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Proteomic analysis of Entamoeba histolytica

Published online by Cambridge University Press:  11 October 2006

J. TOLSTRUP ,
E. KRAUSE ,
E. TANNICH  and
I. BRUCHHAUS
J. TOLSTRUP
Affiliation:
Bernhard Nocht Institute for Tropical Medicine, 74 Bernhard Nocht Strasse, 20359 Hamburg, Germany
E. KRAUSE
Affiliation:
Leibniz Institute of Molecular Pharmacology, 10 Robert-Rössle Strasse, 13125 Berlin, Germany
E. TANNICH
Affiliation:
Bernhard Nocht Institute for Tropical Medicine, 74 Bernhard Nocht Strasse, 20359 Hamburg, Germany
I. BRUCHHAUS
Affiliation:
Bernhard Nocht Institute for Tropical Medicine, 74 Bernhard Nocht Strasse, 20359 Hamburg, Germany
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Abstract

In this study, the proteome of axenically grown Entamoeba histolytica parasites was explored by two-dimensional gel electrophoresis (2-DE), employing a practical and effective procedure for the solubilization of E. histolytica proteins. Approximately 900 protein species in the pH range between 4 and 7 were detected by Coomassie Blue staining. Ninety-five spots were excised, trypsinated and subjected to mass spectrometry. The resultant data from peptide mass fingerprints were compared with those available in the E. histolytica genome and the (non-redundant) National Center for Biotechnology Information (NCBI) databases for the identification and categorization of proteins. Sixty-three of the proteins identified were predicted to relate to the cytoskeleton, surface, glycolysis, RNA/DNA metabolism, the ubiquitin-proteasome system, vesicular trafficking and signal transduction. The present study demonstrates, for the first time, that corresponding genes are indeed expressed in E. histolytica and provides a foundation for further proteomic studies of this parasite.

Keywords

Entamoebaproteomemass spectrometry
Type
Research Article
Information
Parasitology , Volume 134 , Issue 2 , February 2007 , pp. 289 - 298
Copyright
2006 Cambridge University Press

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