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Scanning electron microscopy of biopsy specimens removed by a colonoscope from the abomasum of sheep infected with Haemonchus contortus

Published online by Cambridge University Press:  06 April 2009

C. D. Nicholls ,
D. L. Lee  and
M. J. Sharpe
C. D. Nicholls
Affiliation:
Department of Pure and Applied Zoology, University of Leeds, Leeds LS2 9JT
D. L. Lee
Affiliation:
Department of Pure and Applied Zoology, University of Leeds, Leeds LS2 9JT
M. J. Sharpe
Affiliation:
Department of Pure and Applied Zoology, University of Leeds, Leeds LS2 9JT
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Extract

The abomasum of living sheep infected with 50000 larvae of Haemonchus contortus was examined before and during infection, by means of fibre optic endoscopy. Biopsy specimens were removed from the abomasum of the living sheep and were examined by means of scanning electron microscopy. Changes were noted in the surface structure of the abomasum 2 days after infection and larvae were seen on, and burrowing into, the mucosa. These changes became more pronounced as the infection proceeded, especially after day 10 of the infection when the adult worms had appeared. The behaviour of adult nematodes was observed within the abomasum and on occasions they were seen to move from surrounding areas into an area of haemorrhage, caused by removal of a biopsy specimen. Individual nematodes were removed from the abomasum by means of the biopsy forceps. Fibre optic endoscopy was shown to be a useful tool in the study of parasitic nematodes in vivo.

Type
Research Article
Information
Parasitology , Volume 90 , Issue 2 , April 1985 , pp. 357 - 363
Copyright
Copyright © Cambridge University Press 1985

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References

REFERENCES

Armour, J., Jarrett, W. F. H. & Jennings, F. W. (1966). Experimental Ostertagia circumcincta infections in sheep: development and pathogenesis of a single infection. American Journal of Veterinary Research 27, 1267–78.Google Scholar
Charleston, W. A. G. (1965). Pathogenesis of experimental haemonchosis in sheep, with special reference to the development of resistance. Journal of Comparative Pathology 75, 55–67.CrossRefGoogle Scholar
Christie, M. G., Brambell, M. R. & Mapes, C. J. (1967). Effect of young Haemonchus contortus on abomasal pH in sheep. Veterinary Record 80, 207–8.Google Scholar
Christie, M. G., Angus, K. W. & Hotson, I. K. (1975). Manifestations of resistance to Haemonchus contortus in sheep: worm populations and abomasal changes in sheep superinfected with 1000000 larvae of H. contortus. International Journal for Parasitology 5, 193–8.CrossRefGoogle Scholar
Hunter, A. R. & Mackenzie, G. (1982). The pathogenesis of a single challenge dose of Haemonchus contortus in lambs under six months of age. Journal of Helminthology 56, 135–44.CrossRefGoogle ScholarPubMed
Kessell, R. G. & Kardon, R. H. (1979). Tissues and Organs: a Text-atlas of Scanning Electron Microscopy. San Francisco: W. H. Freeman & Co.Google Scholar
Riddle, J. M., Rosenberg, B. F., Lucas, C. E. & Sugawa, C. (1974). The surface topography of acute erosive gastritis. In Proceedings of the Workshop on Advances in Biomedical Applications of the SEM: Scanning Electron Microscopy (Part III), pp. 769–74.Google Scholar
Ritchie, J. D. S., Anderson, N., Armour, J., Jarrett, W. F. H., Jennings, F. W. & Urquhart, G. M. (1966). Experimental Ostertagia ostertagia infection in calves: parasitology and pathogenesis of a single infection. American Journal of Veterinary Research 27, 659–67.Google Scholar
Rose, J. H. (1971). The isolation and maintenance in vivo of some gastro-intestinal nematodes and lungworms of farm animals. In Isolation and Maintenance of Parasites in vivo, Symposia of the British Society for Parasitology, vol. 9 (ed. Taylor, A. E. R. and Muller, R.), pp. 109–121. Oxford: Blackwell Scientific Publications.Google Scholar
Silverman, P. H. & Patterson, J. E. (1960). Histotrophic (parasitic) stages of Haemonchus contortus. Nature, London 185, 54–5.Google Scholar
Veglia, F. (1915). The anatomy and life history of the Haemonchus contortus. 3rd and 4th Reports of the Director of Veterinary Research, Onderstepoort. Union of South Africa, 347–500.Google Scholar