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Spore ornamentation of Haplosporidium hinei n. sp. (Haplosporidia) in pearl oysters Pinctada maxima (Jameson, 1901)

Published online by Cambridge University Press:  04 February 2008

D. BEARHAM ,
Z. SPIERS ,
S. RAIDAL ,
J. B. JONES ,
E. M. BURRESON  and
P. K. NICHOLLS
D. BEARHAM*
Affiliation:
Fish Health Unit, Murdoch University, School of Veterinary and Biomedical Sciences, Murdoch Drive, Perth, Western Australia 6150
Z. SPIERS
Affiliation:
Fish Health Unit, Murdoch University, School of Veterinary and Biomedical Sciences, Murdoch Drive, Perth, Western Australia 6150
S. RAIDAL
Affiliation:
Charles Sturt University, School of Agriculture and Veterinary Science, Boorooma St, Wagga Wagga, NSW Australia
J. B. JONES
Affiliation:
Fish Health Unit, Animal Health Labs, Department of Fisheries, 3 Baron-Hay Court, South Perth, Western Australia 6151
E. M. BURRESON
Affiliation:
Virginia Institute of Marine Science, College of William and Mary, Gloucester Point, Virginia 23062, USA
P. K. NICHOLLS
Affiliation:
Fish Health Unit, Murdoch University, School of Veterinary and Biomedical Sciences, Murdoch Drive, Perth, Western Australia 6150
*
*Corresponding author: Fish Health Unit, Murdoch University, School of Veterinary and Biomedical Sciences, Murdoch Drive, Perth, Western Australia 6150. Tel: +61 08 9360 2479. E-mail: dbearham@hotmail.com
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Summary

An infection of pearl oysters, Pinctada maxima, attributed to a Haplosporidium sp. by Hine and Thorne (1998) has been detected on 3 occasions and is considered to represent a serious concern to the pearling industry in Australia. The spore ornamentation of the parasite was determined by scanning electron microscopy and transmission electron microscopy. Spores of the parasite were pleomorphic, or elongated 3·5–4 μm×2·5–3·0 μm in size. Two filaments were wound around the spore and originated from 2 ‘knob-like’ posterior thickenings. Both filaments passed up one side of the spore together until just below the operculum whereupon each split and passed obliquely under the lip of the opercula lid. Each filament wrapped around the spore 4 times. The posterior thickenings seem to appear late in the development of the spore and were composed of spore wall material. A second set of branching tubular filaments composed of a different material was observed on the spore body although not on mature spores possessing a ‘knob-like’ posterior thickening. The ornamentation on the spores of the pearl oyster parasite was unique amongst described haplosporidian species where spore ornamentation is known. The parasite is named in this manuscript as Haplosporidium hinei n. sp.

Keywords

Haplosporidiapearl oysterPinctada maximaHaplosporidium hinei n. sp.MinchiniaparasiteSEMaquaculture
Type
Original Articles
Information
Parasitology , Volume 135 , Issue 4 , April 2008 , pp. 521 - 527
Copyright
Copyright © Cambridge University Press 2008

