Hostname: page-component-cd9895bd7-8ctnn Total loading time: 0 Render date: 2024-12-28T03:38:14.828Z Has data issue: false hasContentIssue false
  • English
  • Français

Sodium azide preservation of faecal specimens for Kato analysis

Published online by Cambridge University Press:  06 April 2009

D. A. P. Bundy ,
J. D. M. Foreman  and
M. H. N. Golden
D. A. P. Bundy
Affiliation:
Department of Zoology, University of the West Indies, Kingston 7, Jamaica
J. D. M. Foreman
Affiliation:
Wellcome Trace Element Research Group, Tropical Metabolism Research Unit, Kingston 7, Jamaica
M. H. N. Golden*
Affiliation:
Wellcome Trace Element Research Group, Tropical Metabolism Research Unit, Kingston 7, Jamaica
*
*Author for correspondence.
Get access Rights & Permissions [Opens in a new window]

Extract

The modified Kato technique has the advantages of reproducibility, simplicity and economy: the disadvantage is that it cannot be used in conjunction with traditional faecal preservatives. Sodium azide has been evaluated as a preservative for human faeces for subsequent Kato analysis. More than 400 faecal samples (from normal and malnourished children, and from mixed-age participants in a field survey of the Turks and Caicos Islands) were each mixed with 2–5 mg of sodium azide powder and stored in 2 or 4 ml autoanalyser cups at ambient tropical temperature. At intervals up to 30 weeks, aliquots were prepared for Kato analysis. Trichuris trichiura, Ascaris lumbricoides and Necator americanus eggs were well preserved without degenerative or developmental changes in morphology. Quantitative analyses of 18 samples indicated that the mean egg count/sample did not change significantly after storage for 1, 2, 4, 8, 12 and 16 weeks in preservative. The use of azide preservative extends the applications of the Kato technique to field and clinical studies in which delays may occur between specimen collection and examination. The direct costs of azide preservation are substantially lower than for traditional methods and the preserved specimens are more compact and resistant to leakage.

Type
Research Article
Information
Parasitology , Volume 90 , Issue 3 , June 1985 , pp. 463 - 469
Copyright
Copyright © Cambridge University Press 1985

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Baltsheffsky, H. & Baltsheffsky, M. (1974). Electron transport phosphorylation. Annual Reviews of Biochemistry 43, 871–83.CrossRefGoogle Scholar
Boros, D. L. & Warren, K. S. (1970). Delayed hypersensitivity type granuloma formation and dermal reaction induced and elicited by a soluble factor isolated from Schistosoma mansoni eggs. Journal of Experimental Medicine 132, 488–507.CrossRefGoogle ScholarPubMed
Bretherick, L. (1975). Handbook of Reactive Chemical Hazards. London: Butterworth and Co.Google Scholar
Brock, T. D. (1974). Biology of Microorganisms. New Jersey: Prentice Hall.Google Scholar
Efthymiou, C. J. & Joseph, S. W. (1974). Development of a selective enterococcus medium based on manganese ion deficiency, sodium azide and alkaline pH. Applied Microbiology 28, 411–16.CrossRefGoogle ScholarPubMed
Facklam, R. & Wilkinson, H. W. (1981). The Family Streptococceae (Medical Aspects). In The Prokaryotes, vol. 3 (ed. Starr, E., Stolp, H., Truper, H. G., Balows, A. and Schlegel, H. G.). Berlin: Springer-Verlag.Google Scholar
Jordan, P., Bartholomew, R. K. & Peters, P. A. S. (1981). A community study of Schistosoma mansoni egg excretion assessed by the Bell and modified Kato technique. Annals of Tropical Medicine and Parasitology 75, 35–40.CrossRefGoogle ScholarPubMed
Kato, K. & Miura, M. (1954). Comparative examinations. Japanese Journal of Parasitology 3, 35. (Japanese text.)Google Scholar
Katz, N., Marcos, P., Coelho, Z. & Pellegrino, J. (1970). Evaluation of Kato's quantitative method through the recovery of Schistosoma mansoni eggs added to human faeces. Journal of Parasitology 56, 1032–3.CrossRefGoogle Scholar
Knight, W. B., Hiatt, R. A., Cline, B. L. & Ritchie, L. S. (1976). A modification of the formol-ether concentration techniques for increased sensitivity in detecting Schistosoma mansoni eggs. American Journal of Tropical Medicine and Hygiene 25, 818–23.CrossRefGoogle ScholarPubMed
Martin, L. K. & Beaver, P. C. (1968). Evaluation of Kato's thick smear technique for quantitative diagnosis of helminth infections. American Journal of Tropical Medicine and Hygiene 17, 382–91.CrossRefGoogle Scholar
Reinhold, G. W., Swern, M. & Hussong, R. V. (1953). A plating medium for the isolation and enumeration of enterococci. Journal of Dairy Science 36, 1–6.CrossRefGoogle Scholar
Sax, N. I. (1975). Dangerous Properties of Industrial Materials, 4th ed.New York: Van Nostrand Reinhold Co.Google Scholar
Snyder, M. L. & Lichstein, J. C. (1940). Sodium azide as an inhibiting substance for Gram-negative bacteria. Journal of Infectious Diseases 67, 113–15.CrossRefGoogle Scholar
Teesdale, C. H. & Amin, M. A. (1976). Comparison of the Bell Technique, a modified Kato thick smear technique, and a digestion method for the field diagnosis of Schistosomiasis mansoni. Journal of Helminthology 50, 17–20.CrossRefGoogle Scholar
Warren, K. S., Mahmoud, A. A. F., Cummings, P., Murphy, D. J. & Houser, H. B. (1974). Schistosomiasis mansoni in Yemeni in California. Duration of infection, presence of disease, and therapeautic management. American Journal of Tropical Medicine and Hygiene 23, 902–9.CrossRefGoogle Scholar