Hostname: page-component-cd9895bd7-dk4vv Total loading time: 0 Render date: 2024-12-27T09:19:48.081Z Has data issue: false hasContentIssue false
  • English
  • Français

Sequential exposure and assembly of cyst wall filaments on the surface of encysting Giardia duodenalis

Published online by Cambridge University Press:  16 January 2003

R. ARGUELLO-GARCIA ,
C. ARGUELLO-LOPEZ ,
A. GONZALEZ-ROBLES ,
A. M. CASTILLO-FIGUEROA  and
M. G. ORTEGA-PIERRES
R. ARGUELLO-GARCIA
Affiliation:
Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados-IPN, 07300 Mexico City, D.F., Mexico Department of Experimental Pathology, Centro de Investigación y de Estudios Avanzados-IPN, 07300 Mexico City, D.F., Mexico
C. ARGUELLO-LOPEZ
Affiliation:
Department of Experimental Pathology, Centro de Investigación y de Estudios Avanzados-IPN, 07300 Mexico City, D.F., Mexico
A. GONZALEZ-ROBLES
Affiliation:
Department of Experimental Pathology, Centro de Investigación y de Estudios Avanzados-IPN, 07300 Mexico City, D.F., Mexico
A. M. CASTILLO-FIGUEROA
Affiliation:
Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados-IPN, 07300 Mexico City, D.F., Mexico
M. G. ORTEGA-PIERRES
Affiliation:
Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados-IPN, 07300 Mexico City, D.F., Mexico
Get access Rights & Permissions [Opens in a new window]

Abstract

The mode of appearance and assembly of cyst wall filaments on the surface of Giardia duodenalis trophozoites committed to encyst was analysed by scanning and transmission electron microscopy (SEM and TEM) and by fluorescence microscopy (FM). SEM showed a progressive appearance of fibril patches, predominantly on the anterior area of ventral and dorsal surfaces, which then spread and coalesced. By TEM, ruthenium red (RR) displayed staining in encysting cells as rodlike spots of variable diameter (3–25 nm), possibly microfibril tips with polyanionic moieties, that displayed tangential associations and random orientations over the cell membrane. In FM assays, the 1,10-phenanthroline derivative of ruthenium red (RR/oPHE) was a specific ligand for these assembling fibrils and this staining was significantly blocked by N-acetylgalactosamine (GalNac) and galactosamine (GalN). Interestingly, RR staining was lost when the cyst wall was completely assembled and thickened as observed by TEM and FM. Kinetic FM assays, in which a mAb specific for a 26 kDa Giardia cyst wall polypeptide was used concomitantly with RR/oPHE staining, showed a differential pattern for the appearance and reactivity of polypeptide and assembling GalN/GalNac-rich moieties of Giardia cyst wall.

Keywords

Giardia duodenalisencystationcyst wall assemblyelectron microscopyfluorescence microscopy
Type
Research Article
Information
Parasitology , Volume 125 , Issue 3 , September 2002 , pp. 209 - 219
Copyright
© 2002 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)