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Refractoriness of host haemocytes to parasite immunosuppressive factors as a putative resistance mechanism in the Biomphalaria glabrata–Echinostoma caproni system

Published online by Cambridge University Press:  12 July 2001

E. HUMBERT  and
C. COUSTAU
E. HUMBERT
Affiliation:
Centre de Biologie et d'Ecologie, UMR 5555 du CNRS, 52 Ave de Villeneuve, Université de Perpignan, 66860 Perpignan cedex, France
C. COUSTAU
Affiliation:
Centre de Biologie et d'Ecologie, UMR 5555 du CNRS, 52 Ave de Villeneuve, Université de Perpignan, 66860 Perpignan cedex, France
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Abstract

In contrast to the growing knowledge accumulated on plant resistance to pathogens, mechanisms of parasite resistance largely remain to be elucidated in animal species. In the present study we investigated mechanisms underlying resistance/susceptibility in the snail–trematode system Biomphalaria glabrata–Echinostoma caproni. In particular, we compared the effect of the parasite excretory–secretory (E–S) products on the defence functions of haemocytes from 2 susceptible and 2 resistant snail strains. In vitro experiments showed that E. caproni E–S products inhibit adhesion and phagocytosis of haemocytes from susceptible snails. A partial biochemical characterization also suggested that the interfering factor(s) is (are) heat-labile glycosylated polypeptides of molecular mass between 10 and 30 kDa. Interestingly, haemocytes from resistant snails remained unaffected by the parasite E–S products, suggesting that a constitutive difference results in their refractoriness to the parasite's immunosuppressive factor(s).

Keywords

resistanceimmunomodulationexcretory–secretory productshaemocytesBiomphalaria glabrata
Type
Research Article
Information
Parasitology , Volume 122 , Issue 6 , June 2001 , pp. 651 - 660
Copyright
© 2001 Cambridge University Press

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