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Random amplified polymorphic DNA fingerprints of the eight taxa of Trichinella and their comparison with allozyme analysis

Published online by Cambridge University Press:  06 April 2009

C. Bandi ,
G. la Rosa ,
M. G. Bardin ,
G. Damiani ,
S. Comincini ,
L. Tasciotti  and
E. Pozio
C. Bandi
Affiliation:
Istituto di Patologia Generale Veterinaria, Università di Milano, via Celoria 10, 20133 Milano, Italy
G. la Rosa
Affiliation:
Laboratory of Parasitology, Istituto Superiore di Sanità, viale Regina Elena 299, 00161 Roma, Italy
M. G. Bardin
Affiliation:
IDVGA-CNR, via Celoria 10, 20133 Milano, Italy
G. Damiani
Affiliation:
IDVGA-CNR, via Celoria 10, 20133 Milano, Italy
S. Comincini
Affiliation:
IDVGA-CNR, via Celoria 10, 20133 Milano, Italy
L. Tasciotti
Affiliation:
Laboratory of Parasitology, Istituto Superiore di Sanità, viale Regina Elena 299, 00161 Roma, Italy
E. Pozio
Affiliation:
Laboratory of Parasitology, Istituto Superiore di Sanità, viale Regina Elena 299, 00161 Roma, Italy
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Summary

Eight taxa have recently been proposed as being encompassed by the genus Trichinella on the basis of allozyme and biological data. In this paper we show that an analogous 8 taxon structure for this genus results from the random amplified polymorphic DNAs (RAPDs). Five 10-mer or 20-mer primers were used under different polymerase chain reaction (PCR) conditions to produce multiband RAPD fingerprints from muscle larvae of 40 isolates of Trichinella spp. The resulting RAPD data were analysed following the numerical taxonomic approach, and the resulting classification was compared to that derived from allozyme data. The agreement found between allozymes and RAPDs, while supporting the polyspecific structure of the genus Trichinella, confirms the potential of RAPDs as a tool for the detection of cryptic species. The selected primers were tested on individual muscle larvae in an attempt to standardize a RAPD assay for the routine identification of the 8 taxa of Trichinella. Only 1 of the 5 primers yielded reproducible fingerprints from the single larvae. Using this primer, the 5 species and the 3 other taxa of the genus Trichinella can be identified in a single assay without the need for massive in vivo parasite production.

Keywords

random amplified polymorphic DNARAPDallozymePCRTrichinellaparasite identificationtaxonomy
Type
Research Article
Information
Parasitology , Volume 110 , Issue 4 , May 1995 , pp. 401 - 407
Copyright
Copyright © Cambridge University Press 1995

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