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The rapid development of the glucose transport system in the excysted metacestode of Hymenolepis diminuta

Published online by Cambridge University Press:  06 April 2009

R. Rosen ,
M. L. San ,
M. E. Denton ,
J. M. Wolfe  and
G. L. Uglem
R. Rosen
Affiliation:
Department of Biology, Berea College, Berea, Kentucky 40404, USA
M. L. San
Affiliation:
Department of Biology, Berea College, Berea, Kentucky 40404, USA
M. E. Denton
Affiliation:
Department of Biology, Berea College, Berea, Kentucky 40404, USA
J. M. Wolfe
Affiliation:
Department of Biology, Berea College, Berea, Kentucky 40404, USA
G. L. Uglem
Affiliation:
Department of Biology, Berea College, Berea, Kentucky 40404, USA
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Summary

Temporal changes in glucose transport capacity in excysted scoleces of Hymenolepis diminuta were examined. Assays involved incubation for 1 min in [3H]glucose after pre-incubation for 1 min to 8 h in saline. There were two abrupt increases in uptake velocity, a relatively small one between 15 and 75 min, and a large one between 5 and 6 h, during which the Vmax increased from 0·36 to 2·49 nmol/25 larvae/h. The second increase was unaffected when the pre-incubation saline contained 5 mM glucose, but it was completely blocked when the excysted larvae were pre-incubated in Ca2+-free saline. Abrupt glucose transport changes did not occur in intact cysticercoids or in scoleces when the substrate was [3H]leucine or [3H]uracil. Arrhenius plots (log V versus 1/temperature, 10–42 °C) were linear for intact cysticercoids, but were biphasic for both scoleces and adults with discontinuities at 20 ± 1 °C. Thus, ‘activation’ of the excysted scolex seemed to involve a specific, Ca2+ -dependent increase in number of glucose transporters functioning in the worm surface. The Arrhenius plots indicated that development in the final host does not involve a major change in lipid composition of the parasite's membranes.

Keywords

tapewormHymenolepis diminutaglucose transport
Type
Research Article
Information
Parasitology , Volume 108 , Issue 2 , February 1994 , pp. 217 - 222
Copyright
Copyright © Cambridge University Press 1994

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