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Temporal dynamics of spore release of the crayfish plague pathogen from its natural host, American spiny-cheek crayfish (Orconectes limosus), evaluated by transmission experiments

Published online by Cambridge University Press:  21 February 2013

J. SVOBODA ,
E. KOZUBÍKOVÁ-BALCAROVÁ ,
A. KOUBA ,
M. BUŘIČ ,
P. KOZÁK ,
J. DIÉGUEZ-URIBEONDO  and
A. PETRUSEK
J. SVOBODA
Affiliation:
Department of Ecology, Faculty of Science, Charles University in Prague, Viničná 7, Prague 2, CZ-12844, Czech Republic
E. KOZUBÍKOVÁ-BALCAROVÁ
Affiliation:
Department of Ecology, Faculty of Science, Charles University in Prague, Viničná 7, Prague 2, CZ-12844, Czech Republic
A. KOUBA
Affiliation:
South Bohemian Research Centre of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, Vodňany, CZ-38925, Czech Republic
M. BUŘIČ
Affiliation:
South Bohemian Research Centre of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, Vodňany, CZ-38925, Czech Republic
P. KOZÁK
Affiliation:
South Bohemian Research Centre of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, Vodňany, CZ-38925, Czech Republic
J. DIÉGUEZ-URIBEONDO
Affiliation:
Departamento de Micología, Real Jardín Botánico CSIC, Plaza Murillo 2, 28014 Madrid, Spain
A. PETRUSEK*
Affiliation:
Department of Ecology, Faculty of Science, Charles University in Prague, Viničná 7, Prague 2, CZ-12844, Czech Republic
*
*Corresponding author: Department of Ecology, Faculty of Science, Charles University in Prague, Viničná 7, Prague 2, CZ-12844, Czech Republic. Tel: +420 602 656 937. Fax: +420 221 951 673. E-mail: petrusek@cesnet.cz
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Summary

The crayfish plague pathogen, Aphanomyces astaci, is one of the most serious threats to indigenous European crayfish species. The North American invasive spiny-cheek crayfish, Orconectes limosus, is an important source of this pathogen in central and western Europe. We evaluated potential changes in A. astaci spore release rate from infected individuals of this species by experiments investigating the pathogen transmission to susceptible noble crayfish, Astacus astacus. We filtered defined volumes of water regularly to quantify spore concentration, and sampled crayfish tissues at the end of the experiment. The filters and tissues were then tested for the presence of A. astaci DNA by species-specific quantitative PCR. Additionally, we tested the efficiency of horizontal transmission to apparently uninfected O. limosus. The experiments confirmed that A. astaci can be transmitted to susceptible crayfish during intermoult periods, and that the pathogen was more frequently detected in noble crayfish recipients than in American ones. The pathogen spore concentrations substantially varied in time, and significantly increased during moulting of infected hosts. Our study strengthens the evidence that although the likelihood of crayfish plague transmission by water transfer from localities with infected American crayfish might increase when these are moulting or dying, no time-periods can be proclaimed safe.

Keywords

Aphanomyces astaciOrconectes limosusAstacus astacuscrayfish plague transmissionreal-time PCRhost moultingsporulation
Type
Research Article
Information
Parasitology , Volume 140 , Issue 6 , May 2013 , pp. 792 - 801
Copyright
Copyright © Cambridge University Press 2013

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