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The RFLP analysis of the β-tubulin gene region in New World Leishmania

Published online by Cambridge University Press:  06 April 2009

A. Mendoza-León ,
J. C. Havercroft  and
D. C. Barker
A. Mendoza-León
Affiliation:
Centra de Biología Celular, Facultad de Ciencias, Universidad Central de Venezuela, Apartado 47577 Caracas 1041 A, Venezuela MRC Outstation of NIMR, Molteno Laboratories of Parasitology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, England
J. C. Havercroft
Affiliation:
MRC Outstation of NIMR, Molteno Laboratories of Parasitology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, England
D. C. Barker
Affiliation:
MRC Outstation of NIMR, Molteno Laboratories of Parasitology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, England
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Summary

We have examined the similarities and differences in the organization of tubulin genes in New World Leishmania by restriction endonuclease digestion of genomic DNA and Southern blot analysis, using heterologous and homologous tubulin gene probes. As judged by the hybridization pattern and the restriction fragment length polymorphism (RFLP), there were large differences in both the restriction and hybridization patterns of the β-tubulin sequences between stocks of the mexicana and braziliensis complexes. There were similarities in the hybridization patterns of different speciesof the mexicana complex. In contrast, a high heterogeneity was found between species of the braziliensis complex which includes intraspecific variation. The results suggest that this polymorphism may be associated with random mutations. The same analysis gave evidence of large differences in the β-tubulin gene restriction pattern between New and Old World Leishmania. This variation in the β-tubulin gene region was sufficient to distinguish between New and Old World Leishmania groups and between stocks of the mexicana and braziliensis complexes.

Keywords

LeishmaniaRLFP (Restriction Fragment Length Polymorphism)β-tubulin.
Type
Research Article
Information
Parasitology , Volume 111 , Issue 1 , July 1995 , pp. 1 - 9
Copyright
Copyright © Cambridge University Press 1995

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