Hostname: page-component-78c5997874-xbtfd Total loading time: 0 Render date: 2024-11-10T16:38:32.325Z Has data issue: false hasContentIssue false
  • English
  • Français

A study of the effect of surface damage on the uptake of Texas Red–BSA by schistosomula of Schistosoma mansoni

Published online by Cambridge University Press:  10 March 2003

H. H. C. TAN ,
J. A. THORNHILL ,
B. H. AL-ADHAMI ,
A. AKHKHA  and
J. R. KUSEL
H. H. C. TAN
Affiliation:
Division of Biochemistry and Molecular Biology, The Davidson Building, Institute of Biomedical and Life Science, University of Glasgow, Glasgow G12 8QQ, UK
J. A. THORNHILL
Affiliation:
Division of Biochemistry and Molecular Biology, The Davidson Building, Institute of Biomedical and Life Science, University of Glasgow, Glasgow G12 8QQ, UK
B. H. AL-ADHAMI
Affiliation:
Division of Biochemistry and Molecular Biology, The Davidson Building, Institute of Biomedical and Life Science, University of Glasgow, Glasgow G12 8QQ, UK
A. AKHKHA
Affiliation:
Division of Biochemistry and Molecular Biology, The Davidson Building, Institute of Biomedical and Life Science, University of Glasgow, Glasgow G12 8QQ, UK
J. R. KUSEL
Affiliation:
Division of Biochemistry and Molecular Biology, The Davidson Building, Institute of Biomedical and Life Science, University of Glasgow, Glasgow G12 8QQ, UK
Get access Rights & Permissions [Opens in a new window]

Abstract

In this paper we describe the effect of poly-L-lysines of different molecular weight on the schistosomula. In the control sample, the schistosomula of Schistosoma mansoni take up fluorescent Texas Red conjugated to bovine serum albumin (TxR–BSA) into the gut. Following slight damage by 24·0 kDa poly-L-lysine, a high proportion of schistosomula take up fluorescent TxR–BSA into the excretory system. Subsequently, the dye diffused into the bodies of the schistosomula. We suspected that this diffusion involved the process of endocytosis so we investigated this with the use of endocytosis inhibitor, Latrunculin A. Addition of the endocytosis inhibitor Latrunculin A following poly-L-lysine treatment inhibited gut uptake of TxR–BSA as well as the diffusion of excretory-ingested TxR–BSA molecules.

Keywords

Schistosoma mansonischistosomulaTexas Red–BSAexcretory systemsurface membraneLatrunculin A
Type
Research Article
Information
Parasitology , Volume 126 , Issue 3 , March 2003 , pp. 235 - 240
Copyright
2003 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)