Hostname: page-component-cd9895bd7-hc48f Total loading time: 0 Render date: 2024-12-27T13:03:09.259Z Has data issue: false hasContentIssue false
  • English
  • Français

Rickettsia-like organisms and chitinase production in relation to transmission of trypanosomes by tsetse flies

Published online by Cambridge University Press:  06 April 2009

S. C. Welburn ,
K. Arnold ,
I. Maudlin  and
G. W. Gooday
S. C. Welburn
Affiliation:
Tsetse Research Laboratory, ODA/University of Bristol, Langford House, Langford, Bristol BS18 7DU
K. Arnold
Affiliation:
Department of Molecular and Cell Biology, Marischal College, University of Aberdeen, Aberdeen AB9 1AS
I. Maudlin
Affiliation:
Tsetse Research Laboratory, ODA/University of Bristol, Langford House, Langford, Bristol BS18 7DU
G. W. Gooday
Affiliation:
Department of Molecular and Cell Biology, Marischal College, University of Aberdeen, Aberdeen AB9 1AS
Get access Rights & Permissions [Opens in a new window]

Summary

Rickettsia-like organisms (RLO) from tsetse midguts and mosquito cell cultures showed high levels of endochitinase activity. A line of Glossina morsitans morsitans highly susceptible to midgut trypanosome infection and with high incidence of RLO infection showed significantly greater chitinolytic activity than G. austeni which had low RLO incidence and were correspondingly refractory to midgut infection. Midgut infection rates of Trypanosoma brucei rhodesiense in G. m. morsitans showed a dose-related increase when flies were fed N-acetyl-D-glucosamine (GlcNAc) in the infective meal and for 4 subsequent days. A model is proposed for susceptibility to trypanosome infection based on the generation of GlcNAc by RLO endochitinase activity in tsetse pupae inhibiting midgut lectin in teneral flies.

Keywords

chitinasetrypanosomiasistsetserickettsia-like organismslectins
Type
Research Article
Information
Parasitology , Volume 107 , Issue 2 , August 1993 , pp. 141 - 145
Copyright
Copyright © Cambridge University Press 1993

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Arnold, K. (1992). Chitinolytic activity in nematodes and other parasites: a possible chemotherapeutic target. Ph.D. thesis, University of Aberdeen.Google Scholar
Arnold, K., Chapell, L. & Gooday, G. W. (1992). Chitinases in Trypanosomatidae: a cautionary note. Parasitology Today 8, 273.Google Scholar
Billingsley, P. F. & Rudin, W. (1992). The role of mosquito peritrophic membrane in bloodmeal digestion and infectivity of Plasmodium species. Journal of Parasitology 78, 430–40.CrossRefGoogle ScholarPubMed
Buxton, P. A. (1955). The Natural History of Tsetse Flies. London: H. K. Lewis & Co. Ltd.Google Scholar
Cornelissen, A. W. C. A., Bakkeren, G. A. M., Barry, J. D., Michels, A. M. & Borst, P. (1985). Characteristics of trypanosome variant antigen genes active in the tsetse fly. Nucleic Acids Research 13, 4661–76.Google Scholar
Evans, D. A. & Ellis, D. S. (1983). Recent observations on the behaviour of certain trypanosomes within their insect hosts. Advances in Parasitology 22, 1–42.Google Scholar
Ham, P. J., Phiri, J. S. & Nolan, G. P. (1991). Effect of N acetyl-D-glucosamine on the migration of Brugia pahangi microfilariae into the haemocoel of Aedes aegypti. Medical and Veterinary Entomology 5, 485–93.CrossRefGoogle Scholar
Huber, M., Cabib, E. & Miller, L. H. (1991). Malaria parasite chitinase and penetration of the mosquito peritrophic membrane. Proceedings of the National Academy of Sciences 88, 2807–10.Google Scholar
Lanham, S. M. & Godfrey, D. G. (1970). Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose. Experimental Parasitology 28, 521–34.Google Scholar
Maudlin, I. & Ellis, D. S. (1985). Association between intracellular rickettsia-like infections of midgut cells and susceptibility to trypanosome infection in Glossina spp. Zeitschrift für Parasitenkunde 71, 683–7.Google Scholar
Maudlin, I. & Welburn, S. C. (1987). Lectin mediated establishment of midgut infections of Trypanosoma congolense and Trypanosoma brucei in Glossina morsitans. Tropical Medicine and Parasitology 38, 167–70.Google ScholarPubMed
Maudlin, I., Dukes, P., Luckins, A. G. & Hudson, K. M. (1986). Extrachromosomal inheritance of susceptibility to trypanosome infection in tsetse flies. II. Susceptibility of selected lines of Glossina morsitans morsitans to different stocks and species of trypanosome. Annals of Tropical Medicine and Parasitology 80, 97–105.Google Scholar
McCreath, K. J. & Gooday, G. W. (1992). A rapid and sensitive microassay for determination of chitinolytic activity. Journal of Microbiological Methods 14, 229–37.Google Scholar
Miller, N. & Lehane, M. J. (1993). Peritrophic membranes, cell surface molecules and arboparasite tropisms within arthropod vectors. Parasitology Today 9, 45–50.Google Scholar
Moloo, S. K. & Shaw, M. K. (1989). Rickettsial infections of midgut cells are not associated with susceptibility of Glossina morsitans centralis to Trypanosoma congolense infection. Acta Tropica 46, 223–7.Google Scholar
Okolo, C. J., Molyneux, D. H., Wallbanks, K. R. & Maudlin, I. (1988). Fluorescein conjugated lectins identify different carbohydrate residues on Glossina peritrophic membranes. Tropical Medicine and Parasitology 39, 208–10.Google ScholarPubMed
Schlein, Y., Raymond, L. J. & Shlomai, J. (1991). Chitinase secreted by Leishmania functions in the sandfly vector. Proceedings of the Royal Society of London, B 245, 121–6.Google Scholar
Welburn, S. C. (1991). The rickettsia-like organisms of Glossina spp. Ph.D. thesis, University of Bristol.Google Scholar
Welburn, S. C. & Gibson, W. C. (1989). Cloning of a repetitive DNA from the rickettsia-like organisms of tsetse flies (Glossina spp.). Parasitology 98, 81–4.CrossRefGoogle ScholarPubMed
Welburn, S. C. & Maudlin, I. (1987). A simple in vitro method for infecting tsetse with trypanosomes. Annals of Tropical Medicine and Parasitology 81, 453–5.Google Scholar
Welburn, S. C. & Maudlin, I. (1991). Rickettsia-like organisms, puparial temperature and susceptibility to trypanosome infection in Glossina morsitans. Parasitology 102, 201–6.Google Scholar
Welburn, S. C. & Maudlin, I. (1992). The nature of the teneral state in Glossina and its role in the acquisition of trypanosome infection in tsetse. Annals of Tropical Medicine and Parasitology 86, 529–36.Google Scholar
Welburn, S. C., Maudlin, I. & Ellis, D. S. (1987). In vitro cultivation of rickettsia-like organisms from Glossina spp. Annals of Tropical Medicine and Parasitology 81, 331–5.Google Scholar
Welburn, S. C., Maudlin, I. & ELLIS, D. S. (1989). Rate of trypanosome killing by lectins in midguts of different species and strains of Glossina. Medical and Veterinary Entomology 3, 77–82.CrossRefGoogle ScholarPubMed