinete of Plasmodium berghei yoelii and its transformation into the early oocyst. Transactions of the Royal Society of Tropical Medicine and Hygiene 63, 187–94.CrossRefGoogle ScholarPubMed
Howells, R. E. & Davies, E. E. (1971). Nuclear division in the oocyst of Plasmodium berghei. Annals of Tropical Medicine and Parasitology 65, 451–9.CrossRefGoogle ScholarPubMed
Inoué, S. (1953). Polarization optical studies of the mitotic spindle. I. The demonstration of spindle fibers in living cells. Chromosoma 5, 487500.CrossRefGoogle ScholarPubMed
Jokelainen, P. T. (1967). The ultrastructure and spatial organisation of metaphase kinetochores in mitotic rat cells. Journal of Ultrastructure Research 19, 1944.CrossRefGoogle ScholarPubMed
Ladda, R. L. (1969). New insights into the fine structure of rodent malaria parasites. Military Medicine 134, 825–64.CrossRefGoogle Scholar
Luykx, P. (1970). Cellular Mechanisms of Chromosome Distribution. International Review of Cytology, Supplement 2yes. Academic Press.Google ScholarPubMed
Reynolds, E. S. (1963). The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Journal of Cell Biology 17, 208–12.CrossRefGoogle ScholarPubMed
Satir, P. (1968). Studies on Cilia III. Further studies on the cilium tip and a ‘sliding filament’ model of ciliary motility. Journal of Cell Biology 39, 7794.CrossRefGoogle Scholar
Scalzi, H. A. & Bahr, G. F. (1968). An electron microscopic examination of erythrocytic stages of two rodent malaria parasites, Plasmodium chaubaudi and Plasmodium vinkei. Journal of Ultrastructure Research 24, 116–33.CrossRefGoogle Scholar
Schrader, P. (1944). Mitosis. The movements of chromosomes in cell division. 2nd Edition. Columbia University Press.Google Scholar
Terzakis, J. A. (1971). Transformation of the Plasmodium cynomolgi oocyst. Journal of Protozoology 18, 6273.CrossRefGoogle ScholarPubMed
Vanderberg, J. & Rhodin, J. (1967). Differentiation of nuclear and cytoplasmic fine structure during sporogonic development of Plasmodium berghei. Journal of Cell Biology 32, C710.CrossRefGoogle ScholarPubMed
Vanderberg, J., Rhodin, J. & Yoeli, M. (1967). Electron microscopic and histochemical studies of sporozoite formation in Plasmodium berghei. Journal of Protozoology 14, 82103.CrossRefGoogle Scholar
Walliker, D., Carter, R. & Morgan, S. (1971). Genetic recombination in malaria parasites. Nature, London 232, 561–2.CrossRefGoogle ScholarPubMed
Watson, M. L. (1958). Staining of tissue sections for electron microscopy with heavy metals. Journal of Biophysical and Biochemical Cytology 4, 475–8.CrossRefGoogle ScholarPubMed
Wolcott, G. B. (1954). Nuclear fine structure and division in the malaria parasite Plasmodium vivax. Journal of Morphology 94, 353–65.CrossRefGoogle Scholar
Wolcott, G. B. (1957). Chromosome studies in the genus Plasmodium. Journal of Protozoology 4, 4851.CrossRefGoogle Scholar
Yoeli, M. & Most, H. (1960). The biology of a newly isolated strain of Plasmodium berghei in a rodent host and experimental mosquito vectors. Transactions of the Royal Society of Tropical Medicine and Hygiene 54, 549–55.CrossRefGoogle Scholar