Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-27T12:50:33.335Z Has data issue: false hasContentIssue false
  • English
  • Français

Schistosoma mansoni: structural damage and tegumental repair after in vivo treatment with praziquantel

Published online by Cambridge University Press:  06 April 2009

M. K. Shaw  and
D. A. Erasmus
M. K. Shaw
Affiliation:
Department of Zoology, University College, P.O. Box 78, CardiffCF1 1XL
D. A. Erasmus
Affiliation:
Department of Zoology, University College, P.O. Box 78, CardiffCF1 1XL
Get access Rights & Permissions [Opens in a new window]

Summary

The long-term, in vivo effects of a single, subcurative dose (200 mg/kg body weight of mouse) of praziquantel on the structure of adult Schistosoma mansoni and on the process and speed of tegumental repair are described. In both male and female worms praziquantel caused often extensive damage to the tegument, in the form of surface blebbings, swellings and lesions, and vacuolization and disruption of the subtegumental tissues. Repair of the drug-induced tegumental damage occurred slowly with partial and, more rarely, complete repair only being seen after 65 days post-treatment (p.t.), although signs of damage were still observed, particularly in male worms, at 100 days p.t. In contrast, repair of damage to the subtegumental/parenchymal tissues including the tegumental perikarya occurred relatively quickly, with the majority of worms examined appearing normal by 8–12 days p.t. The possible role(s) of the host immune response in relation to the speed of tegumental repair in vivo is discussed.

Type
Research Article
Information
Parasitology , Volume 94 , Issue 2 , April 1987 , pp. 243 - 254
Copyright
Copyright © Cambridge University Press 1987

