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Periodate-sensitive immunological cross-reactivity between keyhole limpet haemocyanin (KLH) and serodiagnostic Schistosoma mansoni egg antigens

Published online by Cambridge University Press:  01 January 1999

J. V. HAMILTON ,
P. L. CHIODINI ,
P. G. FALLON  and
M. J. DOENHOFF
J. V. HAMILTON
Affiliation:
School of Biological Sciences, University of Wales, Bangor, Gwynedd LL57 2UW, UK
P. L. CHIODINI
Affiliation:
Department of Clinical Parasitology, Hospital for Tropical Diseases, 4 St Pancras Way, London NW1 0PE, UK
P. G. FALLON
Affiliation:
School of Biological Sciences, University of Wales, Bangor, Gwynedd LL57 2UW, UK Present address: Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK.
M. J. DOENHOFF
Affiliation:
School of Biological Sciences, University of Wales, Bangor, Gwynedd LL57 2UW, UK
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Abstract

Both CEF6, a cation-exchange fraction of soluble Schistosoma mansoni egg antigens (SEA), composed of the 2 antigens, alpha-1 and omega-1, and haemocyanin from the keyhole limpet, Megathura crenulata, have shown potential for immunodiagnosis of human schistosomiasis. Possible cross-reactivity between antigens in SEA and keyhole limpet haemocyanin (KLH) was explored by Western immunoblotting and enzyme-linked immunosorbent assay (ELISA) using sera from rabbits immunized with KLH, SEA, CEF6, alpha-1, omega-1, or egg antigen k5. Both immunoassays revealed a high degree of serological cross-reactivity between the schistosome egg antigens and KLH, much of it due to sodium periodate- sensitive epitopes. Cross-reactivity with schistosome antigens with proven diagnostic efficacy may thus, in part, explain the usefulness of KLH for the diagnosis of human schistosomiasis mansoni.

Keywords

serodiagnosiskeyhole limpet haemocyanin (KLH)CEF6cross-reactivity
Type
Research Article
Information
Parasitology , Volume 118 , Issue 1 , January 1999 , pp. 83 - 89
Copyright
1999 Cambridge University Press

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