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Scanning for nucleotide variations in mitochondrial DNA fragments of Schistosoma japonicum by single-strand conformation polymorphism

Published online by Cambridge University Press:  01 January 1999

H. O. BØGH ,
X. Q. ZHU ,
B.-Z. QIAN  and
R. B. GASSER
H. O. BØGH
Affiliation:
Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia Danish Centre for Experimental Parasitology, Bülowsvej 13, DK-1870 Frederiksberg C, Denmark
X. Q. ZHU
Affiliation:
Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia
B.-Z. QIAN
Affiliation:
Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences, WHO Collaborating Centre on Helminthiasis, PR China
R. B. GASSER
Affiliation:
Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia
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Abstract

In this study, we employed a mutation scanning approach for the direct visual display of genetic variability in mitochondrial DNA (mtDNA) fragments within and among populations of Schistosoma japonicum from the People's Republic of China. Fragments of the NADH dehydrogenase 1 gene (ND1) and the cytochrome c oxidase subunit I (COI) were individually amplified from parasite DNA by polymerase chain reaction (PCR), denatured and subjected to single-strand conformation polymorphism (SSCP) analysis. Using ND1 and COI fragments, individuals representing different genotypes could be readily identified based on characteristic and reproducible SSCP profiles. The results demonstrated the usefulness of SSCP for the direct visual display of low-level sequence variation in mtDNA of S. japonicum prior to DNA sequence analysis. This approach has important implications for studying the genetic structure and biology of S. japonicum populations, and for analysing the inheritance of mitochondrial DNA.

Keywords

Schistosoma japonicummitochondrial DNANADH dehydrogenase 1cytochrome c oxidase subunit Imutations
Type
Research Article
Information
Parasitology , Volume 118 , Issue 1 , January 1999 , pp. 73 - 82
Copyright
1999 Cambridge University Press

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