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Species diversity of dermal microfilariae of the genus Cercopithifilaria infesting dogs in the Mediterranean region

Published online by Cambridge University Press:  23 August 2012

DOMENICO OTRANTO ,
EMANUELE BRIANTI ,
FILIPE DANTAS-TORRES ,
GUADALUPE MIRÓ ,
MARIA STEFANIA LATROFA ,
YASEN MUTAFCHIEV  and
ODILE BAIN
DOMENICO OTRANTO*
Affiliation:
Dipartimento di Sanità Pubblica e Zootecnia, Facoltà di Medicina Veterinaria, Università degli Studi di Bari, Valenzano, Bari, Italy
EMANUELE BRIANTI
Affiliation:
Dipartimento di Sanità Pubblica Veterinaria, Facoltà di Medicina Veterinaria, Università degli Studi di Messina, Messina, Italy
FILIPE DANTAS-TORRES
Affiliation:
Dipartimento di Sanità Pubblica e Zootecnia, Facoltà di Medicina Veterinaria, Università degli Studi di Bari, Valenzano, Bari, Italy Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães (Fiocruz-PE), Recife, Pernambuco, Brazil
GUADALUPE MIRÓ
Affiliation:
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, de Madrid, Spain
MARIA STEFANIA LATROFA
Affiliation:
Dipartimento di Sanità Pubblica e Zootecnia, Facoltà di Medicina Veterinaria, Università degli Studi di Bari, Valenzano, Bari, Italy
YASEN MUTAFCHIEV
Affiliation:
Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Sofia, Bulgaria
ODILE BAIN
Affiliation:
Département Systématique et Evolution, UMR 7205 CNRS, Muséum National d'Histoire Naturelle, Paris, France
*
*Corresponding author: Dipartimento di Sanità Pubblica e Zootecnia, Facoltà di Medicina Veterinaria, Università degli Studi di Bari, Valenzano, Bari, Italy. Tel/Fax: +39 080 4679839. E-mail: domenico.otranto@uniba.it
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Summary

Following the recent description of microfilariae of a Cercopithifilaria sp. in a dog from Sicily, Italy, (herein after referred to as Cercopithifilaria sp. I), numerous skin samples were collected from dogs in the Mediterranean region. In addition to Cercopithifilaria sp. I (185·7 ± 7·2 μm long), microfilariae of 2 other species were identified, namely Cercopithifilaria grassii (651·7 ± 23·6 μm long) and a yet undescribed microfilaria, Cercopithifilaria sp. II (264·4 ± 20·2 μm long, with evident lateral alae). The morphological differentiation among the 3 species of dermal microfilariae was confirmed by differences in cytochrome c oxidase subunit 1 and ribosomal 12S sequences examined (mean level of interspecific pairwise distance of 11·4%, and 17·7%, respectively). Phylogenetic analyses were concordant in clustering these with other sequences of Cercopithifilaria spp. to the exclusion of Dirofilaria spp., Onchocerca spp. and Acanthocheilonema spp. Dermal microfilariae collected (n = 132) were morphologically identified as Cercopithifilaria sp. I (n = 108, 81·8%), Cercopithifilaria sp. II (n = 17, 12·9%), whereas only 7 (5·3%) were identified as C. grassii. Mixed infestations were detected in all sites examined. The great diversity of these neglected filarioids in dogs is of biological interest, considering the complex interactions occurring among hosts, ticks and Cercopithifilaria spp. in different environments.

Keywords

Cercopithifilaria spp.Cercopithifilaria grassiidogbiodiversitydermal microfilariaehistology
Type
Research Article
Information
Parasitology , Volume 140 , Issue 1 , January 2013 , pp. 99 - 108
Copyright
Copyright © Cambridge University Press 2012

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