Hostname: page-component-cd9895bd7-mkpzs Total loading time: 0 Render date: 2024-12-28T13:52:40.108Z Has data issue: false hasContentIssue false
  • English
  • Français

The TcTASV proteins are novel promising antigens to detect active Trypanosoma cruzi infection in dogs

Published online by Cambridge University Press:  13 May 2016

N. FLORIDIA-YAPUR ,
M. MONJE RUMI ,
P. RAGONE ,
J. J. LAUTHIER ,
N. TOMASINI ,
A. ALBERTI D'AMATO ,
P. DIOSQUE ,
R. CIMINO ,
J. D. MARCO  and
P. BARROSO
...Show all authors
N. FLORIDIA-YAPUR
Affiliation:
Cátedra de Química Biológica, Facultad de Ciencias Naturales, Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina Instituto de Investigaciones de Enfermedades Tropicales (IIET), Sede Regional Orán, Universidad Nacional de Salta, Alvarado 751, Orán-Salta (4530), Argentina
M. MONJE RUMI
Affiliation:
Instituto de Patología Experimental (IPE-CONICET), Facultad de Ciencias de la Salud, Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina
P. RAGONE
Affiliation:
Instituto de Patología Experimental (IPE-CONICET), Facultad de Ciencias de la Salud, Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina
J. J. LAUTHIER
Affiliation:
Instituto de Patología Experimental (IPE-CONICET), Facultad de Ciencias de la Salud, Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina
N. TOMASINI
Affiliation:
Instituto de Patología Experimental (IPE-CONICET), Facultad de Ciencias de la Salud, Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina
A. ALBERTI D'AMATO
Affiliation:
Instituto de Patología Experimental (IPE-CONICET), Facultad de Ciencias de la Salud, Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina
P. DIOSQUE
Affiliation:
Instituto de Patología Experimental (IPE-CONICET), Facultad de Ciencias de la Salud, Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina
R. CIMINO
Affiliation:
Cátedra de Química Biológica, Facultad de Ciencias Naturales, Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina Instituto de Investigaciones de Enfermedades Tropicales (IIET), Sede Regional Orán, Universidad Nacional de Salta, Alvarado 751, Orán-Salta (4530), Argentina
J. D. MARCO
Affiliation:
Instituto de Patología Experimental (IPE-CONICET), Facultad de Ciencias de la Salud, Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina
P. BARROSO
Affiliation:
Instituto de Patología Experimental (IPE-CONICET), Facultad de Ciencias de la Salud, Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina
D. O. SANCHEZ
Affiliation:
Instituto de Investigaciones Biotecnológicas “Dr. Rodolfo A. Ugalde” (IIB-INTECH), Universidad Nacional de San Martín, UNSAM-CONICET, Av. 25 de Mayo, Partido de San Martín, Buenos Aires (1650), Argentina
J. R. NASSER
Affiliation:
Cátedra de Química Biológica, Facultad de Ciencias Naturales, Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina Instituto de Investigaciones de Enfermedades Tropicales (IIET), Sede Regional Orán, Universidad Nacional de Salta, Alvarado 751, Orán-Salta (4530), Argentina
V. TEKIEL*
Affiliation:
Instituto de Investigaciones Biotecnológicas “Dr. Rodolfo A. Ugalde” (IIB-INTECH), Universidad Nacional de San Martín, UNSAM-CONICET, Av. 25 de Mayo, Partido de San Martín, Buenos Aires (1650), Argentina
*
*Corresponding author: Instituto de Investigaciones Biotecnológicas “Dr. Rodolfo A. Ugalde” (IIB-INTECH), Universidad Nacional de San Martín, UNSAM-CONICET, Av. 25 de Mayo y Francia, Partido de San Martín, Buenos Aires (1650), Argentina. E-mail: valet@iib.unsam.edu.ar or vtekiel@gmail.com
Get access Rights & Permissions [Opens in a new window]

