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Proteinases of Trichomonas vaginalis: antibody response in patients with urogenital trichomoniasis

Published online by Cambridge University Press:  06 April 2009

P. Bózner ,
A. Gombošová ,
M. valent ,
P. Demeš  and
J. F. Alderete
P. Bózner
Affiliation:
Institute of Microbiology and Immunology, Medical Faculty, Comenius University, Sasinkova 4, 811 08 Bratislava, Czechoslovakia
A. Gombošová
Affiliation:
Institute of Parasitology, Medical Faculty, Comenius University, Palisády 40, 811 06 Bratislava, Czechoslovakia
M. valent
Affiliation:
Institute of Parasitology, Medical Faculty, Comenius University, Palisády 40, 811 06 Bratislava, Czechoslovakia
P. Demeš
Affiliation:
Institute of Microbiology and Immunology, Medical Faculty, Comenius University, Sasinkova 4, 811 08 Bratislava, Czechoslovakia
J. F. Alderete
Affiliation:
Department of Microbiology, The University of Texas Health Science Center, San Antonio, Texas 78284–7758, USA
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Summary

Immunoprecipitation combined with electrophoresis in gelatin-polyacrylamide gels was successfully used for detection of antibodies against numerous proteinases of Trichomonas vaginalis in infected patients. The method proved to be highly specific as anti-proteinase antibodies were absent in women with negative cultivation of T. vaginalis and no history of trichomoniasis. Sera of 71% and vaginal washes of 86% patients with trichomoniasis were positive for these antibodies. In vaginal washes, but not in sera, antibodies were partly complexed with proteinases, possibly of trichomonad origin. It was also shown that serum antibodies as well as local anti-proteinase antibodies persisted for weeks after patients had been cured.

Keywords

Trichomonas vaginalistrichomoniasisproteinases
Type
Research Article
Information
Parasitology , Volume 105 , Issue 3 , December 1992 , pp. 387 - 391
Copyright
Copyright © Cambridge University Press 1992

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