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A species-specific oligonucleotide DNA probe for the identification of Meloidogyne incognita

Published online by Cambridge University Press:  06 April 2009

M. R. Chacon ,
R. M. E. Parkhouse ,
M. P. Robinson ,
P. R. Burrows  and
T. Garate
M. R. Chacon
Affiliation:
Division of Immunology, National Institute for Medical Research, Mill Hill, London NW7 1AA, UK Centro Nacional de Biotecnologia, C, Serrano 115, Madrid, Spain
R. M. E. Parkhouse
Affiliation:
Division of Immunology, National Institute for Medical Research, Mill Hill, London NW7 1AA, UK
M. P. Robinson
Affiliation:
Entomology and Nematology Department, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts AL5 2JQ, UK
P. R. Burrows
Affiliation:
Entomology and Nematology Department, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts AL5 2JQ, UK
T. Garate
Affiliation:
Division of Immunology, National Institute for Medical Research, Mill Hill, London NW7 1AA, UK Centro Nacional de Biotecnologia, C, Serrano 115, Madrid, Spain
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Abstract

A genomic library of Meloidogyne incognita Race 1 has been prepared in the bacteriophage λgt10 and screened for specific DNA sequences by hybridization with radio-isotope labelled total genomic DNA from a number of Meloidogyne species. One clone isolated (MR1#15), although not totally species specific, clearly showed preferential hybridization to M. incognita. Following subcloning and sequencing of the 255 bp insert, four stretches of the sequence corresponding to oligonucleotides of approximately equal length (~60 bp) were synthesized and examined for specificity. One of them, MR1#15.2, showed the necessary specificity to be used as a diagnostic tool.

Keywords

MeloidogyneoligonucleotideDNA probediagnosis
Type
Research Article
Information
Parasitology , Volume 103 , Issue 2 , October 1991 , pp. 315 - 319
Copyright
Copyright © Cambridge University Press 1991

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