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The red fox (Vulpes vulpes) and the arctic fox (Vulpes lagopus) are definitive hosts of Sarcocystis alces and Sarcocystis hjorti from moose (Alces alces)

Published online by Cambridge University Press:  26 May 2010

STINA S. DAHLGREN  and
BJØRN GJERDE
STINA S. DAHLGREN*
Affiliation:
Norwegian School of Veterinary Science, Department of Food Safety and Infection Biology, Section of Microbiology, Immunology and Parasitology, P.O. Box 8146Dep., 0033Oslo, Norway
BJØRN GJERDE
Affiliation:
Norwegian School of Veterinary Science, Department of Food Safety and Infection Biology, Section of Microbiology, Immunology and Parasitology, P.O. Box 8146Dep., 0033Oslo, Norway
*
*Corresponding author: Tel: +47 22 96 49 68. Fax: +47 22 96 49 65. E-mail: StinaSofia.Dahlgren@nvh.no
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Summary

The aim of this study was to determine whether foxes might act as definitive hosts of Sarcocystis alces in moose. In 2 experiments, 6 silver foxes (Vulpes vulpes) and 6 blue foxes (Vulpes lagopus) were fed muscle tissue from moose containing numerous sarcocysts of S. alces, and euthanased 7–28 days post-infection (p.i.). Intestinal mucosal scrapings and faecal samples were screened microscopically for Sarcocystis oocysts/sporocysts, which were identified to species by means of species-specific primers and sequence analysis targeting the ssu rRNA gene. All foxes in both experiments became infected with Sarcocystis; the oocysts were fully sporulated by 14 days p.i., containing sporocysts measuring 14–15×10 μm. Molecular identification revealed that the oocysts/sporocysts belonged to 2 species, S. alces and Sarcocystis hjorti, although sarcocysts of S. hjorti were only identified in moose subsequent to the infection of foxes. In the first experiment, all 8 foxes also became infected with a Hammondia sp. derived from moose, shedding unsporulated, subspherical oocysts, measuring 10–12 μm in diameter, from 6–7 days p.i. onwards. The study proved that canids (the red fox and arctic fox) are definitive hosts for S. alces and S. hjorti, as had been inferred from the phylogenetic position of these species.

Keywords

HammondiaSarcocystis alcesSarcocystis hjortiVulpes lagopusVulpes vulpesssu rRNA gene
Type
Research Article
Information
Parasitology , Volume 137 , Issue 10 , September 2010 , pp. 1547 - 1557
Copyright
Copyright © Cambridge University Press 2010