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References

REFERENCES

ABARE (2007). Australian Fisheries Statistics 2006. Australian Bureau of Agricultural and Resource Economics, Canberra, Australia.Google Scholar
Azevedo, C. (1984). Ultrastructure of the spore of Haplosporidium lusitanicum sp. n. (Haplosporida, Haplosporidiidae), parasite of a marine mollusc. Journal of Parasitology 70, 358–371.CrossRefGoogle Scholar
Azevedo, C., Balseiro, P., Casal, G., Gestal, C., Aranguren, R., Stokes, N. A., Carnegie, R. B., Novoa, B., Burreson, E. M. and Figueras, A. J. (2006). Ultrastructural and molecular characterization of Haplosporidium montforti n. sp., parasite of the European abalone Haliotis tuberculata. Journal of Invertebrate Pathology 92, 23–32.CrossRefGoogle Scholar
Azevedo, C., Conchas, R. F. and Montes, J. (2003). Description of Haplosporidium edule n. sp. (Phylum Haplosporidia), a parasite of Cerastoderma edule (Mollusca, Bivalvia) with complex spore ornamentation. European Journal of Protistology 39, 161–167.CrossRefGoogle Scholar
Azevedo, C., Montes, J. and Corral, L. (1999). A revised description of Haplosporidium armoricanum, parasite of Ostrea edulis L. from Galicia, northwestern Spain, with special reference to the spore-wall filaments. Parasitological Research 85, 977–983.CrossRefGoogle Scholar
Bearham, D., Spiers, Z., Raidal, S., Jones, J. B. and Nicholls, P. K. (2007). Molecular characterisation of a Haplosporidian parasite infecting rock oysters Saccostrea cuccullata in north Western Australia. Journal of Invertebrate Pathology 95, 33–40.CrossRefGoogle ScholarPubMed
Burreson, E. M. (2001). Spore ornamentation of Haplosporidium pickfordi Barrow, 1961 (Haplosporidia), a parasite of freshwater snails in Michigan, USA. Journal of Eukaryote Microbiology 48, 622–626.CrossRefGoogle ScholarPubMed
Burreson, E. M. and Reece, K. S. (2006). Spore ornamentation of Haplosporidium nelsoni and Haplosporidium costale (Haplosporidia), and incongruence of molecular phylogeny and spore ornamentation in the Haplosporidia. Journal of Parasitology 92, 1295–1301.CrossRefGoogle ScholarPubMed
Carnegie, R. B. (2005). Effects in mollusc culture. In Marine Parasitology (ed. Rohde, K.), pp. 391–398. CSIRO Publishing, Collingwood, Victoria, Australia.Google Scholar
Carnegie, R. B., Burreson, E. M., Hine, P. M., Stokes, N. A., Audemard, C., Bishop, M. J. and Peterson, C. H. (2006). Bonamia perspora n. sp (Haplosporidia), a parasite of the oyster Ostreola equestris is the first Bonamia species known to produce spores. Journal of Eukaryotic Microbiology 53, 232–245.CrossRefGoogle Scholar
Hine, P. M. and Thorne, T. (1998). Haplosporidium sp. (Haplosporidia) in hatchery-reared pearl oysters, Pinctada maxima (Jameson, 1901), in north Western Australia. Journal of Invertebrate Pathology 71, 48–52.CrossRefGoogle Scholar
Hine, P. M. and Thorne, T. (2002). Haplosporidium sp. (Alveolata: Haplosporidia) associated with mortalities among rock oysters Saccostrea cuccullata in north Western Australia. Diseases of Aquatic Organisms 51, 123–133.CrossRefGoogle ScholarPubMed
Humphrey, J. D. and Norton, J. H. (2005). The Pearl Oyster Pinctada maxima. An atlas of the Functional Anatomy, Pathology and Histopathology. Northern Territory Department of Industry, Fisheries and Mines. Queensland Department of Primary Industries and Fisheries. Fisheries Research and Development Corporation, Darwin, Northern Territory, Australia.Google Scholar
Jones, J. B. and Creeper, J. H. (2006). Diseases of pearl oysters and other molluscs: A Western Australian perspective. Journal of Shellfish Research 25, 233–238.CrossRefGoogle Scholar
La Haye, C. A., Holland, N. D. and Mclean, N. (1984). Electron microscopic study of Haplosporidium comatulae n. sp. (Phylum Ascetospora: class Stellatosporea), A haplosporidian endoparasite of an Australian crinoid Oligometra serripinna (Phylum Echinodermata). Protistologica 20, 507–515.Google Scholar
Ormieres, R. (1980). Haplosporidium parisi n. sp Haplosporidie parasite de Serpula vermicularis L. etude ultrastructure de la spore. Protistologica 16, 467–474.Google Scholar
Perkins, F. O. (2000). Phylum Haplosporidia Caullery & Mesnil 1899. In An Illustrated Guide to the Protozoa, Vol. 2 (ed. Lee, J. J., Leedale, G. F. and Bradbury, P.), pp. 1328–1341. Society of Protozoologists, Lawrence, Kansas, USA.Google Scholar