Hostname: page-component-78c5997874-g7gxr Total loading time: 0 Render date: 2024-11-10T07:58:06.975Z Has data issue: false hasContentIssue false
  • English
  • Français

Ribosomal DNA sequences in the differentiation of pathogenic and non-pathogenic isolates of Entamoeba histolytica

Published online by Cambridge University Press:  06 April 2009

J. A. Cruz-Reyes ,
W. M. Spice ,
T. Rehman ,
E. Gisborne  and
J. P. Ackers
J. A. Cruz-Reyes
Affiliation:
Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT
W. M. Spice
Affiliation:
Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT
T. Rehman
Affiliation:
Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT
E. Gisborne
Affiliation:
Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT
J. P. Ackers
Affiliation:
Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT
Get access Rights & Permissions [Opens in a new window]

Summary

Recombinant ribosomal DNA sequences were amplified by PCR and used as probes to perform a fingerprint analysis of total DNA from different Entamoeba histolytica isolates. RFLPs obtained with one of the probes, R-1, support previous proposals that pathogenic and non-pathogenic E. histolytica are closely related, yet genotypically distinct. Another probe, R-2, while not distinguishing between the two forms of E. hystolytica, was able to differentiate between them and E. moshkovskii, which has morphologically identical cysts and trophozoites. A third probe, BR-1, identified strain-specific RFLPs.

