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Simple and reliable preparation of immunodiagnostic antigens for Taenia solium cysticercosis

Published online by Cambridge University Press:  21 June 2013

YASUHITO SAKO ,
SONOYO ITOH ,
MUNEHIRO OKAMOTO ,
KAZUHIRO NAKAYA  and
AKIRA ITO
YASUHITO SAKO*
Affiliation:
Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido, Japan
SONOYO ITOH
Affiliation:
Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido, Japan
MUNEHIRO OKAMOTO
Affiliation:
Center for Human Evolution Modeling Research, Primate Research Institute, Kyoto University, Inuyama, Aichi, Japan
KAZUHIRO NAKAYA
Affiliation:
Animal Laboratory for Medical Research, Center for Advanced Research and Education, Asahikawa Medical University, Asahikawa, Hokkaido, Japan
AKIRA ITO
Affiliation:
Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido, Japan
*
*Corresponding author: Department of Parasitology, Asahikawa Medical University, Midorigaoka Higashi 2-1-1-1, Asahikawa, 078-8510 Hokkaido, Japan. Tel: +81-166-2422. Fax: +81-166-68-2429. E-mail: yasusako@asahikawa-med.ac.jp
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Summary

Cysticercosis caused by infection with the larval stage of Taenia solium is an important cause of neurological disease worldwide and immunodiagnosis is important for the control and elimination of cysticercosis. In the present study, we established a simple and reliable preparation of immunodiagnostic low-molecular-weight antigens (LMWAgs) from T. solium cyst fluids by a cation-exchange chromatography (CEC). Banding patterns of LMWAgs on SDS-PAGE were different between isolates from Ecuador and China. All cysticercosis patient sera and some echinococcosis patient sera recognized both LMWAgs by enzyme-linked immunosorbent assay (ELISA), but sera from healthy persons were not positive. There was no statistical difference in immunodiagnostic performance of LMWAgs prepared from different geographical isolates. These results indicated that these novel immunodiagnostic antigen preparations could contribute the control and prevention of cysticercosis in endemic areas, especially developing countries.

Keywords

Taenia soliumcysticercosisimmunodiagnostic antigenslow-molecular-weight antigenscation-exchange chromatographysimple preparation
Type
Research Article
Information
Parasitology , Volume 140 , Special Issue 13: Control of cestode zoonoses in Asia: role of basic and applied science , November 2013 , pp. 1589 - 1594
Copyright
Copyright © Cambridge University Press 2013 

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