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The structure and histochemistry of the cyst wall of the metacercaria of Fasciola hepatica L.

Published online by Cambridge University Press:  06 April 2009

K. E. Dixon
K. E. Dixon
Affiliation:
Department of Zoology, School of General Studies, The Australian National University, Canberra, A.C.T.
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Extract

The cyst wall which encloses the metacercaria of Fasciola hepatica consists of four major layers, one of which is further divisible into three sublayers. These layers are numbered I to IV, the first being the external layer. Layers I and II form the outer cyst which may be separated from the inner cyst formed from layers III and IV.

Layer I, the thick incomplete external layer, covers the metacercaria dorsally and laterally. It is composed of tanned protein.

Layer II is a thin fibrous layer closely adherent to the inner surface of layer I and is composed of mucoprotein and acid mucopolysaccharide.

Layer III is made up of three separate sublayers, the relative widths of which vary in different regions of the cyst. In general IIIa consists of mucoprotein, IIIb of acid mucopolysaccharide and IIIc of neutral mucopolysaccharide.

In the dorsal and lateral regions, layer IV appears to be formed of lamellae held in a protein–lipid matrix. The lamellae are composed of protein stabilized by disulphide linkages.

In the ventral region of layer IV there is a thickened mucopolysaccharide area, the ventral plug, through which the metacercaria excysts.

The relative importance of these resistant layers in providing protection against desiccation, toxic substances and attack by other organisms is discussed.

This work was carried out during the tenure of a Commonwealth Post Graduate Scholarship. The author is grateful to Professor J. D. Smyth and Dr J. A. Clegg for advice and encouragement during the course of this work and in preparation of the manuscript.

Type
Research Article
Information
Parasitology , Volume 55 , Issue 2 , May 1965 , pp. 215 - 226
Copyright
Copyright © Cambridge University Press 1965

