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Phylogeny and morphology of Ovipleistophora diplostomuri n. sp. (Microsporidia) with a unique dual-host tropism for bluegill sunfish and the digenean parasite Posthodiplostomum minimum (Strigeatida)

Published online by Cambridge University Press:  12 July 2017

J. LOVY  and
S. E. FRIEND
J. LOVY*
Affiliation:
Office of Fish and Wildlife Health and Forensics, N.J. Division of Fish and Wildlife, 605 Pequest Road, Oxford, NJ 07863, USA
S. E. FRIEND
Affiliation:
Office of Fish and Wildlife Health and Forensics, N.J. Division of Fish and Wildlife, 605 Pequest Road, Oxford, NJ 07863, USA
*
*Corresponding author: Office of Fish and Wildlife Health and Forensics, New Jersey Division of Fish and Wildlife, 605 Pequest Road, Oxford, NJ 07863, USA. E-mail: Jan.Lovy@dep.nj.gov
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Summary

Microsporidia are diverse opportunistic parasites abundant in aquatic organisms with some species hyperparasitic in digenean parasites. In the current study, we describe a unique microsporidian parasite, Ovipleistophora diplostomuri n. sp. that has a tropism for both the bluegill sunfish Lepomis macrochirus, and its digenean parasite Posthodiplostomum minimum. Though the microsporidium first infects a fish, the subsequent infection causes hypertrophy of the metacercarial wall and degeneration of the P. minimum metacercariae within the fish tissue. Genetic analysis placed this species within Ovipleistophora and ultrastructural characteristics were consistent with the genus, including the presence of dimorphic spores within sporophorous vesicles. Meronts did not have a surface coat of dense material, which has been previously reported for the genus. This is the first Ovipleistophora species described that does not have a tropism for ovary. Genetics demonstrated that O. diplostomuri n. sp. groups closely within fish microsporidia and not other species known to be hyperparasitic in digeneans, suggesting that it evolved from fish-infecting microsporidians and developed a secondary tropism for a common and widespread digenean parasite. The high genetic identity to Ovipleistophora species demonstrates the close relationship of this unique microsporidian with other microsporidia that infect ovary.

Keywords

MicrosporidiaOvipleistophorabluegill sunfish Lepomis macrochirusdigeneanPosthodiplostomum minimumultrastructurephylogenetic analysis
Type
Research Article
Information
Parasitology , Volume 144 , Issue 14 , December 2017 , pp. 1898 - 1911
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Copyright
Copyright © Cambridge University Press 2017 

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