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Processing of the precursor to the major merozoite surface antigens of Plasmodium falciparum

Published online by Cambridge University Press:  06 April 2009

A. A. Holder ,
J. S. Sandhu ,
Yvonne Hillman ,
Lynne S. Davey ,
S. C. Nicholls ,
Helen Cooper  and
M. J. Lockyer
A. A. Holder
Affiliation:
Department of Molecular Biology, Wellcome Biotech, Langley Court, Beckenham, Kent BR3 3BS
J. S. Sandhu
Affiliation:
Department of Molecular Biology, Wellcome Biotech, Langley Court, Beckenham, Kent BR3 3BS
Yvonne Hillman
Affiliation:
Department of Molecular Biology, Wellcome Biotech, Langley Court, Beckenham, Kent BR3 3BS
Lynne S. Davey
Affiliation:
Department of Molecular Biology, Wellcome Biotech, Langley Court, Beckenham, Kent BR3 3BS
S. C. Nicholls
Affiliation:
Department of Molecular Biology, Wellcome Biotech, Langley Court, Beckenham, Kent BR3 3BS
Helen Cooper
Affiliation:
Department of Molecular Biology, Wellcome Biotech, Langley Court, Beckenham, Kent BR3 3BS
M. J. Lockyer
Affiliation:
Department of Molecular Biology, Wellcome Biotech, Langley Court, Beckenham, Kent BR3 3BS
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Summary

Specific sequences derived from the gene for the precursor to the major merozoite surface antigens (PMMSA) of Plasmodium falciparum have been expressed in Escherichia coli and the products have been used to produce antibodies. These antibodies, together with monoclonal antibodies, have been used to investigate the form of the PMMSA protein associated with merozoites. Polypeptide fragments derived by processing from the PMMSA protein have been detected in extracts of merozoites and assigned to locations within the PMMSA coding sequence.

Type
Research Article
Information
Parasitology , Volume 94 , Issue 2 , April 1987 , pp. 199 - 208
Copyright
Copyright © Cambridge University Press 1987

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