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Phagocytosis and killing of Trypanosoma dionisii by human neutrophils, eosinophils and monocytes

Published online by Cambridge University Press:  06 April 2009

Kareen J. I. Thorne ,
Audrey M. Glauert ,
Rosamund J. Svvennsen  and
David Franks
Kareen J. I. Thorne
Affiliation:
Strangeways Research Laboratory, Worts' Causeway, Cambridge CB1 4RN
Audrey M. Glauert
Affiliation:
Strangeways Research Laboratory, Worts' Causeway, Cambridge CB1 4RN
Rosamund J. Svvennsen
Affiliation:
Department of Pathology, University of Cambridge, Cambridge, CB2 1PQ
David Franks
Affiliation:
Department of Pathology, University of Cambridge, Cambridge, CB2 1PQ
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Summary

The cell-mediated resistance of human leucocytes to Trypanosoma dionisii, a bat parasite related to T. cruzi, was investigated. Human peripheral blood neutrophils and monocytes were cytotoxic to T. dionisii as assessed by electron microscopy and by induction of 99mTc release from trypanosomes pre-labelled with [99mTc]pertechnetate. The enhancement of cytotoxicity by specific antiserum varied considerably from one individual to another. Neither blood lymphocytes nor blood eosinophils induced 99mTc release from T. dionisii. The trypanosomes were readily phagocytosed by neutrophils and monocytes even in the absence of added antiserum but the rate was enhanced when antiserum was present. Eosinophils also phagocytosed T. dionisii but only in the presence of antiserum. Investigation by electron microscopy revealed that T. dionisii is rapidly destroyed in the phagocytic vacuole of neutrophils and monocytes and by eosinophils. Phagocytosis, ultrastructural damage and induction of 99mTc release occurred more rapidly in neutrophils than in monocytes.

Type
Research Article
Information
Parasitology , Volume 79 , Issue 3 , December 1979 , pp. 367 - 379
Copyright
Copyright © Cambridge University Press 1979

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