Keywords

Entamoeba histolyticaribosomal genesRFLPs
Type
Research Article
Information
Parasitology , Volume 104 , Issue 2 , April 1992 , pp. 239 - 246
Copyright
Copyright © Cambridge University Press 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Albach, R., Prachayasittikul, V. & Heebner, G. M. (1984). Isolation and characterisation of RNA of Entamoeba histolytica. Molecular and Biochemical Parasitology 12, 261–72.CrossRefGoogle ScholarPubMed
Albach, R. (1989). Nucleic acids of Entamoeba histolytica. Journal of Protozoology 36, 197–205.CrossRefGoogle ScholarPubMed
Andrews, B. J., Mentzoni, L. & Bjorvatn, B. (1990). Zymodeme conversion of isolates of Entamoeba histolytica. Transactions of the Royal Society of Tropical Medicine and Hygiene 84, 63–5.CrossRefGoogle ScholarPubMed
Bhattacharya, S., Bhattacharya, A. & Diamond, L. S. (1988). Comparison of repeated DNA from strains of Entamoeba histolytica and other Entamoeba. Molecular and Biochemical Parasitology 27, 257–62.CrossRefGoogle ScholarPubMed
Bracha, R., Diamond, L. S., Ackers, J. P., Burchard, G. D. & Mirelman, D. (1990). Differentiation of clinical isolates of Entamoeba histolytica using specific DNA probes. Journal of Clinical Microbiology 28, 680–4.CrossRefGoogle ScholarPubMed
Brumpt, E. (1925). Etude sommarie de l'Entamoeba dispar nsp. Amibe a kystes quadrinuclées, parasite de l'homme. Bulletin de l'académie de Médecine, Paris 94, 943–52.Google Scholar
Burch, D. J., Reed, S., Jackson, T. F. H. G. & Stanley, S. L. Jr (1991). Isolation of a strain-specific Entamoeba histolytica cDNA clone. Journal of Clinical Microbiology 29, 696–701.CrossRefGoogle ScholarPubMed
Chomozynski, P. & Sacchi, N. (1982). Single step method for RNA isolation by guanidinium thiocyanate–phenol–chloroform extraction. Annals of Biochemistry 162, 156–9.Google Scholar
Clark, G. C. & Diamond, L. S. (1991). The Laredo strain and other Entamoeba histolytica-like amoebae are Entamoeba moshkovskii. Molecular and Biochemical Parasitology 46, 11–18.CrossRefGoogle ScholarPubMed
Cruz-Reyes, J. A. (1988). Aislamiento y caracterizacion de DNA repetitivo de Entamoeba histolytica. Tesis de Maestria. UNAM/Mexico.Google Scholar
Diamond, L. S., Harlow, D. R. & Cunnick, C. C. (1978). A new medium for the axenix cultivation of Entamoeba histolytica and other Entamoeba. Transactions of the Royal Society of Tropical Medicine and Hygiene 72, 431–2.CrossRefGoogle ScholarPubMed
Dover, G. A., Brown, S., Coen, S., Dallas, J., Strachan, T. & Trick, M. (1982). The dynamics of genome evolution and species differentiation. In Genome Evolution (ed. Dover, G. A. & Flavell, R. B.), pp. 343–72. New York: Academic Press.Google Scholar
Edman, U., Meza, I. & Agabian, N. (1987). Genomic and cDNA actin sequences from a virulent strain of Entamoeba histolytica. Proceedings of the National Academy of Sciences, USA 84, 3024–8.CrossRefGoogle ScholarPubMed
Edman, U., Meraz, M. A., Rausser, S., Agabian, N. & Meza, I. (1990). Characterisation of an immunodominant variable surface antigen from pathogenic and non-pathogenic Entamoeba histolytica. Journal of Experimental Medicine 172, 879–88.CrossRefGoogle Scholar
Esteves, M. L., Cruz-Reyes, J. A., Vargas-Villareal, J., Meraz, M. A., Perez, R., Martinez-Palomo, A., Alagon, A. & Lizardi, P. (1989). Molecular cloning and characterization of ribosomal genes from Entamoeba histolytica. XI Seminario sobre amibiasis. Summaries, p. 17.Google Scholar
Garfinkel, L. I., Giladi, M., Huber, M., Gitler, C., Mirelman, D., Ravel, M. & Rozenblatt, S. (1989). DNA probes specific for E. histolytica possessing pathogenic and non-pathogenic zymodemes. Infection and Immunity 57, 926–31.CrossRefGoogle Scholar
Huber, M., Garfinkel, L., Gitler, C., Mirelman, D., Revel, M. & Rozenblatt, S. (1987). Entamoeba histolytica: cloning and characterisation of actin cDNA. Molecular and Biochemical Parasitology 24, 227–35.CrossRefGoogle ScholarPubMed
Huber, M., Koller, B., Gitler, C., Mirelman, D., Revel, M., Rozenblatt, S. & Garfinkel, L. (1989). Entamoeba histolytica ribosomal RNA genes are carried on palindromic circular DNA molecules. Molecular and Biochemical Parasitology 32, 285–96.CrossRefGoogle ScholarPubMed
Jalan, N. K. & Maitra, T. K. (1988). Amebiasis in the developing world. In Amebiasis. Human Infection by Entamoeba histolytica (ed. Ravdin, J. I.), pp. 535–55. New York: John Wiley.Google Scholar
Jacobson, A. (1987). Purification and fractionation of Poly(A)+ RNA. Methods in Enzymology 152, 254–61.CrossRefGoogle ScholarPubMed
Martinez Palomo, A., Gonzalez Robles, A. & De La Torre, M. (1973). Selective agglutination of pathogenic strains of Entamoeba histolytica induced con A. Nature, London 245, 186–7.Google ScholarPubMed
Mirelman, D. (1987). Effect of culture conditions and bacterial associates on the zymodemes of Entamoeba histolytica. Parasitology Today 3, 37–43.CrossRefGoogle ScholarPubMed
Mirelman, D., Bracha, R., Rozenblatt, S. & Garfinkel, L. (1990). Repetitive DNA elements characteristic of pathogenic Entamoeba histolytica strains can also be detected after polymerase chain reaction in a cloned non-pathogenic strain. Infection and Immunity 58, 1660–3.CrossRefGoogle Scholar
Mirelman, D., Bracha, R., Wexler, A. & Chayen, A. (1986). Changes in isoenzyme patterns of a cloned culture of non-pathogenic Entamoeba histolytica during axenization. Infection and Immunity 54, 827–32.CrossRefGoogle Scholar
Ramirez, J. L. & Palmira, G. (1987). The ribosomal gene spacer as a tool for the taxonomy of Leishmania. Molecular and Biochemical Parasitology 22, 177–83.CrossRefGoogle ScholarPubMed
Robinson, G. L. (1968). The laboratory diagnosis of human parasitic amoebae. Transactions of the Royal Society of Tropical Medicine and Hygiene 62, 285–94.CrossRefGoogle ScholarPubMed
Sargeaunt, P. G. & Williams, J. E. (1980). A comparative study of E. histolytica, ‘E. histolytica-like’ and other morphologically identical amoebae using isoenzyme electrophoresis. Transactions of the Royal Society of Tropical Medicine and Hygiene 74, 469–74.CrossRefGoogle ScholarPubMed
Strachan, W. D., Spice, W. M., Chiodini, P. L., Moody, A. H. & Ackers, J. P. (1988). Immunological differentiation of pathogenic and non-pathogenic isolates of Entamoeba histolytica. Lancet 1, 561–3.CrossRefGoogle ScholarPubMed
Tannich, E., Horstmann, R. D., Knobloch, J. & Arnold, H. H. (1989). Genomic differences between pathogenic and non-pathogenic Entamoeba histolytica. Proceedings of the National Academy of Sciences, USA 86, 5118–22.CrossRefGoogle Scholar
Tannich, E. & Burchard, G. (1991). Differentiation of pathogenic from non-pathogenic Entamoeba histolytica by restriction fragment analysis of a single gene amplified in vitro. Journal of Clinical Microbiology 29, 250–5.CrossRefGoogle Scholar
Tannich, E., Scholze, H., Nickel, R. & Horstmann, R. D. (1991). Homologous cysteine proteinases of pathogenic and non-pathogenic Entamoeba histolytica. Journal of Biological Chemistry 266, 4798–803.CrossRefGoogle Scholar
Tobie, J. E. (1949). Experimental infection of the rabbit with Entamoeba histolytica. American Journal of Tropical Medicine and Hygiene 29, 859–70.CrossRefGoogle Scholar
Walsh, J. A. (1986). Problems in recognition and diagnosis of amoebiasis: estimation of the global magnitude of morbidity and mortality. Reviews of Infectious Diseases 8, 228–38.CrossRefGoogle ScholarPubMed