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References

Alicata, J. E. (1938). Observations on the life history of Fasciola gigantica, the common liver fluke of cattle in Hawaii, and the intermediate host, Fossaria ollula. Bull. Hawaii agric. Exp. Stn, no. 80.CrossRefGoogle Scholar
Baker, J. R. (1960). Cytological Technique, 4th ed., 150 pp. London: Methuen and Co. Ltd.Google Scholar
Baldwin, E. (1937). In Perspectives in Biochemistry, 99 pp. (Ed. J., Needham and Green, D. E..) Cambridge University Press.Google Scholar
Barlow, C. H. (1925). The life cycle of the human intestinal fluke Fasciolopsis buski (Lankester). Am. J. Hyg. Monogr. (Ser. 4), 98 pp.Google Scholar
Barrnett, R. J. & Seligman, A. M. (1954). Histochemical demonstration of sulphydryl and disulphide groups of protein. J. natn. Cancer Inst. 14, 769–803.Google ScholarPubMed
Beaver, P. C. (1943). Studies on Protechinostoma mucronisertulatum n.g. n.n. (Psilostomum reflexae Feldman, 1941), a trematode (Echinostomidae) from the sora rail. J. Parasit. 29, 65–70.CrossRefGoogle Scholar
Bennett, H. S. (1951). The demonstration of thiol groups in certain tissues by means of a new coloured sulphydryl reagent. Anat. Rec. 110, 231–47.CrossRefGoogle Scholar
Bogitsh, B. J. (1962). The chemical nature of metacercarial cysts. I. Histological and histochemical observations on the cyst of Posthodiplostomum minimum. J. Parasit. 48, 55–60.CrossRefGoogle ScholarPubMed
Bonhag, P. F. (1955). Histochemical studies of the ovarian nurse tissues and oocytes of the milkweed bug, Oncopeltus fasciatus (Dallas). I. Cytology, nucleic acids, and carbohydrates. J. Morph. 96, 381–440.CrossRefGoogle Scholar
Brown, C. H. (1950). A review of the methods available for the determination of the types of forces stabilizing structural proteins in animals. Quart. J. micr. Sci. 91, 331–9.Google ScholarPubMed
Campbell, W. C. (1960). Presence of phenolase in the fasciolid metacercarial cyst. J. Parasit. 46, 848.CrossRefGoogle Scholar
Carleton, H. M. & Drury, R. A. B. (1957). Histological Technique, 343 pp., 3rd ed.London: Oxford University Press.Google Scholar
Chiffelle, T. L. & Putt, F. A. (1951). Propylene and ethylene glycol as solvents for Sudan IV and Sudan Black B. Stain Tech. 26, 51–6.CrossRefGoogle ScholarPubMed
Dawes, B. (1961). On the early stages of Fasciola hepatica penetrating into the liver of an experimental host, the mouse. A histological picture. J. Helminth. (R. T. Leiper Supplement), pp. 41–52.CrossRefGoogle Scholar
Dawes, B. (1963). The migration of juvenile forms of Fasciola hepatica L. through the wall of the intestines in the mouse, with some observations on food and feeding. Parasitology, 53, 109–22.CrossRefGoogle Scholar
Dinnik, J. A. & Dinnik, N. N. (1961). On the morphology and life history of Fasciola nyanzae Leiper, 1910 from the hippopotamus. J. Helminth. (R. T. Leiper Supplement), pp. 53–62.CrossRefGoogle ScholarPubMed
Dixon, K. E. (1964). Excystment of metacercariae of Fasciola hepatica L. in vitro. Nature, Lond., 202, 1240–1.CrossRefGoogle Scholar
Dixon, K. E. & Mercer, E. H. (1964). The fine structure of the cyst wall of the metacercaria of Fasciola hepatica L. Quart. J. micr. Sci. (in the Press).Google Scholar
Durie, P. H. (1953). The paramphistomes (Trematoda) of Australian ruminants. II. The life history of Ceylonocotyle streptocoelium (Fischoeder) Näsmark and of Paramphistomum ichikawai Fukui. Aust. J. Zool. 1, 193–222.CrossRefGoogle Scholar
Feldman, S. I. (1941). Studies on the morphology and biology of a psilostome fluke. J. Parasit. 27, 525–33.CrossRefGoogle Scholar
Gurr, E. (1958). Methods of Analytical Histology and Histochemistry, 327 pp. London: Leonard Hill (Books) Ltd.Google Scholar
Herber, E. C. (1950). Studies on the biochemistry of cyst envelopes of the fluke, Notocotylus urbanensis. Proc. Pa Acad. Sci. 24, 140–2.Google Scholar
Hunter, G. W. III & Hunter, W. S. (1940). Studies on the development of the metacercaria and the nature of the cyst of Posthodiplostomum minimum (MacCallum, 1921) (Trematoda: Strigeata). Trans. Amer. micr. Soc. 59, 52–63.CrossRefGoogle Scholar
Leblond, C. P., Glegg, R. E. & Eidinger, D. (1957). Presence of carbohydrates with free 1,2-glycol groups in sites stained by the periodic acid-Schiff technique. J. Histochem. Cytochem. 5, 445–58.CrossRefGoogle ScholarPubMed
Lee, C. L. & Lewert, R. M. (1957). Studies on the presence of mucopolysaccharidase in penetrating helminth larvae. J. infect. Dis. 101, 287–94.CrossRefGoogle ScholarPubMed
Lengy, G. (1960). Study on Paramphistomum microbothrium Fischoeder, 1901, a rumen parasite of cattle in Israel. Bull. Res. Coun. Israel (Sect. B), 9, 71–130.Google Scholar