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Andrews, P. (1978). Effects of praziquantel on the metabolism of Schistosoma mansoni. Fourth International Congress of Parasitology, Warsaw. Section D, 5–6.Google Scholar
Andrews, P. (1981). A summary of the efficacy of praziquantel against schistosomes in animal experiments and notes on its mode of action. Arzneimittel-Forschung/Drug Research 31, 538–41.Google ScholarPubMed
Andrews, P., Thomas, H. & Weber, H. (1980). The in vitro uptake of 14C-praziquantel by cestodes, trematodes and a nematode. Journal of Parasitology 66, 920–5.CrossRefGoogle Scholar
Becker, B., Mehlhorn, H., Andrews, P., Thomas, H. & Eckert, J. (1980). Light and electron microscopic studies on the effects of praziquantel on Schistosoma mansoni, Dicrocoelium dendriticum, and Fasciola hepatica (Trematoda) in vitro. Zeitschrift für Parasitenkunde 63, 113–28.CrossRefGoogle ScholarPubMed
Bentley, A. G. (1982). Tegumental repair in the adult trematode Ochetosoma aniarum. Journal of Parasitology 68, 1096–1104.CrossRefGoogle Scholar
Carlisle, S., Weisberg, L. S. & Bentley, A. G. (1983). Schistosoma mansoni: Morphologic changes induced by maintenance in vitro. Journal of Parasitology 69, 319–34.CrossRefGoogle ScholarPubMed
Coles, G. C. (1984). Recent advances in schistosome biochemistry. Parasitology 89, 603–37.CrossRefGoogle ScholarPubMed
Doenhoff, M. J. & Bain, J. (1978). The immune-dependence of schistosomicidal chemotherapy: Relative lack of efficacy of an antimonal in Schistosoma mansoni-infected mice deprived of their T-cells and the demonstration of drug-antiserum synergy. Clinical and Experimental Immunology 33, 232–8.Google Scholar
Duvall, R. H. & Dewitt, W. B. (1967). An improved perfusion technique for recovering adult schistosomes from laboratory animals. American Journal of Tropical Medicine and Hygiene 16, 483–6.CrossRefGoogle ScholarPubMed
Hockley, D. J. (1973). Ultrastructure of the tegument of Schistosoma. Advances in Parasitology 11, 233–305.CrossRefGoogle ScholarPubMed
Kusel, J. R. & Mackenzie, P. E. (1975). The measurement of the relative turnover rates of proteins of the surface membranes and other fractions of Schistosoma mansoni in culture. Parasitology 71, 261–73.CrossRefGoogle ScholarPubMed
Leitch, B. & Probert, A. J. (1984). Schistosoma haematobium: Amoscanate and adult worm ultrastructure. Experimental Parasitology 58, 278–89.CrossRefGoogle ScholarPubMed
McLaren, D. J. (1980). Schistosoma mansoni: The parasite surface in relation to host immunity. In Tropical Medicine Research Studies 1. Chichester: Research Studies Press, John Wiley Press.Google Scholar
Mehlhorn, H., Becker, B., Andrews, P., Thomas, H. & Frenkel, J. K. (1981). In vivo and in vitro experiments on the effects of praziquantel on Schistosoma mansoni. Arzneimittel-Forschung/Drug Research 31, 544–54.Google ScholarPubMed
Mehlhorn, H., Frenkel, J. K., Andrews, P. & Thomas, H. (1982). Light and electron microscopic studies on Schistosoma mansoni granulomas of mice livers following treatment with praziquantel. Tropenmedizin und Parasitologie 33, 229–39.Google ScholarPubMed
Perez, H. & Terry, R. J. (1973). The killing of adult Schistosoma mansoni in vitro in the presence of antisera to host antigenic determinants and peritoneal cells. International Journal for Parasitology 3, 499–503.CrossRefGoogle ScholarPubMed
Philipp, M. & Rumjanek, F. D. (1984). Antigenic and dynamic properties of helminth surface structures. Molecular and Biochemical Parasitology 10, 245–68.CrossRefGoogle ScholarPubMed
Popiel, I. & Erasmus, D. A. (1984). Schistosoma mansoni: Ultrastructure of adults from mice treated with oxamniquine. Experimental Parasitology 58, 254–62.CrossRefGoogle ScholarPubMed
Popiel, I., Irving, D. L. & Basch, P. F. (1985). Wound healing in the trematode Schistosoma. Tissue and Cell 17, 69–77.CrossRefGoogle ScholarPubMed
Sabah, A. A., Fletcher, C., Webbe, G. & Doenhoff, M. J. (1985). Schistosoma mansoni: Reduced efficacy of chemotherapy in infected T-cell-deprived mice. Experimental Parasitology 60, 348–54.CrossRefGoogle ScholarPubMed
Shaw, M. K. & Erasmus, D. A. (1983 a). Schistosoma mansoni: The effects of a subcurative dose of praziquantel on the ultrastructure of worms in vivo. Zeitschrift für Parasitenkunde 69, 73–90.CrossRefGoogle ScholarPubMed
Shaw, M. K. & Erasmus, D. A. (1983 b). Schistosoma mansoni: Dose-related tegumental surface changes after in vivo treatment with praziquantel. Zeitschrift für Parasitenkunde 69, 643–53.CrossRefGoogle ScholarPubMed
Simpson, A. J. G. & McLaren, D. J. (1982). Schistosoma mansoni: Tegumental damage as a consequence of lectin binding. Experimental Parasitology 53, 105–16.CrossRefGoogle ScholarPubMed
Smithers, S. R. & Terry, R. J. (1965). Infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of adult worms. Parasitology 55, 695–700.CrossRefGoogle ScholarPubMed
Standen, O. D. (1962). Observations in mice on the schistosomicidal properties of 1,7–bis (p–aminophenoxy) heptane in vivo and in vivo/in vitro. Ciba Foundation Symposium: Bilharziasis (ed. Wolstenholme, G. E. W. and M., O'Connor), London: pp. 266–86. J. & A. Churchill Ltd.CrossRefGoogle Scholar
Voge, M. & Bueding, E. (1980). Schistosoma mansoni: Tegumental surface alterations induced by subcurative doses of the schistosomicide amoscanate. Experimental Parasitology 50, 251–9.CrossRefGoogle ScholarPubMed
Wilson, R. A. & Barnes, P. E. (1974). An in vitro investigation of dynamic processes occurring in the schistosome tegument, using compounds known to disrupt secretory processes. Parasitology 68, 259–70.CrossRefGoogle Scholar
Wilson, R. A. & Barnes, P. F. (1977). The formation and turnover of the membranocalyx of the tegument of Schistosoma mansoni. Parasitology 74, 61–71.CrossRefGoogle ScholarPubMed