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The synthesis of a variant-specific antigen by Trypanosoma brucei in vitro

Published online by Cambridge University Press:  06 April 2009

D. W. Taylor  and
G. A. M. Cross
D. W. Taylor
Affiliation:
Medical Research Council Biochemical Parasitology Unit, Molteno Institute, Dowing Street, Cambridge CB2 3EE
G. A. M. Cross
Affiliation:
Medical Research Council Biochemical Parasitology Unit, Molteno Institute, Dowing Street, Cambridge CB2 3EE
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Extract

A variant-specific surface antigen from a cloned population of Trypanosoma brucei S 42 has been isolated and partically characterized. [35S]L-methionine was found to be incorporated into this material by cells incubated in vitro in a chemically defined medium. Incorporation of [35S]L-methionine was inhibited by cycloheximide and puromycin at concentrations which are known to specifically inhibit protein synthesis in other systems. The rate of synthesis of the variant-specific antigen in vitro has been estimated to be about 8% of the rate in vivo. Newly synthesized [35S]L-methionine-labelled variant-specific antigen was incorporated into the surface coat.

Type
Research Article
Information
Parasitology , Volume 74 , Issue 1 , February 1977 , pp. 47 - 60
Copyright
Copyright © Cambridge University Press 1977

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