Lenhoff, H. M., Schroeder, R. & Leigh, W. H. (1960). The collagen-like nature of metacercarial cysts of a new species of Ascocotyle. J. Parasit. 46 (Sect. 2), 36. (Abstract.)Google Scholar
Lillie, R. D. (1944). Various oil soluble dyes as fat stains in the supersaturated isopropanol technic. Stain Tech. 19, 55–8.CrossRefGoogle Scholar
Lynch, D. L. & Bogitsh, B. J. (1962). The chemical nature of metacercarial cysts. II. Biochemical investigations on the cyst of Posthodiplostomum minimum. J. Parasit. 48, 241–3.CrossRefGoogle ScholarPubMed
Martin, W. E. (1956). The life cycle of Catatropis johnstoni n.sp. (Trematoda: Notocotylidae). Trans. Amer. micr. Soc. 75, 117–28.CrossRefGoogle Scholar
McManus, J. F. A. (1946). Histological demonstration of mucin after periodic acid. Nature, Lond., 158, 202.CrossRefGoogle ScholarPubMed
Mowry, R. W. (1963). The special value of methods that colour both acidic and vicinal hydroxyl groups in the histochemical study of mucins. With revised directions for the colloidal iron stain, the use of Alcian blue G8X and their combinations with the periodic acid-Schiff reaction. Ann. N.Y. Acad. Sci. 106, 402–23.CrossRefGoogle Scholar
Pearse, A. G. E. (1961). Histochemistry: Theoretical and Applied, 998 pp., 2nd ed.London: J. and A. Churchill Ltd.Google Scholar
Rees, F. G. (1932). An investigation into the occurrence, structure and life-histories of the trematode parasites of four species of Lymnaea (L. truncatula (Müll.), L. pereger (Müll.), L. palustris (Müll.) and L. stagnalis (Linné)), and Hydrobia jenkinsi (Smith) in Glamorgan and Monmouth. Proc. zool. Soc. Lond. 1–32.Google Scholar
Rees, G. (1937). The anatomy and encystment of Cercaria purpurae Lebour, 1911. Proc. zool. Soc. Lond. 107, 65–73.CrossRefGoogle Scholar
Rogers, G. E. (1959). Electron microscope studies of hair and wool. Ann. N.Y. Acad. Sci. 83, 378–99.CrossRefGoogle ScholarPubMed
Rothschild, M. (1936). The process of encystment of a cercaria parasitic in Lymnaea tenera euphratica. Parasitology, 28, 56–62.CrossRefGoogle Scholar
Schumacher, W. (1938). Untersuchungen über den Wanderungsweg und die Entwicklung von Fasciola hepatica L. in Endwirt. Z. Parasitenk. 10, 608–43.CrossRefGoogle Scholar
Shirai, M. (1927). The biological observation on the cysts of Fasciola hepatica and the route of migration of young worms in the final host. Sci. Rep. Inst. inf. Dis. Tokyo Univ. 6, 511–24.Google Scholar
Singh, K. S. & Lewert, R. M. (1959). Observations on the formation and chemical nature of metacercarial cysts of Notocotylus urbanensis. J. infect. Dis. 104, 138–41.CrossRefGoogle Scholar
Sinitsin, D. F. (1933). Studien über die Phylogenie der Trematoden. VI. The life histories of some American liver flukes. Z. Parasitenk. 6, 170–91.CrossRefGoogle Scholar
Smyth, J. D. (1954). A technique for the histochemical demonstration of polyphenol oxidase and its application to egg-shell formation in helminths and byssus formation in Mytilus. Quart. J. micr. Sci. 95, 139–52.Google Scholar
Spicer, S. S. (1963). Histochemical differentiation of mammalian mucopolysaccharides. Ann. N.Y. Acad. Sci. 106, 379–88.CrossRefGoogle Scholar
Swales, W. E. (1935). The life cycle of Fascioloides magna (Bassi, 1875), the large liver fluke of ruminants of Canada, with observations on the bionomics of the larval stages and the intermediate hosts, pathology of fascioloidiasis magna, and control measures. Can. J. Res. 12, 177–215.CrossRefGoogle Scholar
Thapar, G. S. & Tandon, R. S. (1952). On the life history of liver-fluke, Fasciola gigantica Cobbold, 1855 in India. Indian J. Helminth. 4, 77–112.Google Scholar
Urquhart, G. M. (1954). The rabbit as host in experimental fascioliasis. Expl Parasit. 3, 38–44.CrossRefGoogle ScholarPubMed
Wallace, F. G. (1939). The metacercaria of Amphimerus elongatus Gower (Trematoda: Opisthorchiidae). J. Parasit. 25, 491–4.CrossRefGoogle Scholar
Weesner, F. M. (1960). General Zoological Microtechnique, 230 PP. Baltimore: The Williams and Wilkins Coy.Google Scholar
Wesenberg-Lund, C. (1934). Contributions to the development of the Trematoda Digenea. Part II. The biology of the freshwater cercariae in Danish fresh waters. K. danske Vidensk Selsk. Skr. 9,5, 223 pp.Google Scholar
Williams, G. & Jackson, D. S. (1956). Two organic fixatives for acid mucopolysaccharides. Stain Tech. 31, 189–91.CrossRefGoogle Scholar
Wright, W. R. (1927). Studies on larval trematodes from North Wales. Part I. Observations on the rediae, cercaria and cyst of Fasciola hepatica. Ann. trop. Med. Parasit. 21, 47–56.CrossRefGoogle Scholar