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Single nucleotide variation in rDNA ITS-2 differentiates Psoroptes isolates from sheep and rabbits from the same geographical area

Published online by Cambridge University Press:  01 October 1999

H. OCHS ,
A. MATHIS  and
P. DEPLAZES
H. OCHS
Affiliation:
Institute of Parasitology, University of Zurich, Winterthurerstrasse 266a, CH-8057 Zurich, Switzerland
A. MATHIS
Affiliation:
Institute of Parasitology, University of Zurich, Winterthurerstrasse 266a, CH-8057 Zurich, Switzerland
P. DEPLAZES
Affiliation:
Institute of Parasitology, University of Zurich, Winterthurerstrasse 266a, CH-8057 Zurich, Switzerland
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Abstract

Psoroptic sheep scab and psoroptic otoacariasis of domestic rabbits occur in the same geographical regions of Switzerland. To address the question as to whether Psoroptes mites are naturally transmitted between sheep and rabbits, we determined the sequences of the rDNA second internal transcribed spacer (ITS-2) from psoroptic mites from sheep (5 field isolates from Switzerland and 2 laboratory isolates from Ireland and the UK) and from rabbits (8 field isolates from Switzerland). The ITS-2 sequences for all Psoroptes mites originating from sheep were identical and differed at 1 nucleotide position from all the sequences of the rabbit-derived isolates. A statistically significant difference between a rabbit-derived isolate and isolates originating from sheep was also obtained by morphometric analysis of the lengths of the outer opisthosomal setae. For comparative purposes, the ITS-2 sequences from Chorioptes and Sarcoptes collected in Switzerland were also determined. No intraspecies variation was found in 6 sarcoptic isolates from red foxes, with a sequence identity of 41% as compared to Psoroptes. The ITS-2 sequences of 3 chorioptic isolates differed by 24–29% from the Psoroptes sequence. Identical sequences were found for the Chorioptes isolates from sheep and a camel, which differed by 18% from the sequence of an isolate from a cow. These genetic data of psoroptic mites originating from sheep and rabbits from the same geographical area suggest the existence of epidemiologically separated populations.

Keywords

epidemiologyPsoroptesChorioptesSarcoptesITS-2morphology
Type
Research Article
Information
Parasitology , Volume 119 , Issue 4 , October 1999 , pp. 419 - 424
Copyright
1999 Cambridge University Press

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