Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-10T11:50:43.514Z Has data issue: false hasContentIssue false

Sarcocystis chloropusae (protozoa: Sarcocystidae) n. sp. from the common moorhen (Gallinula chloropus) from Egypt

Published online by Cambridge University Press:  31 March 2015

A. EL-MORSEY
Affiliation:
Parasitology and Animal Diseases Department, Veterinary Research Division, National Research Centre, 33 El Bohouth st. (former El Tahrir st.), Dokki, Giza, P.O.12622, Egypt Laboratory of Veterinary Pathology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan
M. EL-SEIFY
Affiliation:
Department of Parasitology, Faculty of Veterinary Medicine, KafrElsheikh University, KafrElsheikh, Egypt
A. Y. DESOUKY
Affiliation:
Department of Parasitology, Faculty of Veterinary Medicine, KafrElsheikh University, KafrElsheikh, Egypt
M. M. ABDEL-AZIZ
Affiliation:
Parasitology and Animal Diseases Department, Veterinary Research Division, National Research Centre, 33 El Bohouth st. (former El Tahrir st.), Dokki, Giza, P.O.12622, Egypt
H. SAKAI
Affiliation:
Laboratory of Veterinary Pathology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan
T. YANAI*
Affiliation:
Laboratory of Veterinary Pathology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan
*
* Corresponding author: Laboratory of Veterinary Pathology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501–1193, Japan. E-mail: yanai@gifu-u.ac.jp

Summary

A new name Sarcocystis chloropusae is proposed for a parasite previously found in two of 25 common moorhen (Gallinula chloropus) from Brolos Lake, Egypt. Sarcocysts were microscopic, up to 650 μm long, the cyst wall was up to 4·5 μm thick, and contained villar protrusions that were up to 4 μm long and up to 2 μm wide. The villar protrusions were crowded, contained vesicles but lacked microtubules. The ground substance layer was smooth. The bradyzoites were up to 12 μm long and up to 2 μm wide. Molecular characterization and phylogenetic analysis of the (ITS-1) supported the conclusion that the Sarcocystis in G. chloropus is a distinct species.

Type
Research Article
Copyright
Copyright © Cambridge University Press 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Dubey, J. P. and Odening, K. (2001). Toxoplasmosis and related infections. In Parasitic Diseases of Wild Mammals (ed. Samuel, W. M., Pybus, M. J. and Kocan, A. A.). pp. 478519. Iowa State University Press, Ames, IO, USA.CrossRefGoogle Scholar
Dubey, J. P., Speer, C. A. and Fayer, R. (1989). Sarcocystosis of Animals and Man. CRC Press, Boca Raton, FL, USA.Google Scholar
Edgar, R. C. (2004). MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32, 17921797.CrossRefGoogle ScholarPubMed
El-Morsey, A., El-Seify, M., Desouky, A. -R. Y., Abdel-Aziz, M. M., Sakai, H. and Yanai, T. (2014). Morphologic identification of a new Sarcocystis sp. in the common moorhen (Gallinula chloropus) (Aves: Gruiformes: Rallidae) from Brolos Lake, Egypt. Parasitology Research 113, 391397.CrossRefGoogle Scholar
Kimura, M. (1980). A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16, 111120.CrossRefGoogle ScholarPubMed
Kutkienė, L., Sruoga, A. and Butkauskas, D. (2006). Sarcocystis sp. from white-fronted goose (Anser albifrons): cyst morphology and life cycle studies. Parasitology Research 99, 562565.CrossRefGoogle ScholarPubMed
Kutkienė, L., Sruoga, A. and Butkauskas, D. (2008). Sarcocystis sp. from the goldeneye (Bucephala clangula) and the mallard (Anas platyrhynchos): cyst morphology and ribosomal DNA analysis. Parasitology Research 102, 691696.CrossRefGoogle ScholarPubMed
Kutkiene, L., Prakas, P., Sruoga, A. and Butkauskas, D. (2010). The mallard duck (Anas Platyrhynchos) as intermediate host for Sarcocystis wobeseri sp. nov. from the barnacle goose (Branta leucopsis). Parasitology Research 107, 879888.CrossRefGoogle ScholarPubMed
Kutkienė, L., Prakas, P., Sruoga, A. and Butkauskas, D. (2012). Description of Sarcocystis anasi sp. nov. and Sarcocystis albifronsi sp. nov. in birds of the order Anseriformes. Parasitology Research 110, 10431046.CrossRefGoogle ScholarPubMed
Saitou, N. and Nei, M. (1987). The neighbor joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4, 406425.Google Scholar
Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. (2013). MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30, 27252729.CrossRefGoogle ScholarPubMed