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Risk of human infection with Giardia duodenalis from cats in Japan and genotyping of the isolates to assess the route of infection in cats

Published online by Cambridge University Press:  02 November 2010

J. SUZUKI ,
R. MURATA ,
S. KOBAYASHI ,
K. SADAMASU ,
A. KAI  and
T. TAKEUCHI
J. SUZUKI*
Affiliation:
Division of Clinical Microbiology, Department of Microbiology, Tokyo Metropolitan Institute of Public Health, 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan
R. MURATA
Affiliation:
Division of Clinical Microbiology, Department of Microbiology, Tokyo Metropolitan Institute of Public Health, 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan
S. KOBAYASHI
Affiliation:
Department of Tropical Medicine and Parasitology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan
K. SADAMASU
Affiliation:
Division of Clinical Microbiology, Department of Microbiology, Tokyo Metropolitan Institute of Public Health, 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan
A. KAI
Affiliation:
Division of Clinical Microbiology, Department of Microbiology, Tokyo Metropolitan Institute of Public Health, 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan
T. TAKEUCHI
Affiliation:
Department of Tropical Medicine and Parasitology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan
*
*Corresponding author: Division of Clinical Microbiology, Department of Microbiology, Tokyo Metropolitan Institute of Public Health, 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan. Tel: +81-3-3363-3231; Fax: +81-3-3368-4060; E-mail: Jun_Suzuki@member.metro.tokyo.jp
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Summary

The number of facilities in which customers make contact with cats before eating and drinking, called ‘cat cafés’, has recently increased in Tokyo, Japan. In a survey to clarify the possibility of zoonotic transmission in Giardia duodenalis, the infection rates of G. duodenalis in 321 stool samples of cats from 16 cat cafés, 31 pet shops, and the Animal Care and Consultation Center of Tokyo were 19·1% (22/115), 1·2% (1/85), and 2·5% (3/121), respectively. In the molecular analysis of 26 G. duodenalis isolates, 6 samples from 2 cat cafés belonged to the zoonotic genotype assemblage A I, and 20 other samples were of assemblage F. Moreover, phylogenetic analysis of glutamate dehydrogenase (GDH) and triosephosphate isomerase (TPI) genes of the 20 assemblage F isolates revealed 2 major lineages. The 6 assemblage A isolates belonged to the same cluster with regard to the GDH gene; however, 2 of the 6 isolates belonged to a different cluster from the other 4 isolates with regard to the TPI gene. Therefore, a risk of transmission from cats to humans is suggested because of the detection of zoonotic Giardia genotypes in cat cafés.

Keywords

Giardia duodenaliscat cafégenotypingphylogenetic analysiszoonotic disease
Type
Research Article
Information
Parasitology , Volume 138 , Issue 4 , April 2011 , pp. 493 - 500
Copyright
Copyright © Cambridge University Press 2010

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