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Responses of the entomopathogenic nematode, Steinernema riobrave to its insect hosts, Galleria mellonella and Tenebrio molitor

Published online by Cambridge University Press:  04 January 2007

J. M. CHRISTEN ,
J. F. CAMPBELL ,
E. E. LEWIS ,
D. I. SHAPIRO-ILAN  and
S. B. RAMASWAMY
J. M. CHRISTEN
Affiliation:
Department of Entomology, Kansas State University, Manhattan, KS 66506, USA
J. F. CAMPBELL*
Affiliation:
USDA-ARS, Grain Marketing and Production Research Center, 1515 College Ave., Manhattan, KS 66502, USA
E. E. LEWIS
Affiliation:
Department of Entomology, University of California-Davis, Davis CA 95616, USA
D. I. SHAPIRO-ILAN
Affiliation:
USDA-ARS, Southeastern Fruit and Nut Research Unit, 21 Dunbar Road, Byron, GA 31008, USA
S. B. RAMASWAMY
Affiliation:
Department of Entomology, Kansas State University, Manhattan, KS 66506, USA
*
*Corresponding author: Grain Marketing and Production Research Center, 1515 College Avenue, Manhattan, KS 66502, USA. Tel: +785 776 2717. Fax: +785 537 5584. E-mail: james.campbell@gmprc.ksu.edu
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Summary

Potential hosts for infective juveniles of entomopathogenic nematodes can vary considerably in quality based on the characteristics of the host species/stage, physiological status (e.g. stress, feeding on toxins), and infection status (heterospecific or conspecific infection). In this study, we investigated responses of the entomopathogenic nematode Steinernema riobrave to hosts (Galleria mellonella or Tenebrio molitor) that were previously parasitized with conspecifics or injected with the nematode-symbiotic bacterium, Xenorhabdus sp., to determine if there is a preference for previously parasitized/injected hosts and when this preference might occur. In no-choice bioassays, the number of juveniles infecting both host species decreased with increasing time post-infection. However, infective juveniles continued to infect previously parasitized hosts up to 72 h. Significant preference was exhibited by S. riobrave for 24 h post-infection G. mellonella larvae over uninfected, and by 24 h post-injection G. mellonella larvae over 48 h post-injection larvae. No significant preference was exhibited by S. riobrave for T. molitor hosts previously parasitized with conspecifics or those injected with bacteria in any treatment combination. Such preference for, or continued infection of parasitized insects, has the potential to impact nematode efficacy.

Keywords

Steinernema riobraveGalleria mellonellaTenebrio molitor
Type
Research Article
Information
Parasitology , Volume 134 , Issue 6 , June 2007 , pp. 889 - 898
Copyright
Copyright © Cambridge University Press 2007

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