Summary

In regions where Chagas disease is endemic, canine Trypanosoma cruzi infection is highly correlated with the risk of transmission of the parasite to humans. Herein we evaluated the novel TcTASV protein family (subfamilies A, B, C), differentially expressed in bloodstream trypomastigotes, for the detection of naturally infected dogs. A gene of each TcTASV subfamily was cloned and expressed. Indirect enzyme-linked immunosorbent assays (ELISA) were developed using recombinant antigens individually or mixed together. Our results showed that dogs with active T. cruzi infection differentially reacted against the TcTASV-C subfamily. The use of both TcTASV-C plus TcTASV-A proteins (Mix A+C-ELISA) enhanced the reactivity of sera from dogs with active infection, detecting 94% of the evaluated samples. These findings agree with our previous observations, where the infected animals exhibited a quick anti-TcTASV-C antibody response, coincident with the beginning of parasitaemia, in a murine model of the disease. Results obtained in the present work prove that the Mix A+C-ELISA is a specific, simple and cheap technique to be applied in endemic areas in screening studies. The Mix A+C-ELISA could help to differentially detect canine hosts with active infection and therefore with high impact in the risk of transmission to humans.

Keywords

Trypanosoma cruzidogsTcTASV antigensimmunodiagnosis
Type
Research Article
Information
Parasitology , Volume 143 , Issue 11 , September 2016 , pp. 1382 - 1389
Check for updates
Copyright
Copyright © Cambridge University Press 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Araújo, F. M. G., Bahia, M. T., Magalhaes, N. M., Martins-Filho, O. A., Veloso, V. M., Carneiro, C. M., Tafuri, W. L. and Lana, M. (2002). Follow-up of experimental chronic Chagas’ disease in dogs: use of polymerase chain reaction (PCR) compared with parasitological and serological methods. Acta Tropica 81, 21–31.Google Scholar
Barroso, P. A., Nevot, M. C., Hoyos, C. L., Locatelli, F. M., Lauthier, J. J., Ruybal, P., Cardozo, R. M., Russo, P. D., Vassiliades, C. N., Mora, M. C., Estévez, J. O., Hashiguchi, Y., Korenaga, M., Basombrío, M. A. and Marco, J. D. (2015a). Genetic and clinical characterization of canine leishmaniasis caused by Leishmania (Leishmania) infantum in northeastern Argentina. Acta Tropica 150, 218–223.Google Scholar
Barroso, P. A., Marco, J. D., Locatelli, F. M., Cardozo, R. M., Hoyos, C. L., Mora, M. C., García Bustos, M. F., López-Quiroga, I., Mimori, T., Gentile, A. G., Barrio, A. B., Korenaga, M., Hashiguchi, Y. and Basombrío, M. A. (2015b). Visceral Leishmaniasis caused by Leishmania infantum in Salta, Argentina: possible reservoirs and vectors. American Journal of Tropical Medicine and Hygiene 93, 334–339.Google Scholar
Bernabó, G., Levy, G., Ziliani, M., Caeiro, L. D., Sánchez, D. O. and Tekiel, V. (2013). TcTASV-C, a protein family in Trypanosoma cruzi that is predominantly trypomastigote-stage specific and secreted to the medium. Plos One 8, 1–10.Google Scholar
Caballero, Z. C.; Sousa, O. E.; Marques, W. P.; Saez-Alquezar, A.; Umezawa, E. S. (2007). Evaluation of serological tests to identify Trypanosoma cruzi infection in humans and determine cross-reactivity with Trypanosoma rangeli and Leishmania spp. Clinical and Vaccine Immunology. 14 (8), 1045–1049.Google Scholar
Cardinal, M. V., Castañera, M., Lauricella, M., Cecere, M., Ceballos, L., Vazquez-Prokopec, G., Kitron, U. and Gürtler, R. (2006a). A prospective study of the effects of sustained vector surveillance following community-wide insecticide application on Trypanosoma cruzi infection of dogs and cats in rural northwestern Argentina. The American Society of Tropical Medicine and Hygiene 75, 753–761.Google Scholar
