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Study on the mitochondrial apoptosis pathways of small cell lung cancer H446 cells induced by Trichinella spiralis muscle larvae ESPs

Published online by Cambridge University Press:  11 January 2017

JINGMEI LUO ,
LI YU ,
GUANGCHENG XIE ,
DAN LI ,
MENG SU ,
XUERONG ZHAO  and
LUANYING DU
JINGMEI LUO
Affiliation:
Department of Pathogenic Biology, Chengde Medical University, Chengde 06700, Hebei, China
LI YU
Affiliation:
Department of Pathogenic Biology, Chengde Medical University, Chengde 06700, Hebei, China
GUANGCHENG XIE
Affiliation:
Department of Pathogenic Biology, Chengde Medical University, Chengde 06700, Hebei, China
DAN LI
Affiliation:
Department of Pathogenic Biology, Chengde Medical University, Chengde 06700, Hebei, China
MENG SU
Affiliation:
Department of Pathogenic Biology, Chengde Medical University, Chengde 06700, Hebei, China
XUERONG ZHAO
Affiliation:
Department of Immunology, Chengde Medical University, Chengde 06700, Hebei, China
LUANYING DU*
Affiliation:
Department of Pathogenic Biology, Chengde Medical University, Chengde 06700, Hebei, China
*
*Corresponding author: Luan-ying Du, Department of Pathogenic Biology, Chengde Medical University, Chengde 06700, Hebei, China. E-mail: luanyingd@126.com
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Summary

Trichinella spiralis (T.spiralis) muscle-larva (ML) excretory–secretory proteins (ESPs) contain antitumour-active substances. ESPs have been shown to inhibit tumour growth. To explore the effects of these proteins on small cell lung cancer cells and the possible mechanisms of their antineoplastic action, H446 SCLC cells were co-cultured with different concentrations of T. spiralis ML ESPs for 12, 24 and 48 h. Our results showed that T. spiralis ML ESPs significantly inhibited H446 cell proliferation, which was dose-and time-dependent. The results of flow cytometry testing indicate a clear apoptosis trend in H446 cells co-cultured with ESPs for 24 h. Reverse transcription polymerase chain reaction and Western blotting results showed increased expression of pro-apoptosis genes Bax, Cyt-C, Apaf-1, caspase-9 and caspase-3, compared with the negative control group, and decreased the expression of anti-apoptosis genes Bcl-2 and Livin. Our results suggest that T. spiralis ML ESPs can induce apoptosis in H446 cells through a mitochondrial pathway, which may be a mechanism of antineoplastic action in T. spiralis ML ESPs.

Keywords

Apoptosisexcretory–secretory proteinsmitochondrial pathwaysmall cell lung cancerTrichinella spiralis
Type
Research Article
Information
Parasitology , Volume 144 , Issue 6 , May 2017 , pp. 793 - 800
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Copyright
Copyright © Cambridge University Press 2017 

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