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A simple staining technique to demonstrate the sporozoite refractile globule in coccidian parasites

Published online by Cambridge University Press:  06 April 2009

M. A. Peirce
M. A. Peirce
Affiliation:
Department of Parasitology, Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, New Haw, Weybridge, Surrey, KT15 3NB
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Extract

A staining technique was developed for locating the sporozoites of Eimeria spp. in both mammalian and avian hosts. Bouin-fixed tissues embedded in Paraffin wax and cut at a thickness of 3 μg were stained by a controlled sequence of Ehrlich's haematoxylin, acid fuchsin and aniline blue–orange G–acetic acid which took only 30 min to perform. The refractile globule of the sporozoite stained a brilliant crimson whilst the tissue had an overall blue colour, thus enabling rapid scanning of material under low-power magnification. The granules of eosinophils and heterophils also stained a brilliant crimson and their significance in immunological responses indicates a wider application for this technique.

Type
Research Article
Information
Parasitology , Volume 80 , Issue 3 , June 1980 , pp. 551 - 554
Copyright
Copyright © Cambridge University Press 1980

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References

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