Cardinal, M. V., Reithinger, R. and Gürtler, R. E. (2006b). Use of an immunochromatographic dipstick test for rapid detection of Trypanosoma cruzi in sera from animal reservoir hosts. Journal of Clinical Microbiology 44, 3005–3007.Google Scholar
Castañera, M. B., Lauricella, M. A., Chuit, R. and Gürtler, R. E. (1998). Evaluation of dogs as sentinels of the transmission of Trypanosoma cruzi in a rural area of north-western Argentina. Annals of Tropical Medicine and Parasitology 92, 671–683.Google Scholar
Cimino, R. O., Monje Rumi, M., Ragone, P., Lauthier, J. J., Alberti D́ Amato, A., Lopez Quiroga, I. R., Gil, J. F., Cajal, S. P., Acosta, N., Juarez, M., Krolewiecki, A., Orellana, V., Zacca, R., Marcipar, I., Diosque, P. and Nasser, J. R. (2011). Immuno-enzimatic evaluation of the recombinant TSSA-II protein of Trypanosoma cruzi in dogs and human sera: a tool for epidemiological studies. Parasitology 138, 1–8.Google Scholar
Cimino, R. O., Diosque, P., Lopez Quiroga, I. R., Gil, J. F. and Nasser, J. R. (2012). Evaluación inmunoenzimática del antígeno recombinante SAPA en perros infectados naturalmente por Trypanosoma cruzi . Revista Argentina de Microbiologia 44, 1–5.Google Scholar
Crowther, J. R. (2009). The ELISA Guidebook. Second. Humana Press, Hatfield, Hertfordshire, UK.Google Scholar
Da Silveira, J. F., Umezawa, E. S. and Luquetti, A. O. (2001). Chagas disease: recombinant Trypanosoma cruzi antigens for serological diagnosis. Trends in Parasitology 17, 286–291.Google Scholar
Enriquez, G. F., Cardinal, M. V., Orozco, M. M., Schijman, A. G. and Gürtler, R. E. (2013). Detection of Trypanosoma cruzi infection in naturally infected dogs and cats using serological, parasitological and molecular methods. Acta Tropica 126, 211–217.Google Scholar
Enriquez, G. F., Bua, J., Orozco, M. M., Wirth, S., Schijman, A. G., Gürtler, R. E. and Cardinal, M. V. (2014). High levels of Trypanosoma cruzi DNA determined by qPCR and infectiousness to Triatoma infestans support dogs and cats are major sources of parasites for domestic transmission. Infection, Genetics and Evolution 25, 36–43.CrossRefGoogle ScholarPubMed
Floridia-Yapur, N., Vega-Benedetti, F., Monje Rumi, M., Ragone, P., Lauthier, J., Tomasini, N., Alberti D́ Amato, A., Lopez Quiroga, I., Diosque, P., Marcipar, I., Nasser, J. and Cimino, R. (2014). Evaluation of recombinant antigens of Trypanosoma cruzi to diagnose infection in naturally infected dogs from Chaco region, Argentina. Parasite Immunology 36, 694–699.Google Scholar
García, E. A., Ziliani, M., Agüero, F., Bernabó, G., Sánchez, D. O. and Tekiel, V. (2010). TcTASV: a novel protein family in Trypanosoma cruzi identified from a subtractive trypomastigote cDNA library. PLoS Neglected Tropical Diseases 4, 6.Google Scholar
Gürtler, R. E. (2009). Sustainability of vector control strategies in the Gran Chaco Region: current challenges and possible approaches. Memórias do Instituto Oswaldo Cruz 104, 52–59.Google Scholar
Gürtler, R. E., Cecere, M. C., Lauricella, M. A., Petersen, R., Chuit, R., Segura, E. L. and Cohen, J. E. (2005). Incidence of Trypanosoma cruzi infection among children following domestic reinfestation after insecticide spraying in rural northwestern Argentina. American Journal of Tropical Medicine and Hygiene 73, 95–103.Google Scholar
Gürtler, R. E., Cecere, M. C., Lauricella, M. A., Cardinal, M. V., Kitron, U. and Cohen, J. E. (2007). Domestic dogs and cats as sources of Trypanosoma cruzi infection in rural northwestern Argentina. Parasitology 134, 69–82.Google Scholar
