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A species-specific oligonucleotide DNA probe for the identification of Meloidogyne incognita

Published online by Cambridge University Press:  06 April 2009

M. R. Chacon ,
R. M. E. Parkhouse ,
M. P. Robinson ,
P. R. Burrows  and
T. Garate
M. R. Chacon
Affiliation:
Division of Immunology, National Institute for Medical Research, Mill Hill, London NW7 1AA, UK Centro Nacional de Biotecnologia, C, Serrano 115, Madrid, Spain
R. M. E. Parkhouse
Affiliation:
Division of Immunology, National Institute for Medical Research, Mill Hill, London NW7 1AA, UK
M. P. Robinson
Affiliation:
Entomology and Nematology Department, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts AL5 2JQ, UK
P. R. Burrows
Affiliation:
Entomology and Nematology Department, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts AL5 2JQ, UK
T. Garate
Affiliation:
Division of Immunology, National Institute for Medical Research, Mill Hill, London NW7 1AA, UK Centro Nacional de Biotecnologia, C, Serrano 115, Madrid, Spain
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Abstract

A genomic library of Meloidogyne incognita Race 1 has been prepared in the bacteriophage λgt10 and screened for specific DNA sequences by hybridization with radio-isotope labelled total genomic DNA from a number of Meloidogyne species. One clone isolated (MR1#15), although not totally species specific, clearly showed preferential hybridization to M. incognita. Following subcloning and sequencing of the 255 bp insert, four stretches of the sequence corresponding to oligonucleotides of approximately equal length (~60 bp) were synthesized and examined for specificity. One of them, MR1#15.2, showed the necessary specificity to be used as a diagnostic tool.

Keywords

MeloidogyneoligonucleotideDNA probediagnosis
Type
Research Article
Information
Parasitology , Volume 103 , Issue 2 , October 1991 , pp. 315 - 319
Copyright
Copyright © Cambridge University Press 1991

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References

Barker, D. C. (1989). Molecular approaches to DNA diagnosis. Parasitology 99, S125–S146.CrossRefGoogle ScholarPubMed
Burrows, P. R. (1990). The rapid and sensitive detection of the plant parasitic nematode Globodera pallida using a non-radioactive biotinylated DNA probe. Revue de Nematologie 13, 185–90.Google Scholar
Burrows, P. R. & Perry, R. N. (1988). Two cloned DNA fragments which differentiate Globodera pallida from G. rostochiensis. Revue de Nematologie 90, 137–44.Google Scholar
Curran, J., Baillie, D. L. & Webster, J. M. (1985). Use of genomic DNA restriction fragment length differences to identify nematode species. Parasitology 90, 137–44.Google Scholar
Curran, J., McClure, M. A. & Webster, J. M. (1986). Genotypic differentiation of Meloidogyne population by detection of restriction fragment length differences in total DNA. Journal of Nematology 18, 83–6.Google ScholarPubMed
Denhardt, D. T. (1966). A membrane filter technique for the detection of complementary DNA. Biochemical and Biophysical Research Communications 23, 641–6.Google Scholar
Essex, R. P., Perry, V. G. & Taylor, A. L. (1976). A diagnostic compendium of the genus Meloidogyne (Nematodes: Heteroderae). Proceedings of the Helminthological Society, Washington 43, 138–50.Google Scholar
Feinberg, A. P. & Volgestein, B. (1983). A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 132, 6–13.Google Scholar
Feinberg, A. P. & Volgestein, B. (1984). Addendum ‘A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity’. Analytical Biochemistry 137, 266–57.Google ScholarPubMed
Garate, T., Harnett, W. & Parkhouse, R. M. E. (1990). Cloning of a species-specific DNA probe for Onchocerca gibsoni. International Journal for Parasitology 20, 31–5.CrossRefGoogle ScholarPubMed
Harnett, W., Chambers, A. E., Renz, A. & Parkhouse, R. M. E. (1989). An oligonucleotide probe specific for Onchocerca volvulus. Molecular and Biochemical Parasitology 35, 119–26.CrossRefGoogle ScholarPubMed
Hussey, R. S. (1979). Biochemical systematics of nematodes: a review. Helminthological Abstracts, Series B, Plant Nematology 48, 141–8.Google Scholar
Hussey, R. S. (1990). Biochemical and molecular methods of identifying Meloidogyne species. Journal of Nematology 22, 8–9.Google ScholarPubMed
Hyman, B. C., Peloquin, J. J. & Platzer, E. G. (1990). Optimization of mitocondrial DNA-based hybridisation assays to diagnostic in soil. Journal of Nematology 22, 273–8.Google Scholar
Maniatis, L., Fritsch, E. F. & Sambrook, J. (1982). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory.Google Scholar
Maxam, A. M. & Gilbert, W. (1980). Sequencing end-labelled DNA with base-specific chemical cleaves. Methods in Enzymology 65, 499–560.Google Scholar
McClure, M. A., Kruk, T. J. & Misaglu, I. (1973). A method of obtaining quantities of clean Meloidogyne eggs. Journal of Nematology 6, 17–20.Google Scholar
Powers, T. O., Platzer, E. G. & Hyman, B. C. (1986). Species-specific restriction site polymorphism in root-knot nematode mitochondrial DNA. Journal of Nematology 18, 288–96.Google ScholarPubMed
Rigby, P. W. J., Dickman, M., Rhode, S. C. & Berg, P. (1977). Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase 1. Journal of Molecular Biology 113, 237–51.Google Scholar
Sanger, F., Nicklen, S. & Coulson, R. (1977). DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA. 74, 5463–7.Google Scholar
Sasser, J. N. (1952). Identification of root-knot nematodes (Meloidogyne spp.) by host interaction. Plant Disease Reporter 36, 84–6.Google Scholar
Sasser, J. N. (1980). Root-knot nematodes: A global menace to crop production. Plant Disease Reporter 64, 36–41.CrossRefGoogle Scholar
Sasser, J. N., Eisenback, J. D., Canter, C. C. & Triantaphyllou, A. A. (1983). The international Meloidogyne project – its goals and accomplishments. Annual Review of Phytopathology 21, 271–88.CrossRefGoogle Scholar
Sim, B. K. L., Piessens, W. F. & Wirth, D. F. (1986). A DNA probe cloned in Escherichia coli for the identification of Brugia malayi. Molecular and Biochemical Parasitology 19, 117–23.Google Scholar
Simpson, A. J. G., Sher, A. & McCutchan, I. F. (1982). The genome of Schistosoma mansoni: isolation of DNA, its size and repetitive sequences. Molecular and Biochemical Parasitology 6, 125–37.CrossRefGoogle ScholarPubMed
Short, J. M., Fernandez, J. M., Serge, J. A. & Huse, W. D. (1988). λ ZAP: A bacteriophage expression vector with in vivo excision properties. Nucleic Acids Research 16, 7583–660.CrossRefGoogle ScholarPubMed