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References

REFERENCES

Abel, J., Schares, G., Orzeszko, K., Gasser, R. B. and Ellis, J. T. (2006). Hammondia isolated from dogs and foxes are genetically distinct. Parasitology 132, 187–192. doi: 10.1017/S0031182005008814.CrossRefGoogle ScholarPubMed
Ashford, R. W. (1977). The fox, Vulpes vulpes, as a final host for Sarcocystis of sheep. Annals of Tropical Medicine and Parasitology 71, 29–34.CrossRefGoogle ScholarPubMed
Barta, J. R., Martin, D. S., Liberator, P. A., Dashkevicz, M., Anderson, J. W., Feighner, S. D., Elbrecht, A., Perkins-Barrow, A., Jenkins, M. C., Danforth, H. D., Ruff, M. D. and Profous-Juchelka, H. (1997). Phylogenetic relationships among eight Eimeria species infecting domestic fowl inferred using complete small subunit ribosomal DNA sequences. Journal of Parasitology 83, 262–271.CrossRefGoogle ScholarPubMed
Cheadle, M. A., Dame, J. B. and Greiner, E. C. (2001). Sporocyst size of isolates of Sarcocystis shed by the Virginia opossum (Didelphis virginiana). Veterinary Parasitology 95, 305–311. doi: 10.1016/S0304-4017(00)00396-4.CrossRefGoogle ScholarPubMed
Colwell, D. D. and Mahrt, J. L. (1983). Development of Sarcocystis alceslatrans Dubey, 1980, in the small intestine of dogs. American Journal of Veterinary Research 44, 1813–1818.Google ScholarPubMed
Dahlgren, S. S. and Gjerde, B. (2007). Genetic characterisation of six Sarcocystis species from reindeer (Rangifer tarandus tarandus) in Norway based on the small subunit rRNA gene. Veterinary Parasitology 146, 204–213. doi: 10.1016/j.vetpar.2007.02.023.CrossRefGoogle ScholarPubMed
Dahlgren, S. S. and Gjerde, B. (2008). Sarcocystis in moose (Alces alces): molecular identification and phylogeny of six Sarcocystis species in moose, and a morphological description of three new species. Parasitology Research 103, 93–110. doi: 10.1007/s00436-008-0936-1.CrossRefGoogle Scholar
Dahlgren, S. S. and Gjerde, B. (2009). Sarcocystis in Norwegian roe deer (Capreolus capreolus): molecular and morphological identification of Sarcocystis oviformis n. sp. and Sarcocystis gracilis and their phylogenetic relationship with other Sarcocystis species. Parasitology Research 104, 993–1003. doi: 10.1007/s00436-008-1281-0.CrossRefGoogle Scholar
Dahlgren, S. S. and Gjerde, B. (2010). Molecular characterization of five Sarcocystis species in red deer (Cervus elaphus), including Sarcocystis hjorti n. sp., reveals that these species are not intermediate host specific. Parasitology 137, 815–840. doi: 10.1017/S0031182009991569.CrossRefGoogle Scholar
Dahlgren, S. S., Gouveia-Oliveira, R. and Gjerde, B. (2008). Phylogenetic relationships between Sarcocystis species from reindeer and other Sarcocystidae deduced from ssu rRNA gene sequences. Veterinary Parasitology 25, 27–35. doi: 10.1016/j.vetpar.2007.09.029.CrossRefGoogle Scholar
Dubey, J. P. (1980). Sarcocystis species in moose (Alces alces), bison (Bison bison), and pronghorn (Antilocapra americana) in Montana. American Journal of Veterinary Research 41, 2063–2065.Google Scholar
Dubey, J. P., Barr, B. C., Barta, J. R., Bjerkås, I., Bjørkman, C., Blagburn, B. L., Bowman, D. D., Buxton, D., Ellis, J. T., Gottstein, B., Hemphill, A., Hill, D. E., Howe, D. K., Jenkins, M. C., Kobayashi, Y., Koudela, B., Marsh, A. E., Mattsson, J. G., McAllister, M. M., Modrý, D. , D., Omata, Y., Sibley, L. D., Speer, C. A., Trees, A. J., Uggla, A., Upton, S. J., Williams, D. J. L. and Lindsay, D. S. (2002). Redescription of Neospora caninum and its differentiation from related coccidia. International Journal for Parasitology 32, 929–946. doi: S0020-7519(02)00094-2.CrossRefGoogle ScholarPubMed
Dubey, J. P., Speer, C. A. and Fayer, R. (1989). Sarcocystosis of Animals and Man. CRC Press, Boca Raton, FL, USA.Google Scholar
Dubey, J. P. and Williams, C. S. F. (1980). Hammondia heydorni infection in sheep, goats, moose, dogs and coyotes. Parasitology 81, 123–127.CrossRefGoogle ScholarPubMed
Doležel, D., Koudela, B., Jirků, M., Hypsa, V., Oborník, M., Votýpka, J., Modrý, D., Slapeta, J. R. and Lukes, J. (1999). Phylogenetic analysis of Sarcocystis spp. of mammals and reptiles supports the coevolution of Sarcocystis spp. with their final hosts. International Journal for Parasitology 29, 795–798. doi: 10.1016/S0020-7519(99)00018-1.CrossRefGoogle ScholarPubMed
Elsheikha, H. M., Lacher, D. W. and Mansfield, L. S. (2005). Phylogenetic relationships of Sarcocystis neurona of horses and opossums to other cyst-forming coccidia deduced from ssu rRNA gene sequences. Parasitology Research 97, 345–357. doi: 10.1007/s00436-005-1396-5.CrossRefGoogle ScholarPubMed
Elsheikha, H. M., Murphy, A. J. and Mansfield, L. S. (2004). Prevalence of Sarcocystis species sporocysts in Northern Virginia opossums (Didelphis virginiana). Parasitology Research 93, 427–431. doi: 10.1007/s00436-004-1150-4.CrossRefGoogle ScholarPubMed
Entzeroth, R., Scholtyseck, E. and Greuel, E. (1978). The roe deer intermediate host of different coccidia. Naturwissenschaften 65, 395.CrossRefGoogle ScholarPubMed
Erber, M., Boch, J. and Barth, J. (1978). Drei Sarkosporidienarten des Rehwildes. Berliner und Münchener Tierärztliche Wochenschrift 91, 482–486.Google Scholar
Fenger, C. K., Granstrom, D. E., Langemeier, J. L., Stamper, S., Donahue, J. M., Patterson, J. S., Gajadhar, A. A., Marteniuk, J. V., Xiaomin, Z. and Dubey, J. P. (1995). Identification of opossums (Didelphis virginiana) as the putative definitive host of Sarcocystis neurona. Journal of Parasitology 81, 916–919.CrossRefGoogle ScholarPubMed
Fischer, S. and Odening, K. (1998). Characterization of bovine Sarcocystis species by analysis of their 18S ribosomal DNA sequences. Journal of Parasitology 84, 50–54.CrossRefGoogle ScholarPubMed
Gjerde, B. (1983). Shedding of Hammondia heydorni-like oocysts by foxes fed muscular tissue of reindeer (Rangifer tarandus). Acta Veterinaria Scandinavia 24, 241–243.CrossRefGoogle ScholarPubMed
Gjerde, B. (1985 a). The fox as a definitive host for Sarcocystis rangi from reindeer (Rangifer tarandus tarandus). Acta Veterinaria Scandinavica 26, 140–142.CrossRefGoogle Scholar
Gjerde, B. (1985 b). Ultrastructure of the cysts of Sarcocystis grueneri from cardiac muscle of reindeer (Rangifer tarandus tarandus). Zeitschrift für Parasitenkunde 71, 189–198.CrossRefGoogle ScholarPubMed
Gookin, J. L., Birkenheuer, A. J., Breitschwerdt, E. B. and Levy, M. G. (2002). Single-tube nested PCR for detection of Tritrichomonas foetus in feline feces. Journal of Clinical Microbiology 40, 4126–4130. doi: 10.1128/JCM.40.11.4126-4130.2002.CrossRefGoogle ScholarPubMed
Hernández, S., Martínez-Gómez, F., Navarrete, I. and Acosta-García, I. (1981). Estudio al microscopio optico y electronico del quiste de Sarcocystis cervicanis. Revista Ibérica de Parasitologia 41, 351–361.Google Scholar
Holmdahl, O. J., Morrison, D. A., Ellis, J. T. and Huong, L. T. (1999). Evolution of ruminant Sarcocystis (Sporozoa) parasites based on small subunit rDNA sequences. Molecular Phylogenetics and Evolution 11, 27–37. doi: 10.1006/mpev.1998.0556.CrossRefGoogle ScholarPubMed
Mohammed, O. B., Davies, A., Hussein, H. S., Daszak, P. and Ellis, J. T. (2003). Hammondia heydorni from the Arabian mountain gazelle and red fox in Saudi Arabia. Journal of Parasitology 89, 535–539. doi: 10.1645/0022-3395(2003)089.CrossRefGoogle ScholarPubMed
Schares, G., Heydorn, A. O., Cüppers, A., Mehlhorn, H., Geue, L., Peters, M. and Conraths, F. J. (2002). In contrast to dogs, red foxes (Vulpes vulpes) did not shed Neospora caninum upon feeding intermediate host tissues. Parasitology Research 88, 44–52. doi: 10.1007/s004360100496.CrossRefGoogle Scholar
Šlapeta, J. R., Modrý, D., Votýpka, J., Jirků, M., Lukes, J. and Koudela, B. (2003). Evolutionary relationships among cyst-forming coccidia Sarcocystis spp. (Alveolata: Apicomplexa: Coccidea) in endemic African tree vipers and perspective for evolution of heteroxenous life cycle. Molecular Phylogenetics and Evolution 27, 464–475. doi: 10.1016/S1055-7903(03)00018-6.CrossRefGoogle ScholarPubMed
Sedlaczek, J. and Zipper, J. (1986). Sarcocystis alceslatrans (Apicomplexa) bei einem paläarktischen Elch (Ruminantia). Angewandte Parasitologie 27, 137–144.Google ScholarPubMed
Speer, C. A. and Dubey, J. P. (1982). Sarcocystis wapiti sp. nov. from the North American wapiti (Cervus elaphus). Canadian Journal of Zoology 60, 881–888.CrossRefGoogle Scholar
Tanhauser, S. M., Yowell, C. A., Cutler, T. J., Greiner, E. C., MacKay, R. J. and Dame, J. B. (1999). Multiple DNA markers differentiate Sarcocystis neurona and Sarcocystis falcatula. Journal of Parasitology 85, 221–228. doi: 10.2307/3285623.CrossRefGoogle ScholarPubMed
Xiang, Z., Chen, X., Yang, L., He, Y., Jiang, R., Rosenthal, B. M., Luan, P., Attwood, S. W., Zuo, Y., Zhang, Y. P. and Yang, Z. (2009). Non-invasive methods for identifying oocysts of Sarcocystis spp. from definitive hosts. Parasitology International 58, 293–296. doi: 10.1016/j.parint.2009.03.004.CrossRefGoogle ScholarPubMed
Yamage, M., Flechtner, O. and Gottstein, B. (1996). Neospora caninum: specific oligonucleotide primers for the detection of brain “cysts” DNA of experimentally infected nude mice by the polymerase chain reaction (PCR). Journal of Parasitology 82, 272–279.CrossRefGoogle ScholarPubMed