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Patterns of Onchocerca volvulus recombinant antigen recognition in a bovine model of onchocerciasis

Published online by Cambridge University Press:  16 October 2009

S. P. GRAHAM ,
Y. WU ,
K. HENKLE-DUEHRSEN ,
S. LUSTIGMAN ,
T. R. UNNASCH ,
G. BRAUN ,
S. A. WILLIAMS ,
J. McCARTHY ,
A. J. TREES  and
A. E. BIANCO
S. P. GRAHAM
Affiliation:
Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK
Y. WU
Affiliation:
Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK
K. HENKLE-DUEHRSEN
Affiliation:
Genetic Parasitology, Institute for Genetics, Heinrich-Heine-University, Universitätsstrasse 1, D-40225, Dusseldorf, Germany
S. LUSTIGMAN
Affiliation:
Lindsley F. Kimball Research Institute, New York Blood Centre, New York, NY, USA
T. R. UNNASCH
Affiliation:
Division of Geographic Medicine, BBRB 206, University of Alabama at Birmingham, Birmingham, AL 35294, USA
G. BRAUN
Affiliation:
Department of Pathology, University of Cambridge, Cambridge C2B 1QP, UK
S. A. WILLIAMS
Affiliation:
Department of Biological Sciences, Smith College, Northampton, MA 01063, USA
J. McCARTHY
Affiliation:
University Department of Medicine, Fremantle Hospital, P.O. Box 480, Fremantle WA 6959, Australia
A. J. TREES
Affiliation:
Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK
A. E. BIANCO
Affiliation:
Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK
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Abstract

The antibody responses of 8 cattle experimentally infected with Onchocerca ochengi to 18 recombinant O. volvulus antigens were measured by ELISA. In addition to establishing antigenic cross-reactivity between the species, the dynamics of antigen-specific responses were examined to assess how the recognition of the antigens compared to the known stage-specificity of expression. Six cattle responded to all of the antigens and 2 animals responded to all but 1. The dynamics of the recognition of 4 antigens (B20, MOv-2, MOv-14 and OvNHR2 02E1) were characterized by rapid seroconversion following infection. Antibody levels to 2 antigens (Ov7 and OvALT-1) increased gradually over the course of infection. Antibody levels to 4 antigens (OvTPX-2, OvL3Chitinase, Ov103 and Ov9m) reached maximum levels coincident with the onset of patency. The levels to 3 antigens (OvProalf C50, OvAldolase, Ov39) varied little over the course of infection. Responses to antigens with functional similarities (OvSOD1, OvSOD2 and OvSOD3 or OvGST1 and OvGST2) showed comparable temporal profiles. This study demonstrates the high degree of immunological cross-reactivity between the antigens of O. volvulus and O. ochengi. The immunogenicity of antigens varied over the course of infection in an antigen-specific manner, which not always reflected developmentally regulated expression of the corresponding gene, possibly owing to cross-reactive epitopes on distinct parasite products.

Keywords

Onchocerca ochengiOnchocerca volvuluscattle modelrecombinant antigensserological responses
Type
Research Article
Information
Parasitology , Volume 119 , Issue 6 , December 1999 , pp. 603 - 612
Copyright
© 1999 Cambridge University Press

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