Gürtler, R. E., Ceballos, L. A., Ordoñez-Krasnowski, P., Lanati, L. A., Stariolo, R. and Kitron, U. (2009). Strong host-feeding preferences of the vector Triatoma infestans modified by vector density: implications for the epidemiology of Chagas disease. PLoS Neglected Tropical Diseases 3, 1–12.Google Scholar
Lauricella, M. A., Wisnivesky-Colli, C., Gürtler, R. E., Petersen, R., Bujas, M. and Segura, E. L. (1993). Standarization of serological tests for detecting anti-Trypanosoma cruzi antibodies in dogs. Memórias do Instituto Oswaldo Cruz 88, 413–417.Google Scholar
Lauricella, M. A., Castañera, M. B., Gürtler, R. E. and Segura, E. L. (1998). Immunodiagnosis of Trypanosoma cruzi (Chagas’ disease) infection in naturally infected dogs. Memórias do Instituto Oswaldo Cruz 93, 501–507.Google Scholar
Linnet, K.; Bossuyt, P. M. M.; Moons, K. G. M. and Reitsma, J. B. (2012). Quantifying the accuracy of a diagnostic test or marker. Clinical Chemistry 58, 1292–1301.Google Scholar
Menezes-Souza, D., de Oliveira Mendes, T. A., de Souza Gomes, M. dS., Bartholomeu, D. C., Fujiwara, R. T. (2015). Improving serodiagnosis of human and canine Leishmaniasis with recombinant Leishmania braziliensis Cathepsin L-like protein and a synthetic peptide containing its linear B-cell Epitope. PLoS Negl Trop Dis 9(1), e3426.Google Scholar
Monje-Rumi, M., Pérez Brandán, C., Ragone, P., Tomasini, N., Lauthier, J., Alberti D́ Amato, A., Cimino, R., Orellana, V., Basombrío, M. and Diosque, P. (2015). Trypanosoma cruzi diversity in the Gran Chaco: mixed infections and differential host distribution of TcV and TcVI. Infection, Genetics and Evolution 29, 53–59.Google Scholar
Moretti, E., Castro, I., Franceschi, C. and Basso, B. (2010). Chagas disease: serological and electrocardiographic studies in Wichi and Creole communities of Misión Nueva Pompeya, Chaco, Argentina. Memórias do Instituto Oswaldo Cruz 105, 621–626.Google Scholar
Morillo, C., Marin-Neto, J., Avezum, A., Sosa-Estani, S., Rassi, A., Rosas, F., Villena, E., Quiroz, R., Bonilla, R., Britto, C., Guhl, F., Velazquez, E., Bonilla, L., Meeks, B., Rao-Melacini, P., Pogue, J., Mattos, A., Lazdins, J., Rassi, A., Connolly, S. J. and Yusuf, S. (2015). Randomized trial of benznidazole for chronic Chagas’ cardiomyopathy. The New England Journal of Medicine 373, 1295–1306.Google Scholar
Samuels, A., Clark, E., Galdos-Cardenas, G., Wiegand, R., Ferrufino, L., Menacho, S., Gil, J., Spicer, J., Budde, J., Levy, M., Bozo, R., Gilman, R. and Bern, C. (2013). Epidemiology of and impact of insecticide spraying on Chagas Disease in communities in the Bolivian Chaco. PLoS Neglected Tropical Diseases 7, 1–11.Google Scholar
Umezawa, E. S., Bastos, S. F., Camargo, M. E., Yamauchi, L. M., Santos, M. R., Gonzalez, A., Zingales, B., Levin, M. J., Sousa, O., Rangel-Aldao, R. and da Silveira, J. F. (1999). Evaluation of recombinant antigens for serodiagnosis of Chagas’ disease in South and Central America. Journal of Clinical Microbiology 37, 1554–60.Google Scholar
Umezawa, E. S., Souza, A. I., Pinedo-Cancino, V., Marcondes, M., Marcili, A., Camargo, L. M., Camacho, A. A., Stolf, A. M. and Teixeira, M. M. (2009). TESA-blot for the diagnosis of Chagas disease in dogs from co-endemic regions for Trypanosoma cruzi, Trypanosoma evansi and Leishmania chagasi . Acta Tropica 111, 15–20.Google Scholar
World Health Organization (2006). Estimación cuantitativa de la Enfermedad de Chagas en las Américas. Technical Report OPS/HDM/CD/425–06. OPS/HDM/CD, Montevideo, Uruguay.Google Scholar