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Re-Emerging foci of visceral leishmaniasis in Armenia – first molecular diagnosis of clinical samples

Published online by Cambridge University Press:  13 February 2019

Anna Sukiasyan ,
Ara Keshishyan ,
Dezdemonia Manukyan ,
Gayane Melik-Andreasyan ,
Liana Atshemyan ,
Hripsime Apresyan ,
Margarita Strelkova ,
Marcus Frohme ,
Sofia Cortes Open the ORCID record for Sofia Cortes [Opens in a new window]  and
Katrin Kuhls Open the ORCID record for Katrin Kuhls [Opens in a new window]
Anna Sukiasyan
Affiliation:
Research Institute of Epidemiology, Virology and Medical Parasitology after A.B. Alexanyan, Ministry of Health, 1 Khudyakov St., 0063, Yerevan, Armenia Division Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, Hochschulring 1, 15745 Wildau, Germany
Ara Keshishyan
Affiliation:
Research Institute of Epidemiology, Virology and Medical Parasitology after A.B. Alexanyan, Ministry of Health, 1 Khudyakov St., 0063, Yerevan, Armenia
Dezdemonia Manukyan
Affiliation:
Research Institute of Epidemiology, Virology and Medical Parasitology after A.B. Alexanyan, Ministry of Health, 1 Khudyakov St., 0063, Yerevan, Armenia
Gayane Melik-Andreasyan
Affiliation:
Research Institute of Epidemiology, Virology and Medical Parasitology after A.B. Alexanyan, Ministry of Health, 1 Khudyakov St., 0063, Yerevan, Armenia
Liana Atshemyan
Affiliation:
Research Institute of Epidemiology, Virology and Medical Parasitology after A.B. Alexanyan, Ministry of Health, 1 Khudyakov St., 0063, Yerevan, Armenia
Hripsime Apresyan
Affiliation:
Yerevan State Medical University after Mkitar Heratsi, 2 Koryun str., 0025, Yerevan, Armenia
Margarita Strelkova
Affiliation:
Martsinovsky Institute of Medical Parasitology, Tropical and Vector-Born Diseases, Sechenov First Moscow State Medical University, Malaya Pirogovskaya 20, 119830 Moscow, Russion Federation
Marcus Frohme
Affiliation:
Division Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, Hochschulring 1, 15745 Wildau, Germany
Sofia Cortes
Affiliation:
Division Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, Hochschulring 1, 15745 Wildau, Germany Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira No 100, 1349-008 Lisboa, Portugal
Katrin Kuhls*
Affiliation:
Division Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, Hochschulring 1, 15745 Wildau, Germany
*
Author for correspondence: Katrin Kuhls, E-mail: Katrin.Kuhls@th-wildau.de
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Abstract

Visceral leishmaniasis (VL) was firstly reported in Armenia in 1913. Following a considerable increase of the number of cases until the mid 1950s, the disease disappeared after 1969 and re-emerged in 1999. Scientific literature about VL in Armenia is available only in Russian or Armenian. This paper presents a historical overview about leishmaniasis in Armenia based on this literature as well as an epidemiological update since the re-emergence of the disease. In 1999–2016, 116 indigenous VL cases were recorded mainly in children in 8 of the 11 districts, however, VL is underreported because of lack of trained medical personal and diagnostic facilities. The aim of this work was to apply for the first time molecular diagnosis of VL in Armenia. Out of 25 VL suspected patients, 22 were positive by microscopy and polymerase chain reaction (PCR). Genotyping using internal transcribed spacer 1-PCR-restriction fragment length polymorphism and sequencing identified the causative agent of VL in Armenia as Leishmania infantum. The present work is an important step towards the inclusion of molecular techniques in the current diagnosis of VL in Armenia and the establishment of local molecular diagnostic facilities.

Keywords

Armeniaemerging infectious diseasesLeishmania infantummolecular diagnosismolecular typingneglected tropical diseasessouthern caucasus regionvisceral leishmaniasis
Type
Research Article
Information
Parasitology , Volume 146 , Issue 7 , June 2019 , pp. 857 - 864
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Copyright
Copyright © Cambridge University Press 2019 

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Footnotes

*

Current affiliation: Department of Biology and Pathology, Medical Faculty of the Yerevan Haybusak University, Yerevan, Armenia

†

Current affiliation: Global Health & Tropical Medicine, Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, UNL, Lisbon